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Redox and essential metal status in the brain of Wistar rats acutely exposed to a cadmium and lead mixture

Dragana Javorac
Dragana Javorac
, 
Aleksandra Buha Đorđević
Aleksandra Buha Đorđević
, 
Milena Anđelković
Milena Anđelković
, 
Simona Tatović
Simona Tatović
, 
Katarina Baralić
Katarina Baralić
, 
Evica Antonijević
Evica Antonijević
, 
Jelena Kotur-Stevuljević
Jelena Kotur-Stevuljević
, 
Danijela Đukić-Ćosić
Danijela Đukić-Ćosić
, 
Biljana Antonijević
Biljana Antonijević
 et 
Zorica Bulat
Zorica Bulat
   | 06 oct. 2020
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Article Category: Original article
Publié en ligne: 06 oct. 2020
Pages: 197 - 204
Reçu: 01 avr. 2020
Accepté: 01 sept. 2020
DOI: https://doi.org/10.2478/aiht-2020-71-3425
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© 2020 Dragana Javorac et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Brain Pb levels in Wistar rats after acute exposure to Pb alone and in combination with Cd (N=6–8 per group). *** P<0.001 compared to control (one-way ANOVA followed by LSD). Pb 150 – the group treated with Pb in the dose of 150 mg/kg bw; Cd15+Pb150 – the group treated with a combination of 150 mg/kg Pb and 15 mg/kg of Cd. Boxes represent interquartile ranges (25–75th percentile), the line within the box median values, and the ends of the whiskers minimum and maximum values within the group
Brain Pb levels in Wistar rats after acute exposure to Pb alone and in combination with Cd (N=6–8 per group). *** P<0.001 compared to control (one-way ANOVA followed by LSD). Pb 150 – the group treated with Pb in the dose of 150 mg/kg bw; Cd15+Pb150 – the group treated with a combination of 150 mg/kg Pb and 15 mg/kg of Cd. Boxes represent interquartile ranges (25–75th percentile), the line within the box median values, and the ends of the whiskers minimum and maximum values within the group

Figure 2

Brain Cd levels in Wistar rats after acute exposure to Cd alone and in combination with Pb (N=6–8 per group). * P<0.05, ** P<0.01, *** P<0.001 compared to control; † P<0.05, †† P<0.01 compared to Cd15; ‡ p<0.05, compared to Cd30 (one-way ANOVA followed by LSD). Cd15 – the group treated with Cd in the dose of 15 mg/kg bw; Cd30 – the group treated with Cd in the dose of 30 mg/kg bw; Cd15+Pb150 – the group treated with a combination of 150 mg/kg Pb and 15 mg/kg Cd. Boxes represent interquartile ranges (25–75th percentile), the line within the box median value, and the ends of the whiskers minimum and maximum values within the group
Brain Cd levels in Wistar rats after acute exposure to Cd alone and in combination with Pb (N=6–8 per group). * P<0.05, ** P<0.01, *** P<0.001 compared to control; † P<0.05, †† P<0.01 compared to Cd15; ‡ p<0.05, compared to Cd30 (one-way ANOVA followed by LSD). Cd15 – the group treated with Cd in the dose of 15 mg/kg bw; Cd30 – the group treated with Cd in the dose of 30 mg/kg bw; Cd15+Pb150 – the group treated with a combination of 150 mg/kg Pb and 15 mg/kg Cd. Boxes represent interquartile ranges (25–75th percentile), the line within the box median value, and the ends of the whiskers minimum and maximum values within the group

Brain Zn, Cu, Mn, and Fe levels in Wistar rats after acute exposure to Pb and/or Cd (N=6–8 per group)

Groups
Essential metals Control (μg/g) Cd15 (μg/g) Cd30 (μg/g) Pb150 (μg/g) Cd15+Pb150 (μg/g)
Zn 11.1210.69–15.10 12.3511.39–13.71 12.6812.12–15.66 12.7410.87–14.89 14.8411.16–15.40
Cu 1.99±0.41 2.24±0.27 2.17±0.23 2.26±0.26 1.88±0.22
Mn 1.93±0.44 1.38±0.24 1.72±0.34 1.65±0.40 1.90±0.51
Fe 22.4215.52–26.35 18.1216.08–30.81 19.3115.85–33.61 21.1919.12–22.86 16.8713.44–24.93

Effects of acute Pb and/or Cd treatment on oxidative stress parameters in the brain of Wistar rats (N=6–8 per group)

Groups
Parameter Control Cd15 Cd30 Pb150 Cd15+Pb150
TBARS (μmol/mg protein) 161.36±47.74 175.25±43.23 309.64±106.78** † 230.95±47.82 390.57±95.15*** †† §
AOPP (μmol/min/g protein) 27.57±6.28 24.76±4.90 27.68±6.43 25.4±6.18 24.83±8.83
O2·− (μmol/min/g protein) 91.74±6.65 99.78±17.30 91.38±18.94 95.13±11.82 90.24±7.92
SOD (U/g) 57.35±10.63 47.52±5.03 57.73±18.82 58.29±5.87 49.32±10.92
SH(mmol/g protein) 0.14±0.03 0.15±0.02 0.13±0.02 0.15±0.02 0.12±0.02
TAS(μmol Trolox Equiv. /g protein) 130.00 87.70–188.28 268.66* 232.28–368.51 300.07*** 274.71–346.72 222.61** ‡‡ 108.48–336.72 264.91** 221.20–274.67
TOS(μmol H2O2 Equiv. /g protein) 2.36±0.68 2.46±0.53 2.05±0.54 2.77±0.65 2.89±1.89
OSI 0.0162±0.0078 0.0106±0.0049* 0.0068±0.0016** 0.0149±0.0087‡ 0.0099±0.0037*
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