<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">We investigated the muscle tissue of a teleost <italic>Cyprinus carpio</italic> L. to find out whether <italic>N</italic>-acetylcysteine (NAC), <italic>alpha</italic>-lipoic acid (LA), taurine (TAU), and curcumin (CUR) were able to counteract oxidative stress induced by acute exposure to cadmium (Cd). The muscle tissue was dissected 96 h after a single intraperitoneal injection of Cd (5 mg kg-1) and of antioxidant substances (50 mg kg-1). Using spectrophotometry, we determined the glutathione redox status, lipid peroxidation levels and the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione disulphide reductase (GR). Accumulation of Cd in the muscle was analysed using inductively coupled plasma - optical emission spectrometry (ICP-OES).All substances lowered Cd levels in the following order of efficiency; LA=NAC>TAU=CUR. Cadmium increased SOD activity, but CAT activity declined, regardless of antioxidant treatment. Treatment with CUR induced GPx activity. Treatment with TAU lowered Cd due to higher total glutathione (tGSH). The most effective substances on lipid peroxidation were LA and NAC due to a greater Cd-lowering potential. It seems that the protective role of TAU, LA, and NAC is not necessarily associated with antioxidant enzymes, but rather with their own activity.</abstract>

We investigated the muscle tissue of a teleost Cyprinus carpio L. to find out whether N-acetylcysteine (NAC), alpha-lipoic acid (LA), taurine (TAU), and curcumin (CUR) were able to counteract oxidative stress induced by acute exposure to cadmium (Cd). The muscle tissue was dissected 96 h after a single intraperitoneal injection of Cd (5 mg kg-1) and of antioxidant substances (50 mg kg-1). Using spectrophotometry, we determined the glutathione redox status, lipid peroxidation levels and the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione disulphide reductase (GR). Accumulation of Cd in the muscle was analysed using inductively coupled plasma - optical emission spectrometry (ICP-OES).All substances lowered Cd levels in the following order of efficiency; LA=NAC>TAU=CUR. Cadmium increased SOD activity, but CAT activity declined, regardless of antioxidant treatment. Treatment with CUR induced GPx activity. Treatment with TAU lowered Cd due to higher total glutathione (tGSH). The most effective substances on lipid peroxidation were LA and NAC due to a greater Cd-lowering potential. It seems that the protective role of TAU, LA, and NAC is not necessarily associated with antioxidant enzymes, but rather with their own activity.

Antioxidative Effects of <italic>N</italic>-acetylcysteine, Lipoic Acid, Taurine, and Curcumin in the Muscle of <italic>Cyprinus carpio</italic> L. Exposed to Cadmium

Faculty of Science and Letters, Department of Biology, Adiyaman University, Adiyaman, Turkey

Faculty of Aquaculture, University of Mersin, Yenişehir Campus, Mersin, Turkey

Archives of Industrial Hygiene and Toxicology

Antioxidative Effects of -acetylcysteine, Lipoic Acid, Taurine, and Curcumin in the Muscle of  L. Exposed to Cadmium

We investigated the muscle tissue of a teleost Cyprinus carpio L. to find out whether N-acetylcysteine (NAC), alpha-lipoic acid (LA), taurine (TAU), and...

Antioxidative Effects of N-acetylcysteine, Lipoic Acid, Taurine, and Curcumin in the Muscle of Cyprinus carpio L. Exposed to Cadmium

Publié en ligne: 21 mars 2011

Pages: 1 - 9

DOI: https://doi.org/10.2478/10004-1254-62-2011-2082

Mots clés
akumulacija, antioksidansi, mišićno tkivo, oksidativni stres, riba

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