<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Listeria monocytogenes</italic> is the etiological factor of listeriosis. The main source of these organisms is food, including dairy products. The aim was to determine the multiple correlations between the drug susceptibility, virulence genes (VGs), and biofilm formation on silicone teat cups of milk-borne and human <italic>L. monocytogenes</italic> strains. The spread of <italic>L. monocytogenes</italic> via contaminated teat rubbers was assessed. The <italic>L. monocytogenes</italic> strains recovered from milk (18), human blood (10), and the reference strain ATCC®19111™ were used in the study. Penicillin resistance was the most prevalent resistance in the milk isolates (n=8; 44.4%), whereas among clinical strains erythromycin resistance was predominating – (n=6; 60%). The most frequent VGs among strains isolated from milk were <italic>hlyA</italic> (100%) and <italic>plcB</italic> (100%) whereas in strains isolated from blood – <italic>hlyA</italic> (100%) and <italic>prfA</italic> (90%). All tested VGs were present in 50% of blood isolates and 11% of milk-borne strains. The strains isolated from milk formed a significantly stronger biofilm. The strains with more numerous virulence genes were resistant to more antibiotics and formed a stronger biofilm. It was shown that contaminated teat cups might contribute to the transmission of <italic>L. monocytogenes</italic> in the herd. It seems reasonable to monitor the occurrence of <italic>L. monocytogenes</italic> biofilm in a dairy processing environment.</p></abstract>

Listeria monocytogenes is the etiological factor of listeriosis. The main source of these organisms is food, including dairy products. The aim was to determine the multiple correlations between the drug susceptibility, virulence genes (VGs), and biofilm formation on silicone teat cups of milk-borne and human L. monocytogenes strains. The spread of L. monocytogenes via contaminated teat rubbers was assessed. The L. monocytogenes strains recovered from milk (18), human blood (10), and the reference strain ATCC®19111™ were used in the study. Penicillin resistance was the most prevalent resistance in the milk isolates (n=8; 44.4%), whereas among clinical strains erythromycin resistance was predominating – (n=6; 60%). The most frequent VGs among strains isolated from milk were hlyA (100%) and plcB (100%) whereas in strains isolated from blood – hlyA (100%) and prfA (90%). All tested VGs were present in 50% of blood isolates and 11% of milk-borne strains. The strains isolated from milk formed a significantly stronger biofilm. The strains with more numerous virulence genes were resistant to more antibiotics and formed a stronger biofilm. It was shown that contaminated teat cups might contribute to the transmission of L. monocytogenes in the herd. It seems reasonable to monitor the occurrence of L. monocytogenes biofilm in a dairy processing environment.

Characteristics of <italic>Listeria monocytogenes</italic> Strains Isolated from Milk and Humans and the Possibility of Milk-Borne Strains Transmission

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Characteristics of  Strains Isolated from Milk and Humans and the Possibility of Milk-Borne Strains Transmission

Listeria monocytogenes is the etiological factor of listeriosis. The main source of these organisms is food, including dairy products. The aim was to determine...

Characteristics of Listeria monocytogenes Strains Isolated from Milk and Humans and the Possibility of Milk-Borne Strains Transmission

Article Category: original-paper

Publicado en línea: 03 sept 2019

Páginas: 353 - 369

Recibido: 25 abr 2019

Aceptado: 23 jul 2019

DOI: https://doi.org/10.33073/pjm-2019-038

Palabras clave
biofilm formation, drug susceptibility, bacteria transmission, virulence genes frequency

© 2019 Anton Pyskun et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Characteristics of Listeria monocytogenes Strains Isolated from Milk and Humans and the Possibility of Milk-Borne Strains Transmission

Article Category: original-paper

Publicado en línea: 03 sept 2019

Páginas: 353 - 369

Recibido: 25 abr 2019

Aceptado: 23 jul 2019

DOI: https://doi.org/10.33073/pjm-2019-038

Palabras clavebiofilm formation, drug susceptibility, bacteria transmission, virulence genes frequency

© 2019 Anton Pyskun et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Palabras clave
biofilm formation, drug susceptibility, bacteria transmission, virulence genes frequency