<abstract xmlns="http://www.w3.org/1999/xhtml"><p>In order to investigate the association between polymorphisms in genes encoding metabolizing enzymes (<italic>CYP1A1</italic>-<italic>Msp</italic>I, <italic>EC-SOD</italic> (<italic>extracellular superoxide dismutase</italic>), <italic>GSTT1</italic>, <italic>GSTM1</italic>, <italic>ALDH2)</italic>, cigarette and alcohol consumption, and the risk of oral squamous cell carcinoma, we conducted a prospective case-control study comprised of 750 individuals with oral squamous cell carcinoma (OSCC) and 750 healthy individuals. Data about smoking and drinking habits were collected along with other demographic and clinical information. Peripheral blood samples were collected for DNA extraction, and polymerase chain reaction (PCR) and PCR-RFLP (restriction fragment length polymorphism) were used to determine genotypes of <italic>CYP1A1</italic>, <italic>EC-SOD, GSTT1</italic>, <italic>GSTM1</italic>, <italic>ALDH2</italic>. The results showed that smoking and alcohol consumption were significantly more common among patients than controls (<italic>p</italic> &lt;0.05). There were significant differences in the genotype distribution for each locus between groups, with the <italic>CYP1A1</italic> (m2/ m2), <italic>EC-SOD</italic> (C/G), <italic>GSTT1</italic> [–], <italic>GSTM1</italic> [–] and <italic>ALDH2</italic> (non G/G) genotypes being more common among patients (<italic>p</italic> &lt;0.05). Furthermore, the majority of patients had at least two or more variant genotypes, while controls had one or no variant genotype (<italic>p</italic> &lt;0.05). Finally, multiple variant genotypes combined with smoking, drinking, or both smoking and drinking significantly increased the risk of OSCC, with greater increase for heavier smoking/drinking. In brief, genetic polymorphism of <italic>CYP1A1</italic>, <italic>EC-SOD</italic>, <italic>GSTT1</italic>, <italic>GSTM1</italic>, and <italic>ALDH2</italic> and smoking and drinking history are closely associated with susceptibility to OSCC.</p></abstract>

In order to investigate the association between polymorphisms in genes encoding metabolizing enzymes (CYP1A1-MspI, EC-SOD (extracellular superoxide dismutase), GSTT1, GSTM1, ALDH2), cigarette and alcohol consumption, and the risk of oral squamous cell carcinoma, we conducted a prospective case-control study comprised of 750 individuals with oral squamous cell carcinoma (OSCC) and 750 healthy individuals. Data about smoking and drinking habits were collected along with other demographic and clinical information. Peripheral blood samples were collected for DNA extraction, and polymerase chain reaction (PCR) and PCR-RFLP (restriction fragment length polymorphism) were used to determine genotypes of CYP1A1, EC-SOD, GSTT1, GSTM1, ALDH2. The results showed that smoking and alcohol consumption were significantly more common among patients than controls (p <0.05). There were significant differences in the genotype distribution for each locus between groups, with the CYP1A1 (m2/ m2), EC-SOD (C/G), GSTT1 [–], GSTM1 [–] and ALDH2 (non G/G) genotypes being more common among patients (p <0.05). Furthermore, the majority of patients had at least two or more variant genotypes, while controls had one or no variant genotype (p <0.05). Finally, multiple variant genotypes combined with smoking, drinking, or both smoking and drinking significantly increased the risk of OSCC, with greater increase for heavier smoking/drinking. In brief, genetic polymorphism of CYP1A1, EC-SOD, GSTT1, GSTM1, and ALDH2 and smoking and drinking history are closely associated with susceptibility to OSCC.

Susceptibility to oral squamous cell carcinoma: correlation with variants of <italic>CYP1A1-Msp</italic>I, <italic>GSTT1, GSTM1, ALDH2, EC-SOD and Lifestyle factors</italic>

Balkan Journal of Medical Genetics

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Susceptibility to oral squamous cell carcinoma: correlation with variants of I, 

In order to investigate the association between polymorphisms in genes encoding metabolizing enzymes (CYP1A1-MspI, EC-SOD (extracellular superoxide dismutase),...

Susceptibility to oral squamous cell carcinoma: correlation with variants of CYP1A1-MspI, GSTT1, GSTM1, ALDH2, EC-SOD and Lifestyle factors

Article Category: Original Article

Publié en ligne: 31 déc. 2016

Pages: 61 - 70

DOI: https://doi.org/10.1515/bjmg-2016-0037

Mots clés
Drinking, genetic polymorphism, oral squamous cell carcinoma (OSCC), smoking

© 2016 Dong T-T, Wang L-J, Liu L-Z, Ma S-N

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Susceptibility to oral squamous cell carcinoma: correlation with variants of CYP1A1-MspI, GSTT1, GSTM1, ALDH2, EC-SOD and Lifestyle factors

Article Category: Original Article

Publié en ligne: 31 déc. 2016

Pages: 61 - 70

DOI: https://doi.org/10.1515/bjmg-2016-0037

Mots clésDrinking, genetic polymorphism, oral squamous cell carcinoma (OSCC), smoking

© 2016 Dong T-T, Wang L-J, Liu L-Z, Ma S-N

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Mots clés
Drinking, genetic polymorphism, oral squamous cell carcinoma (OSCC), smoking