<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Over the past decades, different mycobacteriosis agents have become important fish pathogens. The present study describes a different mycobacteriosis in farmed sea bream (<italic>Sparus aurata</italic>) caused by <italic>Mycobacterium frederiksbergense</italic> in Turkey. Affected 15 fish, weighing 15 to 20 g, showed lethargy, stunted growth, pale skin, dorsal fin necrosis, and a significant level of mortality (40 %) in fish stocks. Internally, no multifocal white-colored granulomas in the visceral organs were observed. Inoculation of the visceral organs onto Löwenstein-Jensen medium and Tryptic Soy Agar (1.5% NaCl) slants produced only fast-growing (2-3 weeks), orange to yellow-colored, photochromogenic acid-fast colonies. Ziehl-Nielsen positive bacterial isolates were identified using a commercially available line probe assay (Genotype Mycobacterium CM/AS assay) and with 16S rRNA gene sequencing analysis based on 16S rRNA gene sequencing, fifteen isolates were identified as <italic>Mycobacterium frederiksbergense</italic>. Histopathologically, epithelioid cell granulomas were not observed in any vicseral organs but acid-fast bacteria were detected in the liver, kidney, spleen, and heart tissue. This study shows that asystemic mycobacteriosis is observed in sea bream with high mortality.</p></abstract>

Over the past decades, different mycobacteriosis agents have become important fish pathogens. The present study describes a different mycobacteriosis in farmed sea bream (Sparus aurata) caused by Mycobacterium frederiksbergense in Turkey. Affected 15 fish, weighing 15 to 20 g, showed lethargy, stunted growth, pale skin, dorsal fin necrosis, and a significant level of mortality (40 %) in fish stocks. Internally, no multifocal white-colored granulomas in the visceral organs were observed. Inoculation of the visceral organs onto Löwenstein-Jensen medium and Tryptic Soy Agar (1.5% NaCl) slants produced only fast-growing (2-3 weeks), orange to yellow-colored, photochromogenic acid-fast colonies. Ziehl-Nielsen positive bacterial isolates were identified using a commercially available line probe assay (Genotype Mycobacterium CM/AS assay) and with 16S rRNA gene sequencing analysis based on 16S rRNA gene sequencing, fifteen isolates were identified as Mycobacterium frederiksbergense. Histopathologically, epithelioid cell granulomas were not observed in any vicseral organs but acid-fast bacteria were detected in the liver, kidney, spleen, and heart tissue. This study shows that asystemic mycobacteriosis is observed in sea bream with high mortality.

Mycobacteriosis in Farmed Sea Bream (<italic>Sparus aurata</italic>) Caused By <italic>Mycobacterium frederiksbergense</italic> in Turkey

Department of Fish Disease, Faculty of Aquatic Sciences

Department of Medical Microbiology, Istanbul Faculty of Medicine

Acta Veterinaria

This work is licensed under the Creative Commons Attribution 4.0 International License.

Mycobacteriosis in Farmed Sea Bream () Caused By  in Turkey

Over the past decades, different mycobacteriosis agents have become important fish pathogens. The present study describes a different mycobacteriosis in farmed...

Mycobacteriosis in Farmed Sea Bream (Sparus aurata) Caused By Mycobacterium frederiksbergense in Turkey

Publié en ligne: 01 janv. 2019

Pages: 391 - 400

Reçu: 10 sept. 2018

Accepté: 19 nov. 2018

DOI: https://doi.org/10.2478/acve-2018-0032

Mots clés
sea bream, Genotype Mycobacterium CM/AS assay, 16S rRNA gene

© 2018 Urku Cigdem et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Mycobacteriosis in Farmed Sea Bream (Sparus aurata) Caused By Mycobacterium frederiksbergense in Turkey

Publié en ligne: 01 janv. 2019

Pages: 391 - 400

Reçu: 10 sept. 2018

Accepté: 19 nov. 2018

DOI: https://doi.org/10.2478/acve-2018-0032

Mots cléssea bream, Genotype Mycobacterium CM/AS assay, 16S rRNA gene

© 2018 Urku Cigdem et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Mots clés
sea bream, Genotype Mycobacterium CM/AS assay, 16S rRNA gene