Role of geneticin in isolation and culturing of skin melanocytes and melanoma cells
, , , , , , , , y | 14 sept 2023
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Article Category: Original Study
Publicado en línea: 14 sept 2023
Páginas: 72 - 81
Recibido: 12 mar 2023
Aceptado: 06 jul 2023
DOI: https://doi.org/10.2478/ahem-2023-0014
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© 2023 Aneta Ścieżyńska et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
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Percentage of particular cells in co-cultures of melanocytes, fibroblasts, and keratinocytes in different culture media for 72 hours following treatment with 0.05 or 0.1 mg/mL geneticin
| Culture media | Cell type | Control | Geneticin (0.05 mg/mL) | Geneticin (0.1 mg/mL) |
|---|---|---|---|---|
| DMEM + 10% FBS | Melanocytes | 15% | 29% | 37% |
| Fibroblasts | 67% | 66% | 60% | |
| Keratinocytes | 18% | 5% | 3% | |
| RPMI + 10% FBS | Melanocytes | 26% | 40% | 66% |
| Fibroblasts | 60% | 55% | 30% | |
| Keratinocytes | 14% | 5% | 4% | |
| M254 | Melanocytes | 27% | 97% | 98% |
| Fibroblasts | 40% | 2% | 1% | |
| Keratinocytes | 33% | 1% | 1% |