<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>One of the most relevant and pathogenic groups among the rapidly growing mycobacteria (RGM) is <italic>Mycobacterium abscessus</italic> complex (MABC) that includes three subspecies: <italic>M. abscessus</italic> subsp. <italic>abscessus, M. abscessus</italic> subsp. <italic>bolletii</italic>, and <italic>M. abscessus</italic> subsp. <italic>massiliense</italic>. The aim of this study was the analysis of prevalence of MABC among other non-tuberculous mycobacteria isolated from patients in the Malopolska Region of Poland, between 2018 and 2021, as well as determination of their subspecies and molecular mechanisms of resistance to macrolides and aminoglycosides. The incidence of MABC was 5,4% (12/223). Eight strains were classified as <italic>M. abscessus</italic> subsp. <italic>abscessus</italic>, three as <italic>M. abscessus</italic> subsp. <italic>massiliense</italic> and one <italic>M. abscessus</italic> subsp. <italic>bolletii</italic>. Molecular analysis showed resistance to macrolides for eight strains of <italic>M. abscessus</italic> subsp. <italic>abscessus</italic> associated with <italic>erm</italic>(41)T28 gene mutations. One strain of <italic>M. abscessus</italic> subsp. <italic>abscessus</italic> showed resistance to macrolides (two mutations simultaneously: in <italic>erm</italic>(41)T28 and <italic>rrl</italic> genes) and aminoglycosides (point mutation in <italic>rrs</italic> gene). One strain of <italic>M. abscessus</italic> subs. <italic>bolletii</italic> was resistant to macrolides (<italic>erm</italic>(41)T28 mutation), whereas presented no mutations for aminoglycosides. <italic>M. abscessus</italic> subsp. <italic>massiliense</italic> reveal no mutations. High clarithromycin resistance of <italic>M. abscessus</italic>, determines the urgent need for susceptibility-based treatment. Molecular determination of resistance mechanisms to aminoglycosides and macrolides enables fast and accurate targeted treatment implementation.</p>
</abstract>

One of the most relevant and pathogenic groups among the rapidly growing mycobacteria (RGM) is Mycobacterium abscessus complex (MABC) that includes three subspecies: M. abscessus subsp. abscessus, M. abscessus subsp. bolletii, and M. abscessus subsp. massiliense. The aim of this study was the analysis of prevalence of MABC among other non-tuberculous mycobacteria isolated from patients in the Malopolska Region of Poland, between 2018 and 2021, as well as determination of their subspecies and molecular mechanisms of resistance to macrolides and aminoglycosides. The incidence of MABC was 5,4% (12/223). Eight strains were classified as M. abscessus subsp. abscessus, three as M. abscessus subsp. massiliense and one M. abscessus subsp. bolletii. Molecular analysis showed resistance to macrolides for eight strains of M. abscessus subsp. abscessus associated with erm(41)T28 gene mutations. One strain of M. abscessus subsp. abscessus showed resistance to macrolides (two mutations simultaneously: in erm(41)T28 and rrl genes) and aminoglycosides (point mutation in rrs gene). One strain of M. abscessus subs. bolletii was resistant to macrolides (erm(41)T28 mutation), whereas presented no mutations for aminoglycosides. M. abscessus subsp. massiliense reveal no mutations. High clarithromycin resistance of M. abscessus, determines the urgent need for susceptibility-based treatment. Molecular determination of resistance mechanisms to aminoglycosides and macrolides enables fast and accurate targeted treatment implementation.

Molecular Identification of Strains within the <italic>Mycobacterium abscessus</italic> Complex and Determination of Resistance to Macrolides and Aminoglycosides

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Molecular Identification of Strains within the  Complex and Determination of Resistance to Macrolides and Aminoglycosides

One of the most relevant and pathogenic groups among the rapidly growing mycobacteria (RGM) is Mycobacterium abscessus complex (MABC) that includes three...

Molecular Identification of Strains within the Mycobacterium abscessus Complex and Determination of Resistance to Macrolides and Aminoglycosides

Article Category: ORIGINAL PAPER

Publicado en línea: 16 dic 2023

Páginas: 491 - 506

Recibido: 15 sept 2023

Aceptado: 11 nov 2023

DOI: https://doi.org/10.33073/pjm-2023-048

Palabras clave
Mycobacterium abscessus complex, nontuberculous mycobacteria, macrolides, aminoglycosides, molecular identification

© 2023 Katarzyna Kania et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Molecular Identification of Strains within the Mycobacterium abscessus Complex and Determination of Resistance to Macrolides and Aminoglycosides

Article Category: ORIGINAL PAPER

Publicado en línea: 16 dic 2023

Páginas: 491 - 506

Recibido: 15 sept 2023

Aceptado: 11 nov 2023

DOI: https://doi.org/10.33073/pjm-2023-048

Palabras claveMycobacterium abscessus complex, nontuberculous mycobacteria, macrolides, aminoglycosides, molecular identification

© 2023 Katarzyna Kania et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Palabras clave
Mycobacterium abscessus complex, nontuberculous mycobacteria, macrolides, aminoglycosides, molecular identification