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The Effect of Silver Nanoparticles on Listeria monocytogenes PCM2191 Peptidoglycan Metabolism and Cell Permeability

KATARZYNA MARKOWSKA
KATARZYNA MARKOWSKA
, 
ANNA MARIA GRUDNIAK
ANNA MARIA GRUDNIAK
, 
BARBARA MILCZAREK
BARBARA MILCZAREK
 et 
KRYSTYNA IZABELLA WOLSKA
KRYSTYNA IZABELLA WOLSKA
   | 04 sept. 2018
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Article Category: original-paper
Publié en ligne: 04 sept. 2018
Pages: 315 - 320
Reçu: 11 janv. 2018
Accepté: 10 mai 2018
DOI: https://doi.org/10.21307/pjm-2018-037
Mots clés
© 2018 Katarzyna Markowska et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Effect of AgNPs on L. monocytogenes growth (A) and survival (B).Solid lines – control; dashed lines – 0.25 MIC AgNPs; dotted lines – 0.5 MIC AgNPs. Mean ± SD values of triplicate cultures were shown.
Effect of AgNPs on L. monocytogenes growth (A) and survival (B).Solid lines – control; dashed lines – 0.25 MIC AgNPs; dotted lines – 0.5 MIC AgNPs. Mean ± SD values of triplicate cultures were shown.

Fig. 2.

Influence of AgNPs on lysozyme- or Triton X-100-induced autolysis/lysis of L. monocytogenes.White bar – control, buffer only; dark grey bar – lysozyme or Triton X-100; light grey bar – lysozyme or Triton X-100 + 0.25 MIC AgNPs; black bar – lysozyme or Triton X-100 + 0.5 MIC AgNPs. The results are mean of three independent experiments with every measurement done in triplicate ± SD. Statistically relevant differences (p < 0.05) were marked with asterisks.
Influence of AgNPs on lysozyme- or Triton X-100-induced autolysis/lysis of L. monocytogenes.White bar – control, buffer only; dark grey bar – lysozyme or Triton X-100; light grey bar – lysozyme or Triton X-100 + 0.25 MIC AgNPs; black bar – lysozyme or Triton X-100 + 0.5 MIC AgNPs. The results are mean of three independent experiments with every measurement done in triplicate ± SD. Statistically relevant differences (p < 0.05) were marked with asterisks.

Fig. 3.

Autolysis of isolated L. monocytogenes peptidoglycan in the presence of AgNPs.Absorbance A600 at time 0 was considered as 100%. Solid line – control; dashed line – 0.25 MIC AgNPs; dotted line – 0.5 MIC AgNPs. The results are mean of three independent experiments with every measurement done in triplicate ± SD. Statistically relevant difference (p < 0.05) was marked with an asterisk.
Autolysis of isolated L. monocytogenes peptidoglycan in the presence of AgNPs.Absorbance A600 at time 0 was considered as 100%. Solid line – control; dashed line – 0.25 MIC AgNPs; dotted line – 0.5 MIC AgNPs. The results are mean of three independent experiments with every measurement done in triplicate ± SD. Statistically relevant difference (p < 0.05) was marked with an asterisk.

Fig. 4.

Effect of AgNPs influence on the efflux of DNA and proteins from L. monocytogenes.White bars – control, no AgNPs added; dark grey bars – 0.25 MIC AgNPs; light grey bars – 0.5 MIC AgNPs; black bars – 100 μg/ml lysozyme (positive control). The results are mean of three independent experiments with every measurement done in triplicate ± SD. Statistically relevant difference (p < 0.05) were marked with asterisks.
Effect of AgNPs influence on the efflux of DNA and proteins from L. monocytogenes.White bars – control, no AgNPs added; dark grey bars – 0.25 MIC AgNPs; light grey bars – 0.5 MIC AgNPs; black bars – 100 μg/ml lysozyme (positive control). The results are mean of three independent experiments with every measurement done in triplicate ± SD. Statistically relevant difference (p < 0.05) were marked with asterisks.

Increase in cF efflux from L. monocytogenes caused by AgNPs.

AgNPs concentration% efflux of cF at time (min)
010
0 (negative control)72 ± 279 ± 1
0.25 MIC73 ± 284 ± 2
0.5 MIC74 ± 293* ± 2
eISSN:
2544-4646
Langue:
Anglais
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Sujets de la revue:
Life Sciences, Microbiology and Virology
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