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Archives of Industrial Hygiene and Toxicology
Édition 74 (2023): Edition 1 (March 2023)
Accès libre
Different damaging effects of volatile anaesthetics alone or in combination with 1 and 2 Gy gamma-irradiation
in vivo
on mouse liver DNA: a preliminary study
Vesna Benković
Vesna Benković
,
Mirta Milić
Mirta Milić
,
Nada Oršolić
Nada Oršolić
,
Anica Horvat Knežević
Anica Horvat Knežević
,
Gordana Brozović
Gordana Brozović
et
Nikola Borojević
Nikola Borojević
| 04 avr. 2023
Archives of Industrial Hygiene and Toxicology
Édition 74 (2023): Edition 1 (March 2023)
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Article Category:
Original article
Publié en ligne:
04 avr. 2023
Pages:
22 - 33
Reçu:
01 nov. 2022
Accepté:
01 mars 2023
DOI:
https://doi.org/10.2478/aiht-2023-74-3692
© 2023 Vesna Benković et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Figure 1
Scheme of the experiment. Male Swiss albino mice (n=240) were divided into 12 groups (control; 1 or 2 Gy irradiated; exposed to only halothane, sevoflurane or isoflurane; exposed to a combination of 1 or 2 Gy and either halothane, sevoflurane or isoflurane) and further each group was divided into 4 subgroups (according to the liver sampling time after the exposure- 0, 2, 6 and 24 hours) with 5 animals in each subgroup. Alkaline Comet assay was used for DNA damage and cellular repair index assessment
Figure 2a
Tail length values in liver cells of non-irradiated male Swiss albino mice anaesthetised solely with halothane (H), sevoflurane (S) or isoflurane (I). Samples taken immediately after (0 h), 2, 6 or 24 h after treatment were analysed from five animals per group for 200 comets. C-control. Samples were compared to control 0 h (C 0h), statistical differences (*) were analysed with the Mann-Whitney U test. The values represented here are mean (square), median (triangle), and standard deviation (SD, range)
Figure 2b
Tail intensity values in liver cells of non-irradiated male Swiss albino mice anaesthetised solely with halothane (H), sevoflurane (S) or isoflurane (I). Samples taken immediately after (0 h), 2, 6 or 24h after treatment were analysed from five animals per group for 200 comets. C-control. Samples were compared to control 0 h (C 0h), statistical differences (*) were analysed with the Mann-Whitney U test. The values represented here are mean (square), median (triangle), and standard deviation (SD, range)
Figure 2c
Tail moment values in liver cells of non-irradiated male Swiss albino mice anaesthetised solely with halothane (H), sevoflurane (S) or isoflurane (I). Samples taken immediately after (0 h), 2, 6 or 24 h after treatment were analysed from five animals per group for 200 comets. C-control. Samples were compared to control 0 h (C 0h), statistical differences (*) were analysed with the Mann-Whitney U test. The values represented here are mean (square), median (triangle), and standard deviation (SD, range)
Figure 3a
Tail length values in liver cells of 1 Gy irradiated male Swiss albino mice previously anaesthetised with halothane (H), sevoflurane (S) or isoflurane (I). Samples taken immediately after (0 h), 2, 6 or 24 h after treatment were analysed from five animals per group for 200 comets. Samples were compared to 1 Gy 0 h, statistical differences (*) were analysed with the Mann-Whitney U test. The values represented here are mean (square), median (triangle), and standard deviation (SD, range)
Figure 3b
Tail intensity values in liver cells of 1 Gy irradiated male Swiss albino mice previously anaesthetised with halothane (H), sevoflurane (S) or isoflurane (I). Samples taken immediately after (0 h), 2, 6 or 24 h after treatment were analysed from five animals per group for 200 comets. Samples were compared to 1 Gy 0 h, statistical differences (*) were analysed with the Mann-Whitney U test. The values represented here are mean (square), median (triangle), and standard deviation (SD, range)
Figure 3c
Tail moment values in liver cells of 1 Gy irradiated male Swiss albino mice previously anaesthetised with halothane (H), sevoflurane (S) or isoflurane (I). Samples taken immediately after (0 h), 2, 6 or 24 h after treatment were analysed from five animals per group for 200 comets. Samples were compared to 1 Gy 0 h, statistical differences (*) were analysed with the Mann-Whitney U test. The values represented here are mean (square), median (triangle), and standard deviation (SD, range)
Figure 4a
Tail length values in liver cells of 2 Gy irradiated male Swiss albino mice previously anaesthetised with halothane (H), sevoflurane (S) or isoflurane (I). Samples taken immediately after (0 h), 2, 6 or 24 h after treatment were analysed from five animals per group for 200 comets. Samples were compared to 2 Gy 0 h, statistical differences (*) were analysed with the Mann-Whitney U test. The values represented here are mean (square), median (triangle), and standard deviation (SD, range).
Figure 4b
Tail intensity values in liver cells of 2 Gy irradiated male Swiss albino mice previously anaesthetised with halothane (H), sevoflurane (S) or isoflurane (I). Samples taken immediately after (0 h), 2, 6 or 24 h after treatment were analysed from five animals per group for 200 comets. Samples were compared to 2 Gy 0 h, statistical differences (*) were analysed with the Mann-Whitney U test. The values represented here are mean (square), median (triangle), and standard deviation (SD, range)
Figure 4c
Tail moment values in liver cells of 2 Gy irradiated male Swiss albino mice previously anaesthetised with halothane (H), sevoflurane (S) or isoflurane (I). Samples taken immediately after (0 h), 2, 6 or 24 h after treatment were analysed from five animals per group for 200 comets. Samples were compared to 2 Gy 0 h, statistical differences (*) were analysed with the Mann-Whitney U test. The values represented here are mean (square), median (triangle) and standard deviation (SD, range)
Figure 5
CRI index (percentage of repair) for tail length (TL) and tail intensity (TI) parameter measured in liver cells of mice for 24 hours. 0 h-immediately after, 2 h, 6 h and 24 h after combined exposure to anaesthetics and: 1 Gy (A, B) or 2 Gy (C, D) γ-irradiation (60Co). H-halothane, S-sevoflurane, I-isoflurane
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