Atomic force microscopy and scanning electron microscopy as alternative methods of early identification of pathogens causing catheter-related bloodstream infections of patients in ICU Article Category: Original Study Publié en ligne: 13 mai 2022 Pages: 157 - 164 Reçu: 16 janv. 2021 Accepté: 10 déc. 2021 © 2022 Anna Kluzik et al., published by Sciendo This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Fig. 1 The surface area of brand new catheters with visible grooves (a) AFM image, scan area – 10 ×10 μm, colour scale 500 nm. (b) SEM image, horizontal side of the image – 8.3 μm Fig. 2 An AFM image (a) and SEM images (b, c) of the catheter surface – Staphylococcus spp. covered with biofilm. The scales are shown in the pictures Fig. 3 SEM images of the catheter: (a) formation of biofilm by connecting bacteria using exopolysaccharide skeleton (b) Staphylococcus spp. colonies covered with biofilm Fig. 4 SEM images of the catheter: (a) Typical colonies of bacteria from the Staphylococcus group. (b) No biofilm was detected at high magnification Fig. 5 An SEM image of the catheter. Bacteria can be seen in the morphology of Bacillus spp Fig. 6 An SEM image of the catheter: Morganella morganii, Acinetobacter baumannii, Staphylococcus epidermidis in a catheter tip culture, biofilm Fig. 7 An SEM image of the catheter: Staphylococcus haemoliticus in a catheter tip culture, biofilm Fig. 8 An SEM image of the catheter: Morganella morganii, Acinetobacter baumannii, Bacillus, Staphylococcus epidermidis in a catheter tip culture, biofilm