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Fig. 1
Promoter region of MEIS1 with marked TSS
Fig. 2
CpG island prediction generated by MethPrimer software
Fig. 3
PCR optimization. Figure 3A shows a positive signal generated by methylated probes using methylated control DNA (column B01); Figure 3B shows a positive signal generated by unmethylated probes using unmethylated control DNA (column B04). The positive signal generated by unmethylated probes in line B02 demonstrates the cross-reactivity of unmethylated probes with methylated control DNA.
Fig. 4
Concentration gradient: A) methylated DNA control using the methylated probe. B) unmethylated DNA control using the unmethylated probe.