Relationship between chromosomal aberrations and gene expressions in the p53 pathway in chronic lymphocytic leukemia
, , , , , , , , et | 26 août 2020
À propos de cet article
Article Category: Original Article
Publié en ligne: 26 août 2020
Pages: 15 - 24
DOI: https://doi.org/10.2478/bjmg-2020-0007
Mots clés
© 2020 Öztan G, Aktan M, Palanduz S, İşsever H, Öztürk S, Nikerel E, Uçur A, Bağatir G, Bayrak B, Çefle K, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Figure 1
Figure 2
The mean difference values of the patient and control group according to average ᐃCt and fold change.
| Gene | Average Ct | Average ᐃCt | 2-ᐃCt | Fold Change | Fold Regulation | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| CLL | Control | CLL | Control | CLL | Control | CLL/ Control | 95% CI | CLL/ Control | ||
| 26.57 | 25.28 | 7.72 | 7.54 | 0.004695 | 0.005368 | 0.87 | 0.78-0.96 | 0.072729 | -1.14 | |
| 27.85 | 27.91 | 9.01 | 10.17 | 0.001936 | 0.000869 | 2.23 | 1.67-2.79 | <0.001 | 2.23 | |
| 29.00 | 29.41 | 10.17 | 11.67 | 0.000871 | 0.000307 | 2.83 | 2.23-3.43 | <0.001 | 2.83 | |
| 25.61 | 25.87 | 6.78 | 8.13 | 0.009116 | 0.003578 | 2.55 | 2.15-2.95 | <0.001 | 2.55 | |
| 27.35 | 27.58 | 8.52 | 9.84 | 0.002723 | 0.001091 | 2.50 | 2.06-2.94 | <0.001 | 2.50 | |
| 26.92 | 27.96 | 8.09 | 10.22 | 0.003672 | 0.000839 | 4.38 | 3.20-5.56 | <0.001 | 4.38 | |
| 27.59 | 26.82 | 8.75 | 9.08 | 0.002315 | 0.001852 | 1.25 | 0.97-1.53 | 0.066 | 1.25 | |
| 30.50 | 32.77 | 11.67 | 15.02 | 0.000307 | 0.000030 | 10.22 | 7.14-13.3 | <0.001 | 10.22 | |
| 31.37 | 31.58 | 12.54 | 13.83 | 0.000168 | 0.000068 | 2.45 | 1.98-2.92 | <0.001 | 2.45 | |
| 29.45 | 30.42 | 10.62 | 12.68 | 0.000638 | 0.000152 | 4.18 | 3.02-5.34 | <0.001 | 4.18 | |
| 31.44 | 30.88 | 12.60 | 13.14 | 0.000161 | 0.000111 | 1.45 | 1.04-1.86 | 0.012741 | 1.45 | |
| 28.88 | 27.09 | 10.05 | 9.34 | 0.000944 | 0.001541 | 0.61 | 0.53-0.69 | <0.001 | -1.63 | |
| 31.94 | 31.14 | 13.10 | 13.39 | 0.000114 | 0.000093 | 1.22 | 0.90-1.54 | 0.121 | 1.22 | |
| 23.34 | 21.85 | 4.51 | 4.11 | 0.043950 | 0.057965 | 0.76 | 0.66-0.86 | <0.001 | -1.32 | |
| 25.77 | 25.60 | 6.93 | 7.86 | 0.008184 | 0.004312 | 1.90 | 1.56-2.24 | <0.001 | 1.90 | |
| 27.43 | 28.00 | 8.60 | 10.26 | 0.002577 | 0.000818 | 3.15 | 2.32-3.998 | <0.001 | 3.15 | |
| 25.75 | 26.36 | 6.92 | 8.62 | 0.008266 | 0.002545 | 3.25 | 2.64-3.86 | <0.001 | 3.25 | |
| 25.53 | 24.05 | 6.50 | 6.31 | 0.011036 | 0.012615 | 0.87 | 0.78-0.96 | 0.062542 | -1.14 | |
| 26.51 | 27.04 | 7.67 | 9.29 | 0.004894 | 0.001595 | 3.07 | 2.40-3.74 | <0.001 | 3.07 | |
| 25.76 | 26.32 | 6.92 | 8.58 | 0.008229 | 0.002619 | 3.14 | 2.47-3.81 | <0.001 | 3.14 | |
| 32.50 | 30.53 | 13.67 | 12.79 | 0.000077 | 0.000141 | 0.54 | 0.37-0.71 | 0.006057 | -1.84 | |
| 25.94 | 27.94 | 7.11 | 10.20 | 0.007231 | 0.000850 | 8.5' | 5.46-11.56 | <0.001 | 8.51 | |
Patient diagnostic data and FISH results.
| Sex-Age | Prognosis | Rai Stage | Binet Stage | FISH Results | (WBC 109/L) | Hb (g/dL) | Platelet (109/L) | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| del(13q) | del(17p) | +12 | Normal Non-del(13q), del(17p), +12. | ||||||||
| 1 | F-75 | bad | 1 | B | [–] | [+] | [–] | [–] | 12.6 | 12.7 | 166.0 |
| 2 | M-71 | good | 0 | A | [–] | [–] | [–] | [+] | 23.0 | 12.0 | 203.0 |
| 3 | M-57 | good | 0 | A | [–] | [–] | [–] | [+] | 24.8 | 15.1 | 262.0 |
| 4 | M-56 | good | 3 | A | [–] | [–] | [–] | [+] | 71.4 | 13.2 | 49.0 |
| 5 | F-59 | bad | 2 | B | [–] | [+] | [–] | [–] | 7.9 | 10.9 | 284.0 |
| 6 | F-74 | bad | 2 | B | [–] | [+] | [–] | [–] | 108.6 | 10.3 | 190.0 |
| 7 | F-63 | bad | 2 | A | [–] | [+] | [–] | [–] | 35.4 | 13.4 | 185.0 |
| 8 | M-80 | bad | 2 | A | [–] | [+] | [–] | [–] | 3.6 | 12.9 | 104.0 |
| 9 | F-62 | bad | 0 | A | [–] | [–] | [+] | [–] | 190.2 | 12.0 | 192.0 |
| 10 | M-67 | bad | 2 | A | [–] | [–] | [+] | [–] | 142.6 | 11.6 | 81.0 |
| 11 | F-65 | bad | 0 | A | [–] | [–] | [+] | [–] | 5.2 | 12.1 | 144.0 |
| 12 | F-74 | bad | 2 | A | [–] | [–] | [+] | [–] | 179.7 | 11.9 | 321.0 |
| 13 | F-86 | bad | 3 | A | [–] | [–] | [+] | [–] | 213.0 | 8.6 | 113.0 |
| 14 | M-60 | good | 3 | A | [–] | [–] | [–] | [+] | 51.7 | 9.4 | 137.0 |
| 15 | M-46 | good | 2 | A | [–] | [–] | [–] | [+] | 15.0 | 14.4 | 184.0 |
| 16 | M-54 | good | 0 | A | [–] | [–] | [–] | [+] | 41.9 | 13.8 | 352.0 |
| 17 | F-61 | good | 1 | A | [–] | [–] | [–] | [+] | 11.8 | 12.1 | 148.0 |
| 18 | F-67 | good | 0 | A | [–] | [–] | [–] | [+] | 195.3 | 10.8 | 188.0 |
| 19 | F-53 | good | 2 | B | [–] | [–] | [–] | [+] | 39.8 | 11.4 | 135.0 |
| 20 | M-67 | good | 0 | A | [+] | [–] | [–] | [–] | 74.6 | 12.4 | 183.0 |
| 21 | M-62 | good | 2 | A | [+] | [–] | [–] | [–] | 121.4 | 14.5 | 294.0 |
| 22 | M-68 | good | 0 | A | [+] | [–] | [–] | [–] | 37.7 | 13.9 | 197.0 |
| 23 | F-46 | good | 1 | A | [+] | [–] | [–] | [–] | 59.4 | 12.3 | 91.0 |
| 24 | M-58 | good | 2 | B | [+] | [–] | [–] | [–] | 201.4 | 11.1 | 101.0 |
| 25 | M-66 | good | 0 | A | [+] | [–] | [–] | [–] | 43.9 | 12.3 | 88.0 |
| 26 | M-61 | good | 4 | C | [+] | [–] | [–] | [–] | 110.0 | 13.9 | 39.0 |
| 27 | M-51 | good | 2 | A | [+] | [–] | [–] | [–] | 98.5 | 14.3 | 250.0 |
| 28 | F-78 | good | 3 | B | [+] | [–] | [–] | [–] | 13.7 | 11.0 | 324.0 |
| 29 | F-63 | good | 0 | A | [+] | [–] | [–] | [–] | 113.3 | 11.5 | 178.0 |
| 30 | M-64 | good | 0 | A | [+] | [–] | [–] | [–] | 432.0 | 15.3 | 206.0 |