Emodin Reduces the Activity of (1,3)-β -D-glucan Synthase from Candida albicans and Does Not Interact with Caspofungin
| 10 déc. 2018
À propos de cet article
Article Category: original-paper
Publié en ligne: 10 déc. 2018
Pages: 463 - 470
Reçu: 20 juin 2018
Accepté: 04 sept. 2018
DOI: https://doi.org/10.21307/pjm-2018-054
Mots clés
© 2018 Monika Janeczko published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Fig. 1.
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Fig. 3.
In vitro activity of emodin and caspofungin alone and in combination assessed by the broth dilution method against clinical strains of C. albicans isolated from vaginas.
| Strain No. | MIC of the drug used alone (µg/ml) | MIC of the drug used in combination (µg/ml) | FICI | Interaction | |
|---|---|---|---|---|---|
| Emodin | Caspofungin | Emodin + Caspofungin | |||
| 1 | 50 | 0.3 | 50 + 0.3 | 2 | Indifferent |
| 2 | 50 | 0.6 | 50 + 0.6 | 2 | Indifferent |
| 3 | 25 | 0.15 | 25 + 0.3 | 3 | Indifferent |
| 4 | 50 | 0.3 | 50 + 0.3 | 2 | Indifferent |
| 5 | 25 | 0.15 | 25 + 0.3 | 3 | Indifferent |
| 6 | 50 | 0.07 | 25 + 0.15 | 2.64 | Indifferent |
| 7 | 50 | 0.3 | 50 + 0.6 | 3 | Indifferent |
| 8 | 50 | 0.6 | 25 + 0.3 | 1 | Indifferent |
| 9 | 12.5 | 0.3 | 25 + 0.3 | 3 | Indifferent |
| 10 | 50 | 0.15 | 50 + 0.6 | 5 | Antagonism |
| 11 | 50 | 0.07 | 50 + 0.15 | 3 | Indifferent |
| 12 | 50 | 0.6 | 50 + 0.3 | 1.5 | Indifferent |
| 13 | 25 | 0.3 | 50 + 0.3 | 3 | Indifferent |
| 14 | 25 | 0.6 | 50 + 0.15 | 2.25 | Indifferent |
| 15 | 25 | 0.3 | 50 + 0.15 | 2.5 | Indifferent |
| 16 | 12.5 | 0.03 | 25 + 0.15 | 5 | Antagonism |
| 17 | 12.5 | 0.6 | 12.5 + 0.6 | 2 | Indifferent |
| 18 | 50 | 0.3 | 50 + 0.6 | 3 | Indifferent |
| 19 | 50 | 0.15 | 25 + 0.07 | 1 | Indifferent |
| 20 | 50 | 0.6 | 50 + 0.3 | 1.5 | Indifferent |
In vitro activity of emodin and caspofungin alone and in combination assessed by the broth dilution assay against clinical strains of C. albicans isolated from urinary tracts.
| Strain No. | MIC of the drug used alone (µg/ml) | MIC of the drug used in combination (µg/ml) | FICI | Interaction | |
|---|---|---|---|---|---|
| Emodin | Caspofungin | Emodin + Caspofungin | |||
| 1 | 12.5 | 0.15 | 12.5 + 0.3 | 3 | Indifferent |
| 2 | 12.5 | 0.07 | 12.5 + 0.3 | 5.28 | Antagonism |
| 3 | 25 | 0.3 | 25 + 0.6 | 3 | Indifferent |
| 4 | 25 | 0.3 | 25 + 0.3 | 2 | Indifferent |
| 5 | 50 | 0.6 | 50 + 1.2 | 3 | Indifferent |
| 6 | 50 | 0.15 | 50 + 0.3 | 3 | Indifferent |
| 7 | 12.5 | 0.15 | 12.5 + 0.3 | 3 | Indifferent |
| 8 | 12.5 | 0.07 | 12.5 + 0.3 | 5.28 | Antagonism |
| 9 | 25 | 0.03 | 25 + 0.15 | 6 | Antagonism |
| 10 | 25 | 0.3 | 25 + 0.15 | 1.5 | Indifferent |
| 11 | 25 | 0.15 | 25 + 0.3 | 3 | Indifferent |
| 12 | 50 | 0.6 | 50 + 0.3 | 1.5 | Indifferent |
| 13 | 50 | 0.3 | 50 + 0.3 | 2 | Indifferent |
| 14 | 50 | 0.6 | 50 + 0.6 | 2 | Indifferent |
| 15 | 25 | 0.3 | 25 + 0.15 | 1.5 | Indifferent |
| 16 | 6.25 | 0.03 | 12.5 + 0.06 | 4 | Indifferent |
| 17 | 50 | 0.6 | 50 + 0.6 | 2 | Indifferent |
| 18 | 50 | 0.3 | 50 + 0.3 | 2 | Indifferent |
| 19 | 50 | 0.15 | 25 + 0.15 | 1.5 | Indifferent |
| 20 | 25 | 0.6 | 50 + 0.6 | 3 | Indifferent |
Effect of sorbitol on the MICs of emodin and antibiotics against C. albicans ATCC 10231.
| MIC (µg/ml) | ||
|---|---|---|
| Without sorbitol | With sorbitol | |
| Emodin | 12.5 | 25 |
| Caspofungin | 0.15 | 0.6 |
| Amphotericin B | 1 | 1 |