Isolation and Characterization of Potential Lignin Peroxidase-Producing Bacteria from Compost Samples at Richards Bay (South Africa)

Sindiswa L. Dube; Foluso O. Osunsanmi; Bongekile P. Ngcobo; Londiwe B. Mkhwanazi; Zanele Z. Jobe; Raphael T. Aruleba; Rebamang A. Mosa; Andrew R. Opoku

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Isolation and Characterization of Potential Lignin Peroxidase-Producing Bacteria from Compost Samples at Richards Bay (South Africa)

| 14 jun 2023

Polish Journal of Microbiology

Volumen 72 (2023): Edición 2 (June 2023)

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Article Category: ORIGINAL PAPER

Publicado en línea: 14 jun 2023

Páginas: 117 - 124

Recibido: 07 nov 2022

Aceptado: 27 feb 2023

DOI: https://doi.org/10.33073/pjm-2023-003

Palabras clave
lignin, bacteria, ligninolytic activity, lignin peroxidase, 16S rDNA

© 2023 Sindiswa L. Dube et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Graphs of lignin degradation capability, pH variation, and growth of E. coli, P. aeruginosa, E. kobei, and E. xiangfangensis.

Enzyme activity (the recovered supernatant) of E. coli (LR0250096), and P. aeruginosa (CP031449.2) on the lignin enriched medium.

The phylogenetic tree of the bacteria from this study (red) in relation to some other ligninolytic bacteria in the NCBI database.

Degradation of lignin model compounds; guaiacol and veratryl alcohols by the bacterial isolates from the compost samples.

S/N	Bacterial code	Isolation source	Guaiacol alcohol	Veratryl alcohol
1	Old A	Old cow dung	++	++
2	*Old A	Old cow dung	++	++
3	OOld A	Old cow dung	++	+++
4	Old A1	Old cow dung	+	+
5	*Old A2	Old cow dung	+	++
6	Old A2	Old cow dung	+++	+++
7	/*New A	Fresh cow dung	–	++
8	New A	Fresh cow dung	+++	+++
9	*New A	Fresh cow dung	–	++
10	New B	Fresh cow dung	++	++
11	ONew B	Fresh cow dung	–	–
12	*New B	Fresh cow dung	–	–
13	Saw A	Sawdust	–	–
14	Saw A1	Sawdust	–	+
15	Saw A2	Sawdust	+++	+++
16	Saw B	Sawdust	–	–
17	*Saw B	Sawdust	–	+++
18	Saw B1	Sawdust	–	++
19	Soil A	Soil	–	+
20	*Soil A	Soil	–	+++
21	Soil A1	Soil	+	++
22	*Soil B	Soil	+	+++
23	Soil B	Soil	++	+++
24	Soil B1	Soil	+++	+++
25	Soil 2	Soil	+	++
26	Soil 7	Soil	+	++

Quantitative analysis for the lignin peroxidase activity of the bacteria isolates.

S/N	Bacterial strains	Lignin peroxidase activity (U/l)
1	Pseudomonas aeruginosa (MF144461.1)	1.37 × 10^–2a
2	Escherichia coli (LR025096.1)	1.99 × 10^–1b
3	Pseudomonas aeruginosa (KR136350.1)	8.70 × 10^–2c
4	Pseudomonas aeruginosa (CP031449.2)	1.99 × 10^–1b
5	Enterobacter xiangfangensis (MH304301.1)	1.09 × 10^–2d
6	Enterobacter kobei (CP032897.1)	8.55 × 10^–2c

Identification of the isolated bacterial cultures by 16S rDNA.

Bacterial name	Accession number (16S rRNA gene sequence)	Bacterial code
Pseudomonas aeruginosa	KR136350.1	Soil B
Pseudomonas aeruginosa	KT799669.1	*Old A
Pseudomonas aeruginosa	MF144461.1	OOld A
Pseudomonas aeruginosa	KR136350.1	New B
Pseudomonas aeruginosa	KR136350.1	New A
Pseudomonas aeruginosa	CP031449.2	Saw A2
Pseudomonas aeruginosa	KT799669.1	Soil B1
Escherichia coli	LR025096.1	Old A2
Enterobacter kobei	CP032897.1	Soil 7
Enterobacter xiangfangensis	MH304301.1	Soil 2

Lignin peroxidase and Laccase producing potential of the isolated bacteria.

S/N	Bacterial code	Lignin peroxidase activity	Laccase activity
1	*Old A	+++	–
2	OOld A	+++	–
3	Old A2	+++	–
4	New A	+++	–
5	New B	+++	–
6	Saw A2	+++	–
7	Soil B	+++	–
8	Soil B1	+++	–
9	Soil 2	++	–
10	Soil 7	++	–

Decolourization of synthetic lignin-like dyes (halo zone measured in mm) by the selected ligninolytic bacterial isolates.

S/N	Bacterial code	RBBR (mm)	AZ (mm)	CR (mm)
1	Old A	15.0 ± 0.10^c	35.0 ± 0.04^b	0.00 ± 0.00^f
2	*Old A	26.0 ± 0.00^b	40.0 ± 0.01^d	42.0 ± 0.01^a
3	OOld A	24.0 ± 0.06^b	30.0 ± 0.00^c	31.0 ± 0.10^c
4	Old A1	19.0 ± 0.00^d	29.0 ± 0.05^c	0.00 ± 0.00^f
5	*Old A2	0.00 ± 0.00^f	34.0 ± 0.04^e	10.0 ± 0.20^d
6	Old A2	35.0 ± 0.10^a	42.0 ± 0.11^d	40.0 ± 0.08^a
7	New A	8.00 ± 0.50^e	40.0 ± 0.00^d	20.0 ± 0.01^e
8	New B	30.0 ± 0.00^h	50.0 ± 0.01^a	26.0 ± 0.02^b
9	Saw A2	15.0 ± 0.04^c	35.0 ± 0.02^b	12.0 ± 0.50^d
10	Soil A1	0.00 ± 0.00^f	8.00 ± 0.01^g	24.0 ± 0.10^b
11	*Soil B	0.00 ± 0.00^f	25.0 ± 0.02^f	19.0 ± 0.00^e
12	Soil B	34.0 ± 0.00^a	50.0 ± 0.02^a	19.0 ± 0.10^e
13	Soil B1	10.0 ± 0.06^e	37.0 ± 0.01^b	40.0 ± 0.03^a
14	Soil 2	49.0 ± 0.00^h	25.0 ± 0.08^f	49.0 ± 0.01^h
15	Soil 7	35.0 ± 0.02^a	16.0 ± 0.04^h	35.0 ± 0.05ⁱ

Characteristics of ligninolytic bacteria from cow dung, sawdust, and soil samples.

Bacterial code	Catalase test	Oxidase test	Starch hydrolysis	Urease test	Indole test	Citrate test
*Old A	+	–	+	–	+	–
OOld A	+	+	+	–	+	–
Old A2	+	+	+	–	+	–
New A	+	+	+	–	+	–
New B	+	+	+	–	+	–
Saw A2	+	–	+	–	+	–
Soil B	+	–	+	–	+	–
Soil B1	+	–	+	–	+	–
Soil 2	+	+	+	–	+	–
Soil 7	+	–	+	–	+	–

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Isolation and Characterization of Potential Lignin Peroxidase-Producing Bacteria from Compost Samples at Richards Bay (South Africa)

Article Category: ORIGINAL PAPER

Publicado en línea: 14 jun 2023

Páginas: 117 - 124

Recibido: 07 nov 2022

Aceptado: 27 feb 2023

DOI: https://doi.org/10.33073/pjm-2023-003

Palabras clave
lignin, bacteria, ligninolytic activity, lignin peroxidase, 16S rDNA

© 2023 Sindiswa L. Dube et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Fig. 3.

Degradation of lignin model compounds; guaiacol and veratryl alcohols by the bacterial isolates from the compost samples.

Quantitative analysis for the lignin peroxidase activity of the bacteria isolates.

Identification of the isolated bacterial cultures by 16S rDNA.

Lignin peroxidase and Laccase producing potential of the isolated bacteria.

Decolourization of synthetic lignin-like dyes (halo zone measured in mm) by the selected ligninolytic bacterial isolates.

Characteristics of ligninolytic bacteria from cow dung, sawdust, and soil samples.

Isolation and Characterization of Potential Lignin Peroxidase-Producing Bacteria from Compost Samples at Richards Bay (South Africa)

Article Category: ORIGINAL PAPER

Publicado en línea: 14 jun 2023

Páginas: 117 - 124

Recibido: 07 nov 2022

Aceptado: 27 feb 2023

DOI: https://doi.org/10.33073/pjm-2023-003

Palabras clavelignin, bacteria, ligninolytic activity, lignin peroxidase, 16S rDNA

© 2023 Sindiswa L. Dube et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Fig. 3.

Degradation of lignin model compounds; guaiacol and veratryl alcohols by the bacterial isolates from the compost samples.

Quantitative analysis for the lignin peroxidase activity of the bacteria isolates.

Identification of the isolated bacterial cultures by 16S rDNA.

Lignin peroxidase and Laccase producing potential of the isolated bacteria.

Decolourization of synthetic lignin-like dyes (halo zone measured in mm) by the selected ligninolytic bacterial isolates.

Characteristics of ligninolytic bacteria from cow dung, sawdust, and soil samples.

Palabras clave
lignin, bacteria, ligninolytic activity, lignin peroxidase, 16S rDNA