Evaluation of antitumoral effect of mistletoe fruit extract on Ehrlich ascites tumor cells with muse cell analyzer and argyrophilic nucleolar organizer region staining method
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Fig. 1
Experimental processes in this study
Fig. 2
The raw graphics taken from the Muse® Cell Analyzer. A: Muse® Annexin V and Dead Cell Assay, B: Muse® Cell Cycle Assay, C: Muse® Mitopotential Assay
Fig. 3
Data of the annexin V & dead cell assay at the end of 24, 48, and 72 hours of incubation. p<0.05 was considered statistically significant. Data are expressed as mean ± SEM (standard error of mean). There is no statistically significant difference between the groups containing the same letter (p> 0.05). A: Live cell count (%), B: Early apoptotic cell count (%), C: Late apoptotic cell count (%), D: Total apoptotic cell count (%). 5-FU: 5-fluorouracil; 100, 200, 400, 800 μg/ml: Mistletoe groups
Fig. 4
Data of the cell cycle assay at the end of 24, 48, and 72 hours of incubation. p <0.05 was considered statistically significant. Data are expressed as mean ± SEM. There is no statistically significant difference between groups containing the same letter (p> 0.05). A: Cell cycle distribution-G0 / G1 stage (%), B: Cell cycle distribution-S stage (%), C: Cell cycle distribution-G2 / M stage (%). 5-FU: 5-fluorouracil; 100, 200, 400, 800 μg/ml: Mistletoe groups
Fig. 5
Data of the mitopotential assay at the end of 24, 48, and 72 hours of incubation. p <0.05 was considered statistically significant. Data are expressed as mean ± SEM. There is no statistically significant difference between the groups containing the same letter (p> 0.05). A: Live depolarized cell count (%), B: Dead depolarized cell count (%), C: Total depolarized cell count (%). 5-FU: 5-fluorouracil; 100, 200, 400, 800 μg/ml: Mistletoe groups
Fig. 6
Image examples of the AgNOR staining cells. A. Control group, B. 5-FU group, C. 100 μg / ml mistletoe group, D. 200 μg/ml mistletoe group, E. 400 μg / ml mistletoe group, F. 800 μg/ml mistletoe group
Mean AgNOR number after 24, 48, and 72 hours of incubation
Hours/Groups
Control
5-FU
100 μg/ml
200 μg/ml
400 μg/ml
800 μg/ml
p
24 hours
3.54±1.26a
2.82±1.06b
2.98±1.16ab
1.74±0.77c
1.74±1.00c
1.80±1.19c
<0.001
48 hours
3.68±1.55a
3.16±1.07a
2.40±0.78b
2.12±1.25b
1.96±0.75b
1.28±0.53c
<0.001
72 hours
1.52±0.64a
2.20±1.08bc
2.26±1.24c
2.20±1.01bc
1.68±0.76ab
1.74±0.85abc
<0.001
Total phenolic and flavonoid contents of mistletoe fruit
Total phenol (mgGAE/gextract)
Total flavonoid (mgCAE/gextract)
Mistletoe fruit
6.24±2.26
4.82±1.36
TAA / NA value at the end of 24, 48, and 72 hours of incubation