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Biohydrogen Production by Antarctic Psychrotolerant Klebsiella sp. ABZ11

ABDULLAHI MOHAMMED
ABDULLAHI MOHAMMED
, 
MOHD FIRDAUS ABDUL-WAHAB
MOHD FIRDAUS ABDUL-WAHAB
, 
MAZLAN HASHIM
MAZLAN HASHIM
, 
ABDUL HAFIDZ OMAR
ABDUL HAFIDZ OMAR
, 
MOHD NADZRI MD REBA
MOHD NADZRI MD REBA
, 
MOHD FARID MUHAMAD SAID
MOHD FARID MUHAMAD SAID
, 
KAMARUZAMAN SOEED
KAMARUZAMAN SOEED
, 
SITI AISYAH ALIAS
SITI AISYAH ALIAS
, 
JERZY SMYKLA
JERZY SMYKLA
, 
MUSTAPHA ABBA
MUSTAPHA ABBA
 et 
ZAHARAH IBRAHIM
ZAHARAH IBRAHIM
   | 04 sept. 2018
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Article Category: original-paper
Publié en ligne: 04 sept. 2018
Pages: 283 - 290
Reçu: 02 nov. 2017
Accepté: 20 févr. 2018
DOI: https://doi.org/10.21307/pjm-2018-033
© 2018 Abdullahi Mohammed et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

The biohydrogen productivity of the five isolates screened.
The biohydrogen productivity of the five isolates screened.

Fig. 2.

Mean dry cell weight (DCW) of Klebsiella sp. ABZ11 during growth at 30°C.
Mean dry cell weight (DCW) of Klebsiella sp. ABZ11 during growth at 30°C.

Fig. 3.

Scanning electron micrograph of Klebsiella sp. ABZ11. Bar represents 1 µm scale.
Scanning electron micrograph of Klebsiella sp. ABZ11. Bar represents 1 µm scale.

Fig. 4.

A. Growth of Klebsiella sp. ABZ11 on blood agar plate. No blood haemolysis was observed after 48 h incubation at 30°C. B. Capsule staining of the ABZ11. No capsule is visible around the cells suggesting the non-pathogenicity of this strain.
A. Growth of Klebsiella sp. ABZ11 on blood agar plate. No blood haemolysis was observed after 48 h incubation at 30°C. B. Capsule staining of the ABZ11. No capsule is visible around the cells suggesting the non-pathogenicity of this strain.

Fig. 5.

Phylogenetic tree constructed using the partial 16S rRNA gene sequences by neighbor-joining method (19 nucleotides, 1303 data sets, branch length of 0.033 and all gaps removed) showing the relationship between ABZ11 and the 17 most closely related reference species. The gene sequence of Salmonella typhimurium X80681 was used as the outgroup. Bootstrap values (expressed as percentages of 1050 replications) are shown at the branch points. Bar represents 0.002 substitutions per nucleotide position.
Phylogenetic tree constructed using the partial 16S rRNA gene sequences by neighbor-joining method (19 nucleotides, 1303 data sets, branch length of 0.033 and all gaps removed) showing the relationship between ABZ11 and the 17 most closely related reference species. The gene sequence of Salmonella typhimurium X80681 was used as the outgroup. Bootstrap values (expressed as percentages of 1050 replications) are shown at the branch points. Bar represents 0.002 substitutions per nucleotide position.

Fig. 6.

Mean growth temperature range and optimum temperature of Klebsiella sp. ABZ11.
Mean growth temperature range and optimum temperature of Klebsiella sp. ABZ11.

Fig. 7.

Oxygen uptake by ABZ11 at different time intervals.
Oxygen uptake by ABZ11 at different time intervals.

Fig. 8.

Average biohydrogen productivity and carbohydrate uptake after 48 h fermentation of glucose (A, B), fructose (C, D) and sucrose (E, F) by Klebsiella sp. ABZ11.
Average biohydrogen productivity and carbohydrate uptake after 48 h fermentation of glucose (A, B), fructose (C, D) and sucrose (E, F) by Klebsiella sp. ABZ11.

Fig. 9.

Biohydrogen production kinetic of Klebsiella sp. ABZ11 at 5 g/l glucose concentration.
Biohydrogen production kinetic of Klebsiella sp. ABZ11 at 5 g/l glucose concentration.

Biohydrogen production and kinetic parameters of substrate utilization by Klebsiella sp. ABZ11.

Carbohydrate concentrations (g/l)57.51012.5
Biohydrogen production at initial incubation time and at maximum production (mol/l)GlucoseInitial0.21 ± 0.000.21 ± 0.022.35 ± 0.030.77 ± 0.01
Maximum26.23 ± 2.1823.80 ± 3.29*38.55 ± 2.19*21.11 ± 0.14*
FructoseInitial0.21 ± 0.000.47 ± 0.050.65 ± 0.460.81 ± 0.09
Maximum25.47 ± 2.0222.77 ± 2.0119.97 ± 1.6016.97 ± 0.12
SucroseInitial0.21 ± 0.000.23 ± 0.040.23 ± 0.014.52 ± 0.36
Maximum28.24 ± 2.96*22.37 ± 2.1918.21 ± 0.7715.91 ± 1.49
Ketic analysisYP/S (mol/mg)23.5317.0024.0911.11
YX/S (g/mg)0.1700.4240.5320.205
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