<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Cupressus sempervirens</italic> (<italic>C. sempervirens</italic>) belongs to the family Cupressaceae. It is widspread in Northern Africa, Greece, Turkey, North America, Cyprus and Syria. Cupressuflavone is the major ingredient of the plant leave extract. The aim of the present study was to evaluate the antiulcerogenic activity of the extract of <italic>C. sempervirens</italic> leaves in gastric ulcer tissues induced by indomethacin. The results of the present study revealed that indomethacin significantly decreased glutathione S-transferase (GST), glutathione peroxidase (GPx), catalase (CAT), reduced glutathione (GSH), glutathione reductase (GR) and superoxide dismutase (SOD) levels, while it increased significantly lipid peroxidation (MDA), nitric oxide (NO) and protein carbonyl (PC) levels in gastric tissue. Furthermore, indomethacin decreased <italic>p53</italic> expression, while it increased bcl-2 expression in gastric tissue. Pretreatment with 5%, 10% &amp; 20% of the LD<sub>50</sub> of <italic>C. sempervirens</italic> and cupressuflavone of indomethacin-treated rats restored all the above parameters to approach normal values. <italic>C. sempervirens</italic> at the highest dose was more effective than the two lower doses. <italic>C. sempervirens</italic> proved more potent than cupressuflavone. In conclusion, <italic>C. sempervirens</italic> exerted antiulcerogenic activity and the effect was dose-dependent and related to the cupressuflavone ingredient of the plant leave extract.</p></abstract>

Cupressus sempervirens (C. sempervirens) belongs to the family Cupressaceae. It is widspread in Northern Africa, Greece, Turkey, North America, Cyprus and Syria. Cupressuflavone is the major ingredient of the plant leave extract. The aim of the present study was to evaluate the antiulcerogenic activity of the extract of C. sempervirens leaves in gastric ulcer tissues induced by indomethacin. The results of the present study revealed that indomethacin significantly decreased glutathione S-transferase (GST), glutathione peroxidase (GPx), catalase (CAT), reduced glutathione (GSH), glutathione reductase (GR) and superoxide dismutase (SOD) levels, while it increased significantly lipid peroxidation (MDA), nitric oxide (NO) and protein carbonyl (PC) levels in gastric tissue. Furthermore, indomethacin decreased p53 expression, while it increased bcl-2 expression in gastric tissue. Pretreatment with 5%, 10% & 20% of the LD50 of C. sempervirens and cupressuflavone of indomethacin-treated rats restored all the above parameters to approach normal values. C. sempervirens at the highest dose was more effective than the two lower doses. C. sempervirens proved more potent than cupressuflavone. In conclusion, C. sempervirens exerted antiulcerogenic activity and the effect was dose-dependent and related to the cupressuflavone ingredient of the plant leave extract.

Protective effect of <italic>Cupressus sempervirens</italic> extract against indomethacin-induced gastric ulcer in rats

Medical Physiology Department, Medical Research Division, National Research Centre, Dokki, Giza, Egypt

Integrative Medicine Cluster, Advanced Medical and Dental Institute (AMDI), University Sains Malaysia (USM), Malaysia

Department of Pathology, Faculty of Medicine, Ain Shams University, Egypt

Faculty of Pharmacy, Cairo University, Egypt

Interdisciplinary Toxicology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Protective effect of  extract against indomethacin-induced gastric ulcer in rats

Cupressus sempervirens (C. sempervirens) belongs to the family Cupressaceae. It is widspread in Northern Africa, Greece, Turkey, North America, Cyprus and...

Protective effect of Cupressus sempervirens extract against indomethacin-induced gastric ulcer in rats

Publié en ligne: 10 déc. 2015

Pages: 25 - 34

Reçu: 14 juil. 2014

Accepté: 10 déc. 2014

DOI: https://doi.org/10.1515/intox-2015-0006

Mots clés
gastric ulcer, Cupressuflavone, antioxidants, apoptosis

© 2015 Interdisciplinary Toxicology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Protective effect of Cupressus sempervirens extract against indomethacin-induced gastric ulcer in rats

Publié en ligne: 10 déc. 2015

Pages: 25 - 34

Reçu: 14 juil. 2014

Accepté: 10 déc. 2014

DOI: https://doi.org/10.1515/intox-2015-0006

Mots clésgastric ulcer, Cupressuflavone, antioxidants, apoptosis

© 2015 Interdisciplinary Toxicology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
gastric ulcer, Cupressuflavone, antioxidants, apoptosis