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Volume 72 (2022): Edition 2 (June 2022)

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Volume 71 (2021): Edition 4 (December 2021)

Volume 71 (2021): Edition 3 (September 2021)

Volume 71 (2021): Edition 2 (June 2021)

Volume 71 (2021): Edition 1 (March 2021)

Volume 70 (2020): Edition 4 (December 2020)

Volume 70 (2020): Edition 3 (September 2020)

Volume 70 (2020): Edition 2 (June 2020)

Volume 70 (2020): Edition 1 (March 2020)

Volume 69 (2019): Edition 4 (December 2019)

Volume 69 (2019): Edition 3 (September 2019)

Volume 69 (2019): Edition 2 (June 2019)

Volume 69 (2019): Edition 1 (March 2019)

Volume 68 (2018): Edition 4 (December 2018)

Volume 68 (2018): Edition 3 (September 2018)

Volume 68 (2018): Edition 2 (June 2018)

Volume 68 (2018): Edition 1 (March 2018)

Volume 67 (2017): Edition 4 (December 2017)

Volume 67 (2017): Edition 3 (September 2017)

Volume 67 (2017): Edition 2 (June 2017)

Volume 67 (2017): Edition 1 (March 2017)

Volume 66 (2016): Edition 4 (December 2016)

Volume 66 (2016): Edition 3 (September 2016)

Volume 66 (2016): Edition 2 (June 2016)

Volume 66 (2016): Edition 1 (March 2016)

Volume 65 (2015): Edition 4 (December 2015)

Volume 65 (2015): Edition 3 (September 2015)

Volume 65 (2015): Edition 2 (June 2015)

Volume 65 (2015): Edition 1 (March 2015)

Volume 64 (2014): Edition 4 (December 2014)

Volume 64 (2014): Edition 3 (September 2014)

Volume 64 (2014): Edition 2 (June 2014)

Volume 64 (2014): Edition 1 (March 2014)

Détails du magazine
Format
Magazine
eISSN
1820-7448
Première publication
25 Mar 2014
Période de publication
4 fois par an
Langues
Anglais

Chercher

Volume 68 (2018): Edition 4 (December 2018)

Détails du magazine
Format
Magazine
eISSN
1820-7448
Première publication
25 Mar 2014
Période de publication
4 fois par an
Langues
Anglais

Chercher

10 Articles
access type Accès libre

Production of Traditional Meat Products in Small and Micro Establishments in Serbia: Current Status and Future Perspectives

Publié en ligne: 01 Jan 2019
Pages: 373 - 390

Résumé

Abstract

The production of traditional meat products has a considerable potential in the Republic of Serbia, particularly in small and micro (household) establishments. Among a large number of traditional meat products, dry fermented sausages and dried meat products are the most important and commonly appreciated by consumers. There is, however, a need for a better standardization of the production in this meat sector, and also implementation of necessary food hygiene rules and HACCP principles according to hygiene regulations. There are provisions in the Food Safety Law, stating the principles of flexibility, which would allow for traditional meat producers and their associations to apply for derogations in food hygiene regulations. This would enable traditional small and micro food business operators to better use their resources, relax administrative burden and use traditional production methods in the most appropriate manner, always respecting hygiene and safety principals. This review paper analyses the current status of traditional meat production in small and micro establishments in Serbia, emphasizing a need for further improvements in food safety management and standardization.

Mots clés

  • food
  • traditional
  • hygiene
  • safety
  • management
  • flexibility
access type Accès libre

Mycobacteriosis in Farmed Sea Bream (Sparus aurata) Caused By Mycobacterium frederiksbergense in Turkey

Publié en ligne: 01 Jan 2019
Pages: 391 - 400

Résumé

Abstract

Over the past decades, different mycobacteriosis agents have become important fish pathogens. The present study describes a different mycobacteriosis in farmed sea bream (Sparus aurata) caused by Mycobacterium frederiksbergense in Turkey. Affected 15 fish, weighing 15 to 20 g, showed lethargy, stunted growth, pale skin, dorsal fin necrosis, and a significant level of mortality (40 %) in fish stocks. Internally, no multifocal white-colored granulomas in the visceral organs were observed. Inoculation of the visceral organs onto Löwenstein-Jensen medium and Tryptic Soy Agar (1.5% NaCl) slants produced only fast-growing (2-3 weeks), orange to yellow-colored, photochromogenic acid-fast colonies. Ziehl-Nielsen positive bacterial isolates were identified using a commercially available line probe assay (Genotype Mycobacterium CM/AS assay) and with 16S rRNA gene sequencing analysis based on 16S rRNA gene sequencing, fifteen isolates were identified as Mycobacterium frederiksbergense. Histopathologically, epithelioid cell granulomas were not observed in any vicseral organs but acid-fast bacteria were detected in the liver, kidney, spleen, and heart tissue. This study shows that asystemic mycobacteriosis is observed in sea bream with high mortality.

Mots clés

  • sea bream
  • Genotype Mycobacterium CM/AS assay
  • 16S rRNA gene
access type Accès libre

Application of the 3R Concept in the Production of European Antiviperinum on Horses – Multisite, Low Volumes Immunization Protocol and Elisa

Publié en ligne: 01 Jan 2019
Pages: 401 - 419

Résumé

Abstract

During time, both professionals and general public became aware of the importance of animal welfare. This term not only covers endangered wild animal species, animals used in food industry, pets and experimental animals, but also animals used in production of biologics. The implementation of the 3R concept (Replacement, Reduction and Refinement) is especially important in this type of production. In this article, we describe for the first time the low dose, low volume and multi-site immunization protocol, as well as appropriate ELISA we developed for production of European anti-viper (V. ammodytes, long horned) antivenom in horses, which can help to significantly improve the welfare of the used animals.

Mots clés

  • adjuvant
  • animal welfare
  • antivenom production
  • European vipers
  • horses
  • immunization schedule
access type Accès libre

Relationships Between Concentrations of Biological Variables in Eye Fluids and Blood After Exercise in Lidia Cattle

Publié en ligne: 01 Jan 2019
Pages: 420 - 433

Résumé

Abstract

Lidia cattle are a heterogeneous Iberian cattle population known for its natural aggressiveness and resistance to traditional handling procedures making in vivo blood sampling and biological fluid collections extremely difficult. Blood variables are influenced by physical exertion and stressful situations; consequently, post-mortem blood analysis does not reflect basal concentrations for this species. Nevertheless, ocular fluids (aqueous and vitreous humour) maintain their stable composition after death and maybe could be used to estimate ante-mortem blood concentrations. So, 15 bulls which had fought (for 15-20 minutes) and, subsequently after death, blood, aqueous and vitreous humour were sampled. Total protein, albumin, triglycerides, uric acid, urea, AST, ALT, GGT, AP, CK, LDH, cholesterol, creatinine, glucose and lactate were measured. Statistical analysis and correlation coefficients between the three fluids were carried out. All variables showed high plasma concentrations of glucose, uric acid, LDH and CK, compared with normal bovine concentrations. Apart from urea, all plasma concentrations were greater than those found in ocular fluids. The measured enzymes activities were higher in the vitreous than in the aqueous humour, but only marked differences in uric acid, lactate, AP and AST were found. There was a significant correlation between creatinine in the plasma and aqueous humour, and between albumin and GGT in the plasma and vitreous humour. Glucose, creatinine and urea exhibit a high correlation between ocular fluids. All plasma concentrations were clearly modified, however ocular fluids do not seem affected, thus establishing important correlations between the blood and intraocular fluids.

Mots clés

  • aqueous humor
  • vitreous humor
  • biochemical variables
  • intraocular fluids
  • blood
  • metabolism
  • Lidia cattle
access type Accès libre

Histopathological Characteristics and Expression of CDV-NP Antigen in the Brain of Serologically Positive Spontaneously Infected Red Foxes (Vulpes Vulpes) In Western Serbia

Publié en ligne: 01 Jan 2019
Pages: 434 - 444

Résumé

Abstract

Canine distemper virus (CDV) is a worldwide distributed RNA virus that can cause severe disease in carnivore and non-carnivore species. Red foxes are highly susceptible and may act as a reservoir of the virus. As in other wild species, distemper in red foxes can manifest as acute, systemic and chronic nervous form. In the present study, we detected antibodies against CDV among red foxes in Western Serbia, and analyzed histopathologically and immunohistochemically for CDV nuclear protein antigen (CDV-NP) brain samples derived from seropositive animals. Seroprevalence of CDV antibodies was 36.8%. Histopathological changes included gliosis, neuronal degeneration, satellitosis, mononuclear inflammation, demyelination and presence of inclusion bodies. Immunostaining showed a diffuse presence of CDV-NP antigen, mainly in the cytoplasm of astrocytes and neurons. Results of this work contribute to the opinion that red foxes act as a potential reservoir of CDV and underline the importance of routine vaccination of dogs that could come in close contact with these animals. Potential active surveillance program would give a better insight in the degree of CDV infection in wildlife.

Mots clés

  • brain
  • canine distemper virus
  • histopathology
  • immunohistochemistry
  • red fox
access type Accès libre

Antihelminic Activity of Carvacrol, Thymol, Cinnamaldehyde and P-Cymen Against the Free-Living Nematode Caenorhabditis elegans and Rat Pinworm Syphacia muris

Publié en ligne: 01 Jan 2019
Pages: 445 - 456

Résumé

Abstract

In the present study we tested the dose andh time dependence of the antinematodal effects of carvacrol and tyhmol on Caenorabditis elegans, and the efficacy of carvacrol, thymol, p-cymene and cinnamaldehyde,which were administrated in the drinking water of rats naturally infected with the pinworm Syphacia muris. The control treatment of the infected rats was carried out with piperazine. Thymol caused a dose and time-dependent mortality in adult C. elegans. The value of the Median Lethal Concentration (LC50) of thymol was 117.9nM after 24h and 62.89 nM after 48h of exposure. Carvacrol exhibited a higher antinematodal efficiency than thymol. The LC50 of carvacrol, after 24 hours of exposure, was 53.03 nM, while after 48 hours it was 33.83 nM. On the other hand, piperazine showed an extremely high efficacy against S. muris infection in rats. Piperazine, at a dose of 625 mg/kg bw, administered in drinking water continuously for 10 days, eliminates the infection completely. However, none of the investigated active ingredients of essential oils were effective against S. muris. The reason for the lack of efficiency may be due to their pharmacokinetic properties. A relatively low amount of, orally administered, active ingredients of essential oils reaches the distal segments of the gastrointestinal tract, where S. muris inhabits the gut (colon and cecum). The obtained results, on C. elegans, indicate a clear dose and time-dependent antinematodal effect of thymol and carvacrol. However, for clinical application, it is necessary to examine the efficacy of microencapsulated formulations with a controlled release of active ingredients of essential oils in certain parts of the gastrointestinal tract.

Mots clés

  • carvacrol
  • thymol
  • piperazine
access type Accès libre

In Situ Programme for the Conservation of the Autochthonous Lipe Type of Zackel Sheep

Publié en ligne: 01 Jan 2019
Pages: 457 - 473

Résumé

Abstract

The aim of this study was to emphasize the importance of conservation of the Lipe sheep, as a local endangered type of Zackel breed, and to perform a comprehensive phenotypic characterization in order to ensure a better use and preservation of this genetic resource. In addition, we compared the results of a detailed morphometric characterization of the modern Lipe sheep carried out in the present study with the morphometric parameters of the native form of this breed described in 1935. The comparative analysis revealed the development dynamics of the local Lipe sheep in the traditional habitat, over a period of nearly one century. Throughout this period, different factors affecting Lipe sheep management, such as biogeografic, agro-economic, sociocultural and others, led to significant population erosion, and to the current status of the Lipe sheep as an endangered genetic resource. Although a slight increase has been registered in some body measurements, the major body indexes of the Lipe sheep, such as body format, body compactness, body massiveness, body proportion, pelvic, and head index, remained without significant fluctuations over the last century (p>0.05). Body length of the modern type still exceeds the height at withers, which was also recorded in the native Lipe sheep nearly 100 years ago. A prominent sexual dimorphism is still evident. Therefore, our comparative analysis showed no significant differences between the native and modern form of Lipe sheep in their body format and growth potential. The slight increases in absolute body measurements of the modern Lipe type we recorded could be linked to better housing conditions and improved quality of feed, without application of strategic selection measures over the last 100 years. The importance of conservation of this ovine resource is less economical, but mostly cultural, historical and heritage oriented, and still essential for the survival of the breed.

Mots clés

  • autochthonous genetic resource
  • conservation
  • phenotype characterization
  • Lipe type Zackel sheep
access type Accès libre

Effects of Breed/Species and Gender on Platelet Concentration in Autologous Platelet Rich Plasma

Publié en ligne: 01 Jan 2019
Pages: 474 - 483

Résumé

Abstract

Platelet rich plasma (PRP) is an autologous biological product harvested by consecutive centrifugations of whole blood and separation of plasma in a stepwise protocol. PRP has been successfully used to stimulate healing in orthopedic and dermatological conditions, both in humans and animals. The principle is the fact that α- granules inside platelets contain a high concentration of growth factors, that once released can interfere with cellular communication and speed up healing. Standardization of PRP requires establishing a gold standard for the preparation and evaluation of the product, especially considering that platelet concentration and, therefore, growth factor concentration, might vary due to a number of variables. Factors such as age, gender, race or breed, and immune status of the patient might interfere with PRP quality and with treatment results, although little is known about such interferences. This research investigated the effect of breed/species and gender in platelet concentration in autologous PRP from horses and mules. The results demonstrate that Quarter Horses provided PRP with the greatest amount of platelets, although mules had a higher concentration percentages in relation to the initial platelet counts.

Mots clés

  • biological products
  • blood platelets
  • horses
  • methods
access type Accès libre

Influence of High Frequency Electromagnetic Fields Produced by Antennas for Mobile Communication on the Structure of the Pancreas in Rats: Histological and Unbiased Stereological Analysis

Publié en ligne: 01 Jan 2019
Pages: 484 - 501

Résumé

Abstract

The emission of high frequency electromagnetic fields (HF EMF) produced by antennas for mobile communications has been controversially alleged to have adverse health effects. The aim of our work was to examine whether there are effects on living organisms from HF EMF produced by mobile communication antennas. In this experiment Wistar strain rats were exposed to HF EMF with the following characteristics: 1.9 GHz frequency, 0.24 A/m intensity, electric field strength of 4.79 V/m, and SAR (specific absorption rate) value of 2.0 W/m2. Exposure time was 7 hours per day, 5 days per week, over the course of sixty days. This experiment was conducted on a total of 30 male rats divided randomly into two equal groups: one group of animals was exposed to GSM fields (Global System of antennas for Mobile Communications) as described above whereas the other group of animals was not exposed to any GSM fields. In our study, results show that the quantity, diameter and numerical density of the islets of Langerhans in the pancreatic tissue increased in rats exposed to HF EMF compared to the unexposed group. The volume density, number and numerical density of pancreatic cells also changed in rats that were exposed to the HF EMF compared to the unexposed group. Our study shows a change in the stereological and histological parameters of rat pancreatic tissue due to the effects of HF EM fields produced by antennas for mobile communication.

Mots clés

  • HF EMF
  • islets of Langerhans
  • connective cells
  • endothelial cells
  • exocrine cells
access type Accès libre

Effect of Flax-Seed Enriched Concentrate Supplementation in Grazing Pramenka Breed Lamb’s Diet on Omental Fat Fatty Acids

Publié en ligne: 01 Jan 2019
Pages: 502 - 511

Résumé

Abstract

This research is aimed at determining the impact of supplementing extensively reared lambs with a limited amount of flax seed enriched-concentrate on the fatty acid profile of the lamb meat (omental fat depot). A total of 96 60-day old Pramenka breed lambs (23.85±3.98 kg live body weight; 60±10 days of age; 42 males and 54 females) raised on pasture with their dams were randomly divided in three experimental groups: control (CON) or extensive rearing group, which did not receive any supplementary feed; concentrate (CC) group, receiving 300 g of a conventional commercial concentrate feed per animal daily, and concentrate-flax seed (CC-FS) group, receiving 300 g/day of the concentrate supplemented with 5% of flax seed. After 60 days of experimental period, 10 lambs were selected (5 males and 5 females) from each group and slaughtered. Omental fat samples were taken and analyzed to determine their fatty acid profile. Significant (P>0.05) differences in the fatty acid profile between CON and CC groups were small. However, enrichment of the concentrate with 5% flax seed increased the percentages of PUFA and n-3 PUFA when compared to both CON and CC groups and thus slightly improved the nutritional characteristics of the omental fat. The effect of sex on the fatty acid profile was only observed for C20:4 n-6 and C20:5 n-3 (P < 0.05).

Mots clés

  • flax seed
  • fat depot
  • fatty acid
  • sheep nutrition
10 Articles
access type Accès libre

Production of Traditional Meat Products in Small and Micro Establishments in Serbia: Current Status and Future Perspectives

Publié en ligne: 01 Jan 2019
Pages: 373 - 390

Résumé

Abstract

The production of traditional meat products has a considerable potential in the Republic of Serbia, particularly in small and micro (household) establishments. Among a large number of traditional meat products, dry fermented sausages and dried meat products are the most important and commonly appreciated by consumers. There is, however, a need for a better standardization of the production in this meat sector, and also implementation of necessary food hygiene rules and HACCP principles according to hygiene regulations. There are provisions in the Food Safety Law, stating the principles of flexibility, which would allow for traditional meat producers and their associations to apply for derogations in food hygiene regulations. This would enable traditional small and micro food business operators to better use their resources, relax administrative burden and use traditional production methods in the most appropriate manner, always respecting hygiene and safety principals. This review paper analyses the current status of traditional meat production in small and micro establishments in Serbia, emphasizing a need for further improvements in food safety management and standardization.

Mots clés

  • food
  • traditional
  • hygiene
  • safety
  • management
  • flexibility
access type Accès libre

Mycobacteriosis in Farmed Sea Bream (Sparus aurata) Caused By Mycobacterium frederiksbergense in Turkey

Publié en ligne: 01 Jan 2019
Pages: 391 - 400

Résumé

Abstract

Over the past decades, different mycobacteriosis agents have become important fish pathogens. The present study describes a different mycobacteriosis in farmed sea bream (Sparus aurata) caused by Mycobacterium frederiksbergense in Turkey. Affected 15 fish, weighing 15 to 20 g, showed lethargy, stunted growth, pale skin, dorsal fin necrosis, and a significant level of mortality (40 %) in fish stocks. Internally, no multifocal white-colored granulomas in the visceral organs were observed. Inoculation of the visceral organs onto Löwenstein-Jensen medium and Tryptic Soy Agar (1.5% NaCl) slants produced only fast-growing (2-3 weeks), orange to yellow-colored, photochromogenic acid-fast colonies. Ziehl-Nielsen positive bacterial isolates were identified using a commercially available line probe assay (Genotype Mycobacterium CM/AS assay) and with 16S rRNA gene sequencing analysis based on 16S rRNA gene sequencing, fifteen isolates were identified as Mycobacterium frederiksbergense. Histopathologically, epithelioid cell granulomas were not observed in any vicseral organs but acid-fast bacteria were detected in the liver, kidney, spleen, and heart tissue. This study shows that asystemic mycobacteriosis is observed in sea bream with high mortality.

Mots clés

  • sea bream
  • Genotype Mycobacterium CM/AS assay
  • 16S rRNA gene
access type Accès libre

Application of the 3R Concept in the Production of European Antiviperinum on Horses – Multisite, Low Volumes Immunization Protocol and Elisa

Publié en ligne: 01 Jan 2019
Pages: 401 - 419

Résumé

Abstract

During time, both professionals and general public became aware of the importance of animal welfare. This term not only covers endangered wild animal species, animals used in food industry, pets and experimental animals, but also animals used in production of biologics. The implementation of the 3R concept (Replacement, Reduction and Refinement) is especially important in this type of production. In this article, we describe for the first time the low dose, low volume and multi-site immunization protocol, as well as appropriate ELISA we developed for production of European anti-viper (V. ammodytes, long horned) antivenom in horses, which can help to significantly improve the welfare of the used animals.

Mots clés

  • adjuvant
  • animal welfare
  • antivenom production
  • European vipers
  • horses
  • immunization schedule
access type Accès libre

Relationships Between Concentrations of Biological Variables in Eye Fluids and Blood After Exercise in Lidia Cattle

Publié en ligne: 01 Jan 2019
Pages: 420 - 433

Résumé

Abstract

Lidia cattle are a heterogeneous Iberian cattle population known for its natural aggressiveness and resistance to traditional handling procedures making in vivo blood sampling and biological fluid collections extremely difficult. Blood variables are influenced by physical exertion and stressful situations; consequently, post-mortem blood analysis does not reflect basal concentrations for this species. Nevertheless, ocular fluids (aqueous and vitreous humour) maintain their stable composition after death and maybe could be used to estimate ante-mortem blood concentrations. So, 15 bulls which had fought (for 15-20 minutes) and, subsequently after death, blood, aqueous and vitreous humour were sampled. Total protein, albumin, triglycerides, uric acid, urea, AST, ALT, GGT, AP, CK, LDH, cholesterol, creatinine, glucose and lactate were measured. Statistical analysis and correlation coefficients between the three fluids were carried out. All variables showed high plasma concentrations of glucose, uric acid, LDH and CK, compared with normal bovine concentrations. Apart from urea, all plasma concentrations were greater than those found in ocular fluids. The measured enzymes activities were higher in the vitreous than in the aqueous humour, but only marked differences in uric acid, lactate, AP and AST were found. There was a significant correlation between creatinine in the plasma and aqueous humour, and between albumin and GGT in the plasma and vitreous humour. Glucose, creatinine and urea exhibit a high correlation between ocular fluids. All plasma concentrations were clearly modified, however ocular fluids do not seem affected, thus establishing important correlations between the blood and intraocular fluids.

Mots clés

  • aqueous humor
  • vitreous humor
  • biochemical variables
  • intraocular fluids
  • blood
  • metabolism
  • Lidia cattle
access type Accès libre

Histopathological Characteristics and Expression of CDV-NP Antigen in the Brain of Serologically Positive Spontaneously Infected Red Foxes (Vulpes Vulpes) In Western Serbia

Publié en ligne: 01 Jan 2019
Pages: 434 - 444

Résumé

Abstract

Canine distemper virus (CDV) is a worldwide distributed RNA virus that can cause severe disease in carnivore and non-carnivore species. Red foxes are highly susceptible and may act as a reservoir of the virus. As in other wild species, distemper in red foxes can manifest as acute, systemic and chronic nervous form. In the present study, we detected antibodies against CDV among red foxes in Western Serbia, and analyzed histopathologically and immunohistochemically for CDV nuclear protein antigen (CDV-NP) brain samples derived from seropositive animals. Seroprevalence of CDV antibodies was 36.8%. Histopathological changes included gliosis, neuronal degeneration, satellitosis, mononuclear inflammation, demyelination and presence of inclusion bodies. Immunostaining showed a diffuse presence of CDV-NP antigen, mainly in the cytoplasm of astrocytes and neurons. Results of this work contribute to the opinion that red foxes act as a potential reservoir of CDV and underline the importance of routine vaccination of dogs that could come in close contact with these animals. Potential active surveillance program would give a better insight in the degree of CDV infection in wildlife.

Mots clés

  • brain
  • canine distemper virus
  • histopathology
  • immunohistochemistry
  • red fox
access type Accès libre

Antihelminic Activity of Carvacrol, Thymol, Cinnamaldehyde and P-Cymen Against the Free-Living Nematode Caenorhabditis elegans and Rat Pinworm Syphacia muris

Publié en ligne: 01 Jan 2019
Pages: 445 - 456

Résumé

Abstract

In the present study we tested the dose andh time dependence of the antinematodal effects of carvacrol and tyhmol on Caenorabditis elegans, and the efficacy of carvacrol, thymol, p-cymene and cinnamaldehyde,which were administrated in the drinking water of rats naturally infected with the pinworm Syphacia muris. The control treatment of the infected rats was carried out with piperazine. Thymol caused a dose and time-dependent mortality in adult C. elegans. The value of the Median Lethal Concentration (LC50) of thymol was 117.9nM after 24h and 62.89 nM after 48h of exposure. Carvacrol exhibited a higher antinematodal efficiency than thymol. The LC50 of carvacrol, after 24 hours of exposure, was 53.03 nM, while after 48 hours it was 33.83 nM. On the other hand, piperazine showed an extremely high efficacy against S. muris infection in rats. Piperazine, at a dose of 625 mg/kg bw, administered in drinking water continuously for 10 days, eliminates the infection completely. However, none of the investigated active ingredients of essential oils were effective against S. muris. The reason for the lack of efficiency may be due to their pharmacokinetic properties. A relatively low amount of, orally administered, active ingredients of essential oils reaches the distal segments of the gastrointestinal tract, where S. muris inhabits the gut (colon and cecum). The obtained results, on C. elegans, indicate a clear dose and time-dependent antinematodal effect of thymol and carvacrol. However, for clinical application, it is necessary to examine the efficacy of microencapsulated formulations with a controlled release of active ingredients of essential oils in certain parts of the gastrointestinal tract.

Mots clés

  • carvacrol
  • thymol
  • piperazine
access type Accès libre

In Situ Programme for the Conservation of the Autochthonous Lipe Type of Zackel Sheep

Publié en ligne: 01 Jan 2019
Pages: 457 - 473

Résumé

Abstract

The aim of this study was to emphasize the importance of conservation of the Lipe sheep, as a local endangered type of Zackel breed, and to perform a comprehensive phenotypic characterization in order to ensure a better use and preservation of this genetic resource. In addition, we compared the results of a detailed morphometric characterization of the modern Lipe sheep carried out in the present study with the morphometric parameters of the native form of this breed described in 1935. The comparative analysis revealed the development dynamics of the local Lipe sheep in the traditional habitat, over a period of nearly one century. Throughout this period, different factors affecting Lipe sheep management, such as biogeografic, agro-economic, sociocultural and others, led to significant population erosion, and to the current status of the Lipe sheep as an endangered genetic resource. Although a slight increase has been registered in some body measurements, the major body indexes of the Lipe sheep, such as body format, body compactness, body massiveness, body proportion, pelvic, and head index, remained without significant fluctuations over the last century (p>0.05). Body length of the modern type still exceeds the height at withers, which was also recorded in the native Lipe sheep nearly 100 years ago. A prominent sexual dimorphism is still evident. Therefore, our comparative analysis showed no significant differences between the native and modern form of Lipe sheep in their body format and growth potential. The slight increases in absolute body measurements of the modern Lipe type we recorded could be linked to better housing conditions and improved quality of feed, without application of strategic selection measures over the last 100 years. The importance of conservation of this ovine resource is less economical, but mostly cultural, historical and heritage oriented, and still essential for the survival of the breed.

Mots clés

  • autochthonous genetic resource
  • conservation
  • phenotype characterization
  • Lipe type Zackel sheep
access type Accès libre

Effects of Breed/Species and Gender on Platelet Concentration in Autologous Platelet Rich Plasma

Publié en ligne: 01 Jan 2019
Pages: 474 - 483

Résumé

Abstract

Platelet rich plasma (PRP) is an autologous biological product harvested by consecutive centrifugations of whole blood and separation of plasma in a stepwise protocol. PRP has been successfully used to stimulate healing in orthopedic and dermatological conditions, both in humans and animals. The principle is the fact that α- granules inside platelets contain a high concentration of growth factors, that once released can interfere with cellular communication and speed up healing. Standardization of PRP requires establishing a gold standard for the preparation and evaluation of the product, especially considering that platelet concentration and, therefore, growth factor concentration, might vary due to a number of variables. Factors such as age, gender, race or breed, and immune status of the patient might interfere with PRP quality and with treatment results, although little is known about such interferences. This research investigated the effect of breed/species and gender in platelet concentration in autologous PRP from horses and mules. The results demonstrate that Quarter Horses provided PRP with the greatest amount of platelets, although mules had a higher concentration percentages in relation to the initial platelet counts.

Mots clés

  • biological products
  • blood platelets
  • horses
  • methods
access type Accès libre

Influence of High Frequency Electromagnetic Fields Produced by Antennas for Mobile Communication on the Structure of the Pancreas in Rats: Histological and Unbiased Stereological Analysis

Publié en ligne: 01 Jan 2019
Pages: 484 - 501

Résumé

Abstract

The emission of high frequency electromagnetic fields (HF EMF) produced by antennas for mobile communications has been controversially alleged to have adverse health effects. The aim of our work was to examine whether there are effects on living organisms from HF EMF produced by mobile communication antennas. In this experiment Wistar strain rats were exposed to HF EMF with the following characteristics: 1.9 GHz frequency, 0.24 A/m intensity, electric field strength of 4.79 V/m, and SAR (specific absorption rate) value of 2.0 W/m2. Exposure time was 7 hours per day, 5 days per week, over the course of sixty days. This experiment was conducted on a total of 30 male rats divided randomly into two equal groups: one group of animals was exposed to GSM fields (Global System of antennas for Mobile Communications) as described above whereas the other group of animals was not exposed to any GSM fields. In our study, results show that the quantity, diameter and numerical density of the islets of Langerhans in the pancreatic tissue increased in rats exposed to HF EMF compared to the unexposed group. The volume density, number and numerical density of pancreatic cells also changed in rats that were exposed to the HF EMF compared to the unexposed group. Our study shows a change in the stereological and histological parameters of rat pancreatic tissue due to the effects of HF EM fields produced by antennas for mobile communication.

Mots clés

  • HF EMF
  • islets of Langerhans
  • connective cells
  • endothelial cells
  • exocrine cells
access type Accès libre

Effect of Flax-Seed Enriched Concentrate Supplementation in Grazing Pramenka Breed Lamb’s Diet on Omental Fat Fatty Acids

Publié en ligne: 01 Jan 2019
Pages: 502 - 511

Résumé

Abstract

This research is aimed at determining the impact of supplementing extensively reared lambs with a limited amount of flax seed enriched-concentrate on the fatty acid profile of the lamb meat (omental fat depot). A total of 96 60-day old Pramenka breed lambs (23.85±3.98 kg live body weight; 60±10 days of age; 42 males and 54 females) raised on pasture with their dams were randomly divided in three experimental groups: control (CON) or extensive rearing group, which did not receive any supplementary feed; concentrate (CC) group, receiving 300 g of a conventional commercial concentrate feed per animal daily, and concentrate-flax seed (CC-FS) group, receiving 300 g/day of the concentrate supplemented with 5% of flax seed. After 60 days of experimental period, 10 lambs were selected (5 males and 5 females) from each group and slaughtered. Omental fat samples were taken and analyzed to determine their fatty acid profile. Significant (P>0.05) differences in the fatty acid profile between CON and CC groups were small. However, enrichment of the concentrate with 5% flax seed increased the percentages of PUFA and n-3 PUFA when compared to both CON and CC groups and thus slightly improved the nutritional characteristics of the omental fat. The effect of sex on the fatty acid profile was only observed for C20:4 n-6 and C20:5 n-3 (P < 0.05).

Mots clés

  • flax seed
  • fat depot
  • fatty acid
  • sheep nutrition

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