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Biohydrogen Production by Antarctic Psychrotolerant Klebsiella sp. ABZ11

ABDULLAHI MOHAMMED

ABDULLAHI MOHAMMED

  • Ibrahim Badamasi Babangida UniversityLapai, Niger State, Nigeria
  • Faculty of Biosciences and Medical Engineering, Universiti Teknologi MalaysiaJohor, Malaysia
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MOHAMMED, ABDULLAHI
, 
MOHD FIRDAUS ABDUL-WAHAB

MOHD FIRDAUS ABDUL-WAHAB

Faculty of Biosciences and Medical Engineering, Universiti Teknologi MalaysiaJohor, Malaysia
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ABDUL-WAHAB, MOHD FIRDAUS
, 
MAZLAN HASHIM

MAZLAN HASHIM

Geoscience and Digital Earth Centre (INSTeG), Universiti Teknologi MalaysiaJohor, Malaysia
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HASHIM, MAZLAN
, 
ABDUL HAFIDZ OMAR

ABDUL HAFIDZ OMAR

Sports Innovation Technology Centre (SITC), Universiti Teknologi MalaysiaJohor, Malaysia
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OMAR, ABDUL HAFIDZ
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MOHD NADZRI MD REBA

MOHD NADZRI MD REBA

Geoscience and Digital Earth Centre (INSTeG), Universiti Teknologi MalaysiaJohor, Malaysia
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MD REBA, MOHD NADZRI
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MOHD FARID MUHAMAD SAID

MOHD FARID MUHAMAD SAID

Automotive Development Centre (ADC), Universiti Teknologi MalaysiaJohor, Malaysia
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MUHAMAD SAID, MOHD FARID
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KAMARUZAMAN SOEED

KAMARUZAMAN SOEED

Sports Innovation Technology Centre (SITC), Universiti Teknologi MalaysiaJohor, Malaysia
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SOEED, KAMARUZAMAN
, 
SITI AISYAH ALIAS

SITI AISYAH ALIAS

  • Institute of Ocean and Earth Sciences, University of MalayaKuala Lumpur, Malaysia
  • National Antarctic Research Centre, Institute of Graduate Studies, University of MalayaKuala Lumpur, Malaysia
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ALIAS, SITI AISYAH
, 
JERZY SMYKLA

JERZY SMYKLA

  • Department of Biology and Marine Biology, University of North Carolina WilmingtonWilmington, USA
  • Department of Biodiversity, Institute of Nature Conservation, Polish Academy of SciencesKrakow, Poland
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SMYKLA, JERZY
, 
MUSTAPHA ABBA

MUSTAPHA ABBA

Department of Microbiology, Bauchi State University GadauBauchi, Nigeria
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ABBA, MUSTAPHA
 und 
ZAHARAH IBRAHIM

ZAHARAH IBRAHIM

Faculty of Biosciences and Medical Engineering, Universiti Teknologi MalaysiaJohor, Malaysia
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IBRAHIM, ZAHARAH
  
04. Sept. 2018
Biohydrogen Production by Antarctic Psychrotolerant Klebsiella sp. ABZ11's Cover Image
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Artikel-Kategorie: original-paper
Online veröffentlicht: 04. Sept. 2018
Seitenbereich: 283 - 290
Eingereicht: 02. Nov. 2017
Akzeptiert: 20. Feb. 2018
DOI: https://doi.org/10.21307/pjm-2018-033
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© 2018 Abdullahi Mohammed et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

The biohydrogen productivity of the five isolates screened.
The biohydrogen productivity of the five isolates screened.

Fig. 2.

Mean dry cell weight (DCW) of Klebsiella sp. ABZ11 during growth at 30°C.
Mean dry cell weight (DCW) of Klebsiella sp. ABZ11 during growth at 30°C.

Fig. 3.

Scanning electron micrograph of Klebsiella sp. ABZ11. Bar represents 1 µm scale.
Scanning electron micrograph of Klebsiella sp. ABZ11. Bar represents 1 µm scale.

Fig. 4.

A. Growth of Klebsiella sp. ABZ11 on blood agar plate. No blood haemolysis was observed after 48 h incubation at 30°C. B. Capsule staining of the ABZ11. No capsule is visible around the cells suggesting the non-pathogenicity of this strain.
A. Growth of Klebsiella sp. ABZ11 on blood agar plate. No blood haemolysis was observed after 48 h incubation at 30°C. B. Capsule staining of the ABZ11. No capsule is visible around the cells suggesting the non-pathogenicity of this strain.

Fig. 5.

Phylogenetic tree constructed using the partial 16S rRNA gene sequences by neighbor-joining method (19 nucleotides, 1303 data sets, branch length of 0.033 and all gaps removed) showing the relationship between ABZ11 and the 17 most closely related reference species. The gene sequence of Salmonella typhimurium X80681 was used as the outgroup. Bootstrap values (expressed as percentages of 1050 replications) are shown at the branch points. Bar represents 0.002 substitutions per nucleotide position.
Phylogenetic tree constructed using the partial 16S rRNA gene sequences by neighbor-joining method (19 nucleotides, 1303 data sets, branch length of 0.033 and all gaps removed) showing the relationship between ABZ11 and the 17 most closely related reference species. The gene sequence of Salmonella typhimurium X80681 was used as the outgroup. Bootstrap values (expressed as percentages of 1050 replications) are shown at the branch points. Bar represents 0.002 substitutions per nucleotide position.

Fig. 6.

Mean growth temperature range and optimum temperature of Klebsiella sp. ABZ11.
Mean growth temperature range and optimum temperature of Klebsiella sp. ABZ11.

Fig. 7.

Oxygen uptake by ABZ11 at different time intervals.
Oxygen uptake by ABZ11 at different time intervals.

Fig. 8.

Average biohydrogen productivity and carbohydrate uptake after 48 h fermentation of glucose (A, B), fructose (C, D) and sucrose (E, F) by Klebsiella sp. ABZ11.
Average biohydrogen productivity and carbohydrate uptake after 48 h fermentation of glucose (A, B), fructose (C, D) and sucrose (E, F) by Klebsiella sp. ABZ11.

Fig. 9.

Biohydrogen production kinetic of Klebsiella sp. ABZ11 at 5 g/l glucose concentration.
Biohydrogen production kinetic of Klebsiella sp. ABZ11 at 5 g/l glucose concentration.

Biohydrogen production and kinetic parameters of substrate utilization by Klebsiella sp_ ABZ11_

Carbohydrate concentrations (g/l)57.51012.5
Biohydrogen production at initial incubation time and at maximum production (mol/l)GlucoseInitial0.21 ± 0.000.21 ± 0.022.35 ± 0.030.77 ± 0.01
Maximum26.23 ± 2.1823.80 ± 3.29*38.55 ± 2.19*21.11 ± 0.14*
FructoseInitial0.21 ± 0.000.47 ± 0.050.65 ± 0.460.81 ± 0.09
Maximum25.47 ± 2.0222.77 ± 2.0119.97 ± 1.6016.97 ± 0.12
SucroseInitial0.21 ± 0.000.23 ± 0.040.23 ± 0.014.52 ± 0.36
Maximum28.24 ± 2.96*22.37 ± 2.1918.21 ± 0.7715.91 ± 1.49
Ketic analysisYP/S (mol/mg)23.5317.0024.0911.11
YX/S (g/mg)0.1700.4240.5320.205
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