<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec><h3>Background</h3>
<p>The ambiguity of renal cell carcinoma (RCC) symptoms hinders early diagnosis, thereby contributing to high mortality rates. By attaching to the 3′-untranslated region (UTR) of the target gene, microRNAs (miRNAs) exert significant control over the expression of genes.</p>
</sec>
<sec><h3>Objectives</h3>
<p>To investigate the influence of miR-30c-2-3p and DNA topoisomerase II alpha (TOP2A) on RCC growth and the mechanisms underlying the regulation of its expression.</p>
</sec>
<sec><h3>Methods</h3>
<p>The expression of miRNA-30c-2-3p and <italic>TOP2A</italic> in RCC cells was examined using quantitative real-time polymerase chain reaction (qRT-PCR). MiR-30c-2-3p mimics, its inhibitors, and controls, as well as TOP2A short hairpin RNA (shRNA) and controls, were used to transfect the human RCC cell lines 786-O, Caki-1, and ACHN. Additionally, the roles of miRNA-30c-2-3p and TOP2A in the growth of RCC were evaluated using the cell counting kit (CCK)-8 test, colony formation assay, apoptosis analysis, and Western blotting. Meanwhile, binding of miRNA-30c-2-3p and TOP2A was verified using dual-luciferase reporter assays and Western blotting.</p>
</sec>
<sec><h3>Results</h3>
<p>miR-30c-2-p is underexpressed in RCC cells. Overexpression of miR-30c-2-p promotes apoptosis and inhibits proliferation of ACHN, Caki-1, and 786-O cells. miR-30c-2-3p targets TOP2A, which is elevated in RCC tissues and cells, whereas TOP2A silencing inhibits the proliferation ability of RCC cells. The miRNA-30c-2-3p inhibitor compromises TOP2A shRNA-induced apoptosis of RCC. RCC cells cotransfected with miRNA-30c-2-3p inhibitors and TOP2A shRNAs have a higher proliferation rate than those transfected with only TOP2A shRNAs.</p>
</sec>
<sec><h3>Conclusions</h3>
<p>Collectively, our results verify that miRNA-30c-2-3p has a tumor suppressor property. miRNA-30c-2-3p inhibits the proliferation of RCC through regulation of TOP2A. The data provide a viable therapeutic target for RCC.</p>
</sec>
</abstract>

Background
The ambiguity of renal cell carcinoma (RCC) symptoms hinders early diagnosis, thereby contributing to high mortality rates. By attaching to the 3′-untranslated region (UTR) of the target gene, microRNAs (miRNAs) exert significant control over the expression of genes.

Objectives
To investigate the influence of miR-30c-2-3p and DNA topoisomerase II alpha (TOP2A) on RCC growth and the mechanisms underlying the regulation of its expression.

Methods
The expression of miRNA-30c-2-3p and TOP2A in RCC cells was examined using quantitative real-time polymerase chain reaction (qRT-PCR). MiR-30c-2-3p mimics, its inhibitors, and controls, as well as TOP2A short hairpin RNA (shRNA) and controls, were used to transfect the human RCC cell lines 786-O, Caki-1, and ACHN. Additionally, the roles of miRNA-30c-2-3p and TOP2A in the growth of RCC were evaluated using the cell counting kit (CCK)-8 test, colony formation assay, apoptosis analysis, and Western blotting. Meanwhile, binding of miRNA-30c-2-3p and TOP2A was verified using dual-luciferase reporter assays and Western blotting.

Results
miR-30c-2-p is underexpressed in RCC cells. Overexpression of miR-30c-2-p promotes apoptosis and inhibits proliferation of ACHN, Caki-1, and 786-O cells. miR-30c-2-3p targets TOP2A, which is elevated in RCC tissues and cells, whereas TOP2A silencing inhibits the proliferation ability of RCC cells. The miRNA-30c-2-3p inhibitor compromises TOP2A shRNA-induced apoptosis of RCC. RCC cells cotransfected with miRNA-30c-2-3p inhibitors and TOP2A shRNAs have a higher proliferation rate than those transfected with only TOP2A shRNAs.

Conclusions
Collectively, our results verify that miRNA-30c-2-3p has a tumor suppressor property. miRNA-30c-2-3p inhibits the proliferation of RCC through regulation of TOP2A. The data provide a viable therapeutic target for RCC.

miR-30c-2-3p suppresses the proliferation of human renal cell carcinoma cells by targeting TOP2A

Clinical Laboratory of Affiliated Hospital of Yan’an University

Asian Biomedicine

This work is licensed under the Creative Commons Attribution 4.0 International License.

Background
The ambiguity of renal cell carcinoma (RCC) symptoms hinders early diagnosis, thereby contributing to high mortality rates. By attaching to the...

miR-30c-2-3p suppresses the proliferation of human renal cell carcinoma cells by targeting TOP2A

Article Category: Original article

Publié en ligne: 09 oct. 2023

Pages: 124 - 135

DOI: https://doi.org/10.2478/abm-2023-0052

Mots clés
apoptosis, miR-30c-2-3p, renal cell carcinoma, TOP2A, treatment

© 2023 Xiaoyong Huang et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

miR-30c-2-3p suppresses the proliferation of human renal cell carcinoma cells by targeting TOP2A

Article Category: Original article

Publié en ligne: 09 oct. 2023

Pages: 124 - 135

DOI: https://doi.org/10.2478/abm-2023-0052

Mots clésapoptosis, miR-30c-2-3p, renal cell carcinoma, TOP2A, treatment

© 2023 Xiaoyong Huang et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Mots clés
apoptosis, miR-30c-2-3p, renal cell carcinoma, TOP2A, treatment