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Figure 1.
Schematic presentation of the analysis of G418 influence on cells’ metabolic activity
Figure 2.
Viability of normal human skin cells, cultured in M254 culture medium, after cell culture stimulation with different concentrations of geneticin for 24 hours (A) and 72 hours (B)
Figure 3.
The viability of primary keratinocytes measured 24 h (A) and 72 h (B) after cell culture stimulation with increasing concentrations of geneticin, measured by the means of MTT assay. All absorbance values were normalized versus control keratinocytes obtained after 24 hours of cell culture
Figure 4.
The viability of primary fibroblasts measured 24 h (A) and 72 h (B) after cell culture stimulation with increasing concentrations of geneticin measured by the means of MTT assay. All absorbance values were normalized versus control fibroblasts obtained after 24 hours of cell culture
Figure 5.
The proliferation rates of primary fibroblasts cultured in different media supplemented with geneticin (0.05–1 mg/ml) measured using the BrdU assay (A). The quantitative analysis of apoptosis and necrosis of fibroblasts treated with geneticin with staurosporine/etoposide as positive control samples (K(+)), respectively (B)
Figure 6.
Immunofluorescence staining of melanocytes, keratinocytes and fibroblasts cultured in medium M254 for 72 hours after 0.05 mg/mL geneticin treatment. The nuclei of the cells were stained blue with DAPI. TYR and NG2 are markers of melanocytes, while COL1 and KRT14 are markers of fibroblasts and keratinocytes, respectively
Figure 7.
The MTT results of the influence of geneticin on the primary human metastatic melanoma cell lines (WCCm1, WCCm7, WCCd9) and commercial cell lines of metastatic melanoma (A375, G-361 and MeWo) observed 24 hours (A) and 72 hours (B) after cell stimulation in M254 culture medium
Figure 8.
Flow of information through the selected literature about concentrations of geneticin used for culture of pure melanocytes
Percentage of particular cells in co-cultures of melanocytes, fibroblasts, and keratinocytes in different culture media for 72 hours following treatment with 0.05 or 0.1 mg/mL geneticin