<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The present study has been performed to illustrate the role and mechanism of microRNA-147b (<italic>miR-147b</italic>) in the cellular viability and apoptosis of gastric cancer (GC) cells. The GC tissues of 50 patients with complete data and the adjacent tissues were selected from Shanxi Cancer Hospital, and 3 pairs of tissues were randomly selected for microarray detection of high-expressing microRNAs. The expressions of <italic>miR-147b</italic> were quantified in numerous GC cell lines, i.e., BGC-823, SGC-7901, AGS, MGC-803 and MKN-45, normal tissue cell lines and 50 pairs of gastric cancer tissues. Moreover, two cell lines of <italic>miR-147b</italic> high-expressing used PCR quantitative analysis were selected for transfection experiments. The differentially expressed <italic>miR-147b</italic> was screened from 3 pairs of samples by miRNA chip. The expression of<italic>miR-147b</italic> was found highly expressed in gastric cancer tissues of 50 pairs of gastric cancer and adjacent tissues. The <italic>miR-147b</italic> found in diverse range in each of GC cell line. Therefore, two cell lines, BGC-823 and MGC-803, with relatively high expression levels of <italic>miR-147b</italic> were selected for further analysis and research. Scratch analysis results showed that compared with <italic>miR-147b</italic> NC, the <italic>miR-147b</italic> inhibitor group inhibited GC cell growth and reduced cell migration. The early apoptosis of MGC-803, and BGC-823 cells was enhanced by <italic>miR-147b</italic> inhibitor. <italic>miR-147b</italic> inhibitor significantly repressed the proliferation of BGC-823 and MGC-803 cells. Our study showed that the high expression of <italic>miR-147b</italic> is positively correlated with the occurrence and development of gastric cancer.</p></abstract>

The present study has been performed to illustrate the role and mechanism of microRNA-147b (miR-147b) in the cellular viability and apoptosis of gastric cancer (GC) cells. The GC tissues of 50 patients with complete data and the adjacent tissues were selected from Shanxi Cancer Hospital, and 3 pairs of tissues were randomly selected for microarray detection of high-expressing microRNAs. The expressions of miR-147b were quantified in numerous GC cell lines, i.e., BGC-823, SGC-7901, AGS, MGC-803 and MKN-45, normal tissue cell lines and 50 pairs of gastric cancer tissues. Moreover, two cell lines of miR-147b high-expressing used PCR quantitative analysis were selected for transfection experiments. The differentially expressed miR-147b was screened from 3 pairs of samples by miRNA chip. The expression ofmiR-147b was found highly expressed in gastric cancer tissues of 50 pairs of gastric cancer and adjacent tissues. The miR-147b found in diverse range in each of GC cell line. Therefore, two cell lines, BGC-823 and MGC-803, with relatively high expression levels of miR-147b were selected for further analysis and research. Scratch analysis results showed that compared with miR-147b NC, the miR-147b inhibitor group inhibited GC cell growth and reduced cell migration. The early apoptosis of MGC-803, and BGC-823 cells was enhanced by miR-147b inhibitor. miR-147b inhibitor significantly repressed the proliferation of BGC-823 and MGC-803 cells. Our study showed that the high expression of miR-147b is positively correlated with the occurrence and development of gastric cancer.

<italic>MIR-147B</italic> regulated proliferation and apoptosis of gastric cancer cells by targeting <italic>CPEB2</italic> via the PTEN pathway

Balkan Journal of Medical Genetics

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

 regulated proliferation and apoptosis of gastric cancer cells by targeting  via the PTEN pathway

The present study has been performed to illustrate the role and mechanism of microRNA-147b (miR-147b) in the cellular viability and apoptosis of gastric cancer...

MIR-147B regulated proliferation and apoptosis of gastric cancer cells by targeting CPEB2 via the PTEN pathway

Article Category: Original Article

Publié en ligne: 01 mars 2023

Pages: 61 - 70

DOI: https://doi.org/10.2478/bjmg-2022-0007

Mots clés
Gastric cancer, microRNA-147b, apoptosis, cell viability

© 2022 Tao К., Dong J-H., Wang D., Li F., Zhang Z-T., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

MIR-147B regulated proliferation and apoptosis of gastric cancer cells by targeting CPEB2 via the PTEN pathway

Article Category: Original Article

Publié en ligne: 01 mars 2023

Pages: 61 - 70

DOI: https://doi.org/10.2478/bjmg-2022-0007

Mots clésGastric cancer, microRNA-147b, apoptosis, cell viability

© 2022 Tao К., Dong J-H., Wang D., Li F., Zhang Z-T., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
Gastric cancer, microRNA-147b, apoptosis, cell viability