Samplings of Millipedes in Japan and Scarab Beetles in Hong Kong result in five new Species of Pristionchus (Nematoda: Diplogastridae)
, , , , et | 03 déc. 2018
À propos de cet article
Publié en ligne: 03 déc. 2018
Pages: 587 - 610
DOI: https://doi.org/10.21307/jofnem-2018-044
Mots clés
© 2018 Natsumi Kanzaki et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Figure 13
Figure 14
The table shows the number of molecular differences in the 1.6kb SSU rRNA sequence for every pairwise comparison in the pacificus species-complex.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
|
0 | 8 | 5 | 10 | 6 | 3 | 6 | 21 | 16 | 35 | 25 | 31 | 42 | 36 |
|
|
– | 0 | 6 | 12 | 8 | 5 | 8 | 23 | 16 | 34 | 27 | 33 | 43 | 38 |
|
|
– | – | 0 | 1 | 4 | 2 | 5 | 18 | 14 | 27 | 21 | 27 | 38 | 33 |
|
|
– | – | – | 0 | 10 | 1 | 4 | 18 | 14 | 32 | 21 | 27 | 38 | 33 |
|
|
– | – | – | – | 0 | 3 | 4 | 19 | 14 | 31 | 24 | 30 | 41 | 35 |
|
|
– | – | – | – | – | 0 | 3 | 18 | 13 | 28 | 22 | 28 | 39 | 33 |
|
|
– | – | – | – | – | – | 0 | 19 | 14 | 29 | 24 | 30 | 41 | 35 |
|
|
– | – | – | – | – | – | – | 0 | 18 | 35 | 34 | 36 | 47 | 42 |
|
|
– | – | – | – | – | – | – | – | 0 | 29 | 28 | 30 | 41 | 39 |
|
|
– | – | – | – | – | – | – | – | – | 0 | 5 | 4 | 14 | 19 |
|
|
– | – | – | – | – | – | – | – | – | – | 0 | 6 | 17 | 18 |
|
|
– | – | – | – | – | – | – | – | – | – | – | 0 | 14 | 19 |
|
|
– | – | – | – | – | – | – | – | – | – | – | – | 0 | 23 |
|
|
– | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Morphometrics of P. honkongensis sp. n. and P. neolucani sp. n.
| Character |
|
Stenostomatous female |
|
Stenostomatous female |
|---|---|---|---|---|
| n | 10 | 10 | 10 | 10 |
| L | 1014 ± 185 (700–1302) | 1181 ± 315 (918–2008) | 871 ± 49.6 (775–951) | 1060 ± 111.1 (930–1297) |
| L’ | 847 ± 184 (560–1125) | 940 ± 288 (703–1700) | 745 ± 50.7 (658–826) | 864 ± 103.2 (743–1080) |
| a | 14 ± 3.7 (11–24) | 13 ± 0.9 (12–15) | 15 ± 1 (14–16) | 14 ± 0.7 (13–15) |
| b | 6 ± 1 (4.6–7.7) | 6.6 ± 1.1 (5.6–9.5) | 5.4 ± 0.6 (4.7–6.6) | 5.5 ± 0.4 (4.8–6.5) |
| c | 6.1 ± 1.3 (5–8.5) | 4.8 ± 0.7 (4.3–6.5) | 7 ± 0.9 (6.1–9.2) | 5.4 ± 0.5 (4.5–6.1) |
| c’ | 4.2 ± 0.9 (3–5.9) | 6.3 ± 0.5 (5.1–6.9) | 3.7 ± 0.7 (2.6–4.9) | 6.1 ± 0.9 (5–7.8) |
| T or V | 53 ± 4.8 (51–68) | 45 ± 2.6 (42–50) | 53 ± 7.5 (33–59) | 47 ± 1.3 (45–48) |
| Maximum body diam. | 73 ± 14.9 (51–96) | 92 ± 29.4 (67–170) | 57 ± 5.8 (49–67) | 75 ± 9 (63–95) |
| Stoma length | 12.6 ± 1 (11.4–14.6) | 13.3 ± 1.6 (10.3–16) | 10.4 ± 1 (8.7–12) | 11 ± 0.9 (10.2–13) |
| Stoma diam. | 7.2 ± 0.7 (6.1–8.5) | 7.7 ±1.1 (6.5–9.7) | 6.0 ± 0.7 (5.3–7.6) | 7.1 ± 0.7 (6.3–8.4) |
| Pharynx length (head to base of pharynx) | 155 ± 9.7 (140–172) | 168 ± 14 (154-202) | 151 ± 11.9 (129-169) | 182 ± 14.9 (149-194) |
| Anterior pharynx (pro- + metacorpus) | 98 ± 5.4 (90–106) | 106 ± 7.4 (94–117) | 95 ± 10.2 (76–112) | 116 ± 9.2 (97–128) |
| Posterior pharynx (isthmus + basal bulb) | 57 ± 5.9 (49–66) | 62 ± 8.4 (56–85) | 56 ± 5.3 (51–69) | 66 ± 7.1 (52–75) |
| Ant/total pharynx % | 63 ± 2 (60–66) | 63 ± 2.5 (58–66) | 63 ± 3.2 (59–67) | 64 ± 1.8 (61– 66) |
| Median bulb diam. | 27 ± 2 (24–31) | 31 ± 3.6 (26–38) | 25 ± 1 (24–27) | 31 ± 2.9 (27–36) |
| Terminal bulb diam. | 26 ± 2.2 (22–28) | 29 ± 6.6 (24–47) | 25 ± 1.3 (22–27) | 29 ± 1.7 (27–33) |
| Testis length | 540 ± 141 (366–765) | – | 467±43 (425–561) | – |
| Ant. end to vulva | – | 539 ± 172 (384–995) | – | 493 ± 50.9 (420–581) |
| Vulva to anus distance | – | 419 ± 149 (320–815) | – | 371 ± 47 (290–447) |
| Cloacal or anal body diam. | 41 ± 5.4 (30–50) | 39 ± 8 (32–60) | 35 ± 4.8 (27–43) | 32 ± 3.8 (27–38) |
| Tail length | 167 ± 21.6 (140–206) | 241 ± 30 (215–308) | 125 ± 13.2 (99–148) | 196 ± 19 (175–228) |
| Spicule length (curve) | 53 ± 4.2 (44–57) | – | 47±2.3 (43–51) | – |
| Spicule length (chord) | 45 ± 3.7 (37–49) | – | 36 ± 1.7 (34–39) | – |
| Gubernaculum length | 19 ± 1.8 (17–22) | – | 17 ± 1.5 (14–19) | – |
Morphometrics of P. riukiariae sp. n. and P. degawai sp. n.
| Character |
|
Stenostomatous female |
|
Stenostomatous female |
|---|---|---|---|---|
| n | 10 | 10 | 10 | 10 |
| L | 918 ± 162 (663–1180) | 1362 ± 211 (1025–1700) | 872 ± 142.2 (710–1105) | 1184 ± 310.5 (811–1853) |
| L’ | 780 ± 151 (553–1010) | 1142 ± 201 (835–1466) | 727 ± 134.6 (585–961) | 986 ± 282 (652–1600) |
| a | 12 ± 2.5 (9.4–15) | 14 ± 1.5 (11–16) | 16 ± 2 (13–19) | 16 ± 3 (132–23) |
| b | 5.7 ± 0.8 (5–7.5) | 8.4 ± 1.5 (5.9–10.8) | 5.3 ± 0.6 (4.6–6.4) | 6.6 ± 1.2 (5.1–8.6) |
| c | 6.8 ± 1.2 (5.4–9.3) | 6.2 ± 0.7 (5–7.3) | 6 ± 0.8 (5.4–7.7) | 6 ± 0.9 (4.7–7.3) |
| c’ | 3.4 ± 0.4 (2.7–4.2) | 5.2 ± 0.6 (4.5–6.1) | 4.1 ± 0.5 (3.3–4.7) | 5.2 ± 1 (3.8–7.3) |
| T or V | 51 ± 5.3 (41–59) | 45 ± 11.1 (32–72) | 45 ± 5 (40–56) | 51 ± 5.5 (45–63) |
| Maximum body diam. | 78 ± 13.2 (62–97) | 99 ± 19.4 (65–128) | 56 ± 14.4 (40–80) | 80 ± 28.6 (37–138) |
| Stoma length | 11.1 ± 1 (9.7–12.5) | 11.8 ± 1.3 (9.2–14) | 11.4 ± 0.8 (10–12.3) | 12.1 ± 0.9 (11–13.4) |
| Stoma diam. | 6.5 ± 0.8 (5.4–7.6) | 7.3 ±0.7 (6.2–8.4) | 6.7 ± 0.3 (6.3–7) | 7.6 ± 0.6 (6.9–8.7) |
| Pharynx length (head to base of pharynx) | 143 ± 17.6 (111–167) | 156 ± 12.2 (136–178) | 152 ± 10.1 (143–176) | 169 ± 16.3 (148–207) |
| Anterior pharynx (pro– + metacorpus) | 94 ± 11 (78–111) | 97 ± 8.8 (86–116) | 95 ± 4.2 (89–103) | 106 ± 8.4 (96–122) |
| Posterior pharynx (isthmus + basal bulb) | 54 ± 5.8 (43–63) | 58 ± 7 (40–66) | 58 ± 7.1 (47–73) | 62 ± 9.6 (51–85) |
| Ant/total pharynx % | 67 ± 11.9 (59–100) | 62 ± 3.4 (60–71) | 62 ± 2.4 (59–67) | 64 ± 2.3 (60– 68) |
| Median bulb diam. | 27 ± 2.2 (23–31) | 31 ± 1.4 (29–33) | 26 ± 3 (22–32) | 30 ± 4.6 (24–41) |
| Terminal bulb diam. | 24 ± 2.8 (19–28) | 28 ± 2.3 (24–32) | 25 ± 4 (19–33) | 28 ± 4.8 (22–39) |
| Testis length | 468 ± 116.4 (295–677) | – | 397 ± 105.7 (285–580) | – |
| Ant. end to vulva | – | 624 ± 199 (415–1040) | – | 601 ± 173.3 (416–1043) |
| Vulva to anus distance | – | 510 ± 94.6 (332–665) | – | 365 ± 72.8 (255–487) |
| Cloacal or anal body diam. | 41 ± 4.5 (32–46) | 43 ± 6.1 (31–52) | 35 ± 5.6 (30–44) | 39 ± 8.7 (22–55) |
| Tail length | 137 ± 21.2 (104–170) | 220 ± 14.3 (190–241) | 145 ± 14.5 (125–180) | 198 ± 36.8 (150–253) |
| Spicule length (curve) | 53 ± 5.5 (40–60) | – | 47±7.6 (37–57) | – |
| Spicule length (chord) | 43 ± 3.4 (36–47) | – | 40 ± 6.9 (32–50) | – |
| Gubernaculum length | 12 ± 2.5 (9.4–15) | – | 16 ± 2.4 (13–19) | – |
Morphometrics of P. laevicollis sp. n.
| Character |
|
Stenostomatous female |
|---|---|---|
| n | 10 | 10 |
| L | 834 ± 104 (662–971) | 1104 ± 209 (746–1325) |
| L’ | 705 ± 89.6 (566–836) | 908 ± 179 (595–1126) |
| a | 15 ± 1 (13–17) | 13 ± 0.8 (12–15) |
| b | 6.5 ± 0.5 (5.9–7.4) | 7.6 ± 0.8 (5.8–8.6) |
| c | 6.5 ± 0.4 (5.9–7.2) | 5.7 ± 0.7 (4.6–6.9) |
| c’ | 3.9 ± 0.4 (3–4.3) | 5.8 ± 0.7 (4.9–7.1) |
| T or V | 59 ± 2 (56–62) | 46 ± 2.1 (42–49) |
| Maximum body diam. | 57 ± 7.4 (47–66) | 83 ± 14.8 (57–97) |
| Stoma length | 9.4 ± 1 (7.7–10.6) | 11.1 ± 1.1 (9–12.5) |
| Stoma diam. | 5.6 ± 0.7 (4–6.4) | 6.4 ± 0.7 (5.5–8.0) |
| Pharynx length (head to base of pharynx) | 118 ± 9.5 (103–132) | 137 ± 15.5 (113–154) |
| Anterior pharynx (pro– + metacorpus) | 72 ± 6.7 (61–81) | 84 ± 8.7 (71–93) |
| Posterior pharynx (isthmus + basal bulb) | 46 ± 2.9 (42–51) | 53 ± 7.1 (42–63) |
| Ant/total pharynx % | 61 ± 1 (58–61) | 61 ± 1.2 (59–63) |
| Median bulb diam. | 20 ± 2.1 (16–22) | 27 ± 3.1 (22–30) |
| Terminal bulb diam. | 19 ± 1.7 (16–21) | 25 ± 3.8 (19–31) |
| Testis length | 490 ± 72.5 (370–570) | – |
| Ant. end to vulva | – | 504±86.2 (365–613) |
| Vulva to anus distance | – | 410 ± 78.9 (275–523) |
| Cloacal or anal body diam. | 33 ± 2.3 (28–36) | 34 ± 4.5 (32–60) |
| Tail length | 129 ± 17.4 (96–152) | 196 ± 47.1 (138–285) |
| Spicule length (curve) | 44 ± 2.5 (40–47) | – |
| Spicule length (chord) | 35 ± 2.1 (31–38) | – |
| Gubernaculum length | 16 ± 1 (14–17) | – |
Summary of diagnostic characters of the Pristionchus pacificus complex sensu stricto.
| Stenostomatous form | Eurystomatous form | ||||||
|---|---|---|---|---|---|---|---|
| Species | Reproductive mode | Right subventral plate | Left subventral plate | Right subventral tooth | Left subventral cusps | Genital papillae | Other characteristic feature(s) |
|
|
H | Single peak | Three bumps | Claw-like | Three large cusps each sometimes split into two (= 3-6 peaks) | Type 1: v1-v2d = v2d-v4; v2d and v3 are close to each other | |
|
|
G | Single peak | Three bumps | Claw-like | three large cusps each sometimes split into two (= 3-6 peaks) | Type 1: v1-v2d = v2d-v4; v2d and v3 are close to each other | |
|
|
G | single peak | three bumps | claw-like | Three large cusps each sometimes split into two to three (= 3-9 peaks) | Type 1: v1-v2d = v2d-v4; v2d and v3 are close to each other | |
|
|
G | Single peak | Three bumps | Claw-like with or without blunt projection | Three large cusps each sometimes split into two (= 3-6 peaks) | Type 1: v1-v2d = v2d-v4; v2d and v3 are close to each other | |
|
|
G | Single peak | Three bumps | Claw-like | Three large cusps each sometimes split into two (= 3-6 peaks) | Type 2: v1-v2d > v2d-v4; v2d and v3 are close to each other | |
|
|
G | Single peak | Three bumps | Claw-like | Three large cusps each sometimes split into two (= 3-6 peaks) | Type 1: v1-v2d = v2d-v4; v2d and v3 are close to each other | |
|
|
G | Single peak | Three bumps | Claw-like with blunt projection | Three large cusps each sometimes split into two (= 3-6 peaks) | Type 1: v1-v2d = v2d-v4; v2d and v3 are close to each other | |
|
|
G | Single peak | Three bumps | Claw-like | Three large cusps each sometimes split into two (= 3-6 peaks) | Type 3: v1-v2 = v2-v4; v2 and v3d are separated | |
|
|
G | 1-2 peaks | with minute denticles | claw-like | three large cusps each sometimes split into two (= 3-6 peaks) | Type 3: v1-v2 = v2-v4; v2 and v3d are separated | |
|
|
G | Single peak | Three bumps | Claw-like | Three large cusps each sometimes split into two (= 3-6 peaks) | Type 4: v1-v2 < v2-v4; v2 and v3d are separated | |
|
|
G | Two peaks | Three bumps | Two large ridges or multiple small denticles | Three large cusps each sometimes split into two to three (= 3-9 peaks) | Type 1: v1-v2d = v2d-v4; v2d and v3 are close to each other | |
|
|
G | Single peak | Three bumps | Claw-like | Three large cusps each sometimes split into two (= 3-6 peaks) | Type 1: v1-v2d = v2d-v4; v2d and v3 are close to each other | |
|
|
G | Single peak | Three bumps | Claw-like | Three large cusps each sometimes split into two (= 3-6 peaks) | v1-v2d = v2d-v4; v2d and v3 are close to each other | v2 and v3 are very close, and sometimes third pair is directed sublaterally (v3d) |
|
|
G | Single peak | Three bumps | Claw-like | Three large cusps each split into three or more (= more than 9 peaks) | v1-v2 < v2-v4; v2 and v3d are separated | Comparatively large and slightly more barrel-shaped stoma in eurystomatous form |
|
|
G | 1-2 peaks | Three bumps | Claw-like | Three large cusps each sometimes split into two (= 3-6 peaks) | v1-v2 < v2-v4; v2 and v3d are separated | |