<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The inclusion of exogenous digestive enzymes and probiotics is well established in the aquafeed industry. The mixture of multi-enzymes and probiotics improves the feed utilization and wellbeing of aquatic animals compared to the individual supplementation. Herein, we evaluated the exogenous multi-enzyme mixture (beta-glucanase, cellulase, alpha-amylase, protease, xylanase, and phytase) at 250 mg/kg and multi-species probiotic (<italic>Bacillus subtilis</italic>, <italic>Lactobacillus acidophilus</italic>, <italic>L. delbrueckii</italic>, <italic>L. rhamnosus</italic>, <italic>L. plantarum</italic>, and <italic>Pediococcus acidilactici</italic>; 1 × 10<sup>10</sup> CFU/g for each bacterial strain) at 2 g/kg on the performances of Siberian sturgeon. The final weight, weight gain, SGR, and PER were markedly enhanced while the FCR was reduced in fish fed multi-enzyme and probiotics premix (P&lt;0.05). Multi enzymes and probiotic mixture significantly increased the total body protein content (P˃0.05). Multi enzymes and probiotic mixture also improved the digestibility of crude protein, dry matter, and crude lipids nutrients (P&lt;0.05). The count of goblet cells, microvilli diameter, microvilli length, outer muscle wall diameter, and enterocyte total absorptive surface were markedly increased (P&lt;0.05) by dietary multienzymes and probiotics mixture. The WBCs and neutrophils showed marked improvements (P&lt;0.05). The levels of glucose, triglycerides, blood urea nitrogen, and total bilirubin were markedly higher in fish fed the control than fish fed the multi-enzymes and probiotics mixture (P&lt;0.05). Significantly, Siberian sturgeon-fed dietary multi-enzymes and probiotics had improved lysozyme activity, total immunoglobulin, and total protein in the skin mucus and serum samples (P&lt;0.05). Further, the serum complement C3 and C4 was higher in fish-delivered multi-enzymes and probiotic mixture than in control (P&lt;0.05). In conclusion, dietary probiotics synergistically enhanced the activity of multi-enzymes and resulted in increased feed utilization, nutrient digestibility, and health status of Siberian sturgeon.</p>
</abstract>

The inclusion of exogenous digestive enzymes and probiotics is well established in the aquafeed industry. The mixture of multi-enzymes and probiotics improves the feed utilization and wellbeing of aquatic animals compared to the individual supplementation. Herein, we evaluated the exogenous multi-enzyme mixture (beta-glucanase, cellulase, alpha-amylase, protease, xylanase, and phytase) at 250 mg/kg and multi-species probiotic (Bacillus subtilis, Lactobacillus acidophilus, L. delbrueckii, L. rhamnosus, L. plantarum, and Pediococcus acidilactici; 1 × 1010 CFU/g for each bacterial strain) at 2 g/kg on the performances of Siberian sturgeon. The final weight, weight gain, SGR, and PER were markedly enhanced while the FCR was reduced in fish fed multi-enzyme and probiotics premix (P<0.05). Multi enzymes and probiotic mixture significantly increased the total body protein content (P˃0.05). Multi enzymes and probiotic mixture also improved the digestibility of crude protein, dry matter, and crude lipids nutrients (P<0.05). The count of goblet cells, microvilli diameter, microvilli length, outer muscle wall diameter, and enterocyte total absorptive surface were markedly increased (P<0.05) by dietary multienzymes and probiotics mixture. The WBCs and neutrophils showed marked improvements (P<0.05). The levels of glucose, triglycerides, blood urea nitrogen, and total bilirubin were markedly higher in fish fed the control than fish fed the multi-enzymes and probiotics mixture (P<0.05). Significantly, Siberian sturgeon-fed dietary multi-enzymes and probiotics had improved lysozyme activity, total immunoglobulin, and total protein in the skin mucus and serum samples (P<0.05). Further, the serum complement C3 and C4 was higher in fish-delivered multi-enzymes and probiotic mixture than in control (P<0.05). In conclusion, dietary probiotics synergistically enhanced the activity of multi-enzymes and resulted in increased feed utilization, nutrient digestibility, and health status of Siberian sturgeon.

The multi-enzymes and probiotics mixture improves the growth performance, digestibility, intestinal health, and immune response of Siberian sturgeon (<italic>Acipenser baerii</italic>)

Department of Fisheries, Science and Research Branch

Department of Animal Production, Faculty of Agriculture

Annals of Animal Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

The multi-enzymes and probiotics mixture improves the growth performance, digestibility, intestinal health, and immune response of Siberian sturgeon ()

The inclusion of exogenous digestive enzymes and probiotics is well established in the aquafeed industry. The mixture of multi-enzymes and probiotics improves...

The multi-enzymes and probiotics mixture improves the growth performance, digestibility, intestinal health, and immune response of Siberian sturgeon (Acipenser baerii)

Publié en ligne: 19 juil. 2022

Pages: 1063 - 1072

Reçu: 27 sept. 2021

Accepté: 07 déc. 2021

DOI: https://doi.org/10.2478/aoas-2022-0006

Mots clés
aquaculture, exogenous enzymes, probiotics, digestibility, growth promoter, nutrient digestibility

© 2022 Seyed Pezhman Hosseini Shekarabi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

The multi-enzymes and probiotics mixture improves the growth performance, digestibility, intestinal health, and immune response of Siberian sturgeon (Acipenser baerii)

Publié en ligne: 19 juil. 2022

Pages: 1063 - 1072

Reçu: 27 sept. 2021

Accepté: 07 déc. 2021

DOI: https://doi.org/10.2478/aoas-2022-0006

Mots clésaquaculture, exogenous enzymes, probiotics, digestibility, growth promoter, nutrient digestibility

© 2022 Seyed Pezhman Hosseini Shekarabi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Mots clés
aquaculture, exogenous enzymes, probiotics, digestibility, growth promoter, nutrient digestibility