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Asian Biomedicine
Édition 13 (2019): Edition 5 (October 2019)
Accès libre
Expression of autophagic and ubiquitin–proteasome proteins in the peripheral nervous system after nerve injury
Sithiporn Agthong
Sithiporn Agthong
,
Marisa Rodtayoy
Marisa Rodtayoy
et
Atitaya Roumwong
Atitaya Roumwong
| 04 juin 2020
Asian Biomedicine
Édition 13 (2019): Edition 5 (October 2019)
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Article Category:
Brief communication (original)
Publié en ligne:
04 juin 2020
Pages:
173 - 178
DOI:
https://doi.org/10.1515/abm-2019-0057
Mots clés
autophagy
,
nerve injury
,
proteasome
,
ubiquitin
© 2019 Sithiporn Agthong et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.
Figure 1
p62 protein in the right (R) (white bar), left proximal (LP) (light gray bar), and left distal (LD) (dark gray bar) sciatic nerves of rats sacrificed at day 1 (D1), day 3 (D3), day 7 (D7), and day 14 (D14) after nerve crush (n = 7 for each time point). Representative immunoblots are shown above. The bar chart demonstrates the average protein level at each time point. Error bars represent SEM. *P < 0.05 vs. R, #P < 0.01 vs. R.
Figure 2
Beclin-1 protein in the right (R) (white bar), left proximal (LP) (light gray bar), and left distal (LD) (dark gray bar) sciatic nerves of rats sacrificed at day 1 (D1), day 3 (D3), day 7 (D7), and day 14 (D14) after nerve crush (n = 7 for each time point). Representative immunoblots are shown above. The bar chart demonstrates the average protein level at each time point. Error bars represent SEM.
Figure 3
p62 protein in the right (white bars) and left (light-gray bars) L4-6 dorsal root ganglia of rats sacrificed at day 1 (D1), day 3 (D3), day 7 (D7), and day 14 (D14) after nerve crush (n = 7 for each time point). Representative immunoblots are shown above. The bar chart demonstrates the average protein level at each time point. Error bars represent SEM.
Figure 4
Beclin-1 protein in the right (R, white bars) and left (L, gray bars) L4-6 dorsal root ganglia of rats sacrificed at day 1 (D1), day 3 (D3), day 7 (D7), and day 14 (D14) after nerve crush (n = 7 for each time point). Representative immunoblots are shown above. The bar chart demonstrates the average protein level at each time point. Error bars represent SEM.
Figure 5
Muscle ring finger-1 protein in the right (R, white bars) and left (L, left bars) L4-6 dorsal root ganglia of rats sacrificed at day 1 (D1), day 3 (D3), day 7 (D7), and day 14 (D14) after nerve crush (n = 7 for each time point). Representative immunoblots are shown above. The bar chart demonstrates the average protein level at each time-point. Error bars represent SEM.
Figure 6
Ubiquitinated protein in the right (R) and left (L) L4-6 dorsal root ganglia of rats sacrificed at day 1 (D1), day 3 (D3), day 7 (D7), and day 14 (D14) after nerve crush (n = 7 for each time point). Representative immunoblots are shown above. The bar chart demonstrates the left/right ratio at each time point. Error bars represent SEM.
Figure 7
Representative immunoblot of dorsal root ganglia from rats sacrificed at day 1 (D1), day 3 (D3), day 7 (D7), and day 14 (D14) after nerve crush, probed for beta-actin.
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