Profile | Drug resistance/susceptibility | Number of strains | Total (n = 28) | |
---|---|---|---|---|
Strains from milk (n = 18) (the strain identification number) | Strains from the blood (n = 10) (the strain identification number) | |||
A | R: - | 8 (44.44%) | 4 (40.0%) | 12 (42.86%) |
B | R: P | 3 (16.67%) | 0 (0.0%) | 3 (10.71%) |
C | R: E | 1 (5.56%) | 4 (40.0%) | 5 (17.86%) |
D | R: P, E | 1 (5.56%) | 0 (0.0%) | 1 (3.57%) |
E | R: E, SXT | 1 (5.56%) | 0 (0.0%) | 1 (3.57%) |
F | R: P, AM, MEM, SXT | 1 (5.56%) | 0 (0.0%) | 1 (3.57%) |
G | R: P, AM, MEM, E, SXT | 3 (16.67%) | 0 (0.0%) | 3 (10.71%) |
H | R: MEM, E | 0 (0. 0%) | 2 (20.0%) | 2 (7.14%) |
Strains origin | The strain identification number | Mean number of bacteria [log CFU/cm−2] | STD |
---|---|---|---|
Milk | 372 | 6.87 | ± |
Milk | 375 | 6.79 | ± |
Milk | 112 | 6.71 | ± |
Milk | 368 | 6.64 | ± |
Milk | 344 | 6.05 | ± |
Milk | 143 | 5.95 | ± |
Milk | 267 | 5.81 | ± |
Milk | 251 | 5.18 | ± |
Milk | 185 | 4.99 | ± |
Milk | 7m | 4.94 | ± |
Milk | 199 | 4.94 | ± |
Milk | 317 | 4.89 | ± |
Milk | 4m | 4.85 | ± |
Milk | 282 | 4.81 | ± |
Milk | 76 | 4.77 | ± |
Milk | 29 | 4.76 | ± |
Milk | 231 | 4.74 | ± |
Milk | 63 | 4.64 | ± |
ATCC | 19111 | 5.80 | ± |
Blood | 5 | 5.70 | ± |
Blood | 6 | 5.67 | ± |
Blood | 3 | 4.18 | ± |
Blood | 1 | 4.01 | ± |
Blood | 8 | 4.00 | ± |
Blood | 2 | 3.97 | ± |
Blood | 9 | 3.92 | ± |
Blood | 7 | 3.72 | ± |
Blood | 10 | 3.71 | ± |
Blood | 4 | 3.61 | ± |
Strain | Number of re-isolated | ||||||||
---|---|---|---|---|---|---|---|---|---|
Silicone teat cup | Udder skin | Udder skin | |||||||
Sample 1 | Sample 2 | Sample 3 | Sample 4 | Sample 5 | Sample 6 | Recovery of bacteria (12 h/25°C) | |||
M372 | 5.91 | 3.81 | 3.77 | 3,. 0 | 3.00 | 2.81 | 2.81 | 1.92 | 2.83 |
M375 | 5.84 | 3.68 | 3.61 | 2.68 | 2.68 | 1.87 | 1.87 | n.d. | 1.66 |
M231 | 5.57 | 3.71 | 3.66 | 2.68 | 2.68 | 2.00 | 2.00 | 1.11 | 2.37 |
M63 | 5.52 | 3.54 | 3.47 | 1.47 | 1.47 | 1.00 | 1.00 | n.d | 1.26 |
ATCC 19111 | 5.66 | 3.76 | 3.72 | 2.74 | 2.74 | 2.26 | 2.26 | 1.64 | 2.57 |
B5 | 5.71 | 3.62 | 3.56 | 2.57 | 2.57 | 1.84 | 1.84 | n.d. | 1.46 |
B6 | 5.69 | 3.79 | 3.73 | 2.91 | 2.91 | 2.72 | 2.72 | 1.81 | 2.72 |
B10 | 5.32 | 3.59 | 3.53 | 1.62 | 1.62 | 1.11 | 1.11 | n.d. | 1.20 |
B4 | 5.13 | 3.58 | 3.51 | 1.52 | 1.52 | 0.90 | 0.90 | n.d. | 1.08 |
Primer Primer sequence (5' - 3') | Size of PCR product (bp) | Amplification conditions |
---|---|---|
L1 CAG CAG CCG CGG TAA TAC | 938 | Initial denaturation - 94°C/2 min 30 cycles: denaturation - 94°C/30 s annealing, - 50°C/30 s elongation - 72°C/1 min final elongation - 72°C/5 min |
L2 CTC CAT AAA GGT GAC CCT | ||
LM1 CCT AAG ACG CCA ATC GAA | 750 | |
LM2 AAG CAC TTG CAA CTG CTC |
Strain | Number of re-isolated | |
---|---|---|
Silicone teat cup | Milk | |
M372 | 6.87 | 3.37 |
M375 | 6.79 | 3.29 |
M231 | 4.74 | 2.75 |
M63 | 4.64 | 2.71 |
ATCC 19111 | 5.80 | 2.97 |
B5 | 5.70 | 2.94 |
B6 | 5.67 | 2.84 |
B10 | 3.71 | 2.63 |
B4 | 3.61 | 2.57 |
Profile | Virulence genes | Number of strains | The strain identification number |
---|---|---|---|
Strains isolated from milk | |||
I | 2 | 4, 63 | |
II | 2 | 7, 29 | |
III | 2 | 199, 231 | |
IV | 2 | 282, 317 | |
V | 2 | 76, 185 | |
VI | 2 | 143,368 | |
VII | 2 | 372, 375 | |
VIII | 1 | 267 | |
IX | 1 | 344 | |
X | 1 | 251 | |
XI | 1 | 112 | |
Strains isolated from the blood | |||
I | 5 | 1, 2, 5, 6, 9 | |
II | 1 | 4 | |
III | 1 | 10 | |
IV | 1 | 3 | |
V | 1 | 8 | |
VI | 1 | 7 |
Strain | Number of re-isolated | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Silicone teat cup | Udder skin | |||||||||||
Sample 1 | Sample 2 | Sample 3 | Sample 4 | Sample 5 | Sample 6 | Sample 7 | Sample 8 | Regrowth on first negative or last positive sample (12 h/25°C) | ||||
M372 | 6.87 | 4.33 | 3.88 | 3.71 | 3.45 | 2.92 | 2.66 | 2.09 | 1.85 | 2.92 | ||
M375 | 6.79 | 4.54 | 3.99 | 3.65 | 3.10 | 2.83 | 2.51 | 1.92 | 1.61 | 2.79 | ||
M231 | 4.74 | 3.87 | 3.76 | 3.47 | 2.79 | 2.52 | 1.93 | 1.67 | 1.08 | 1.99 | ||
M63 | 4.64 | 3.96 | 3.80 | 3.34 | 2.70 | 2.33 | 1.86 | 1.59 | n.d. | 1.87 | ||
ATCC 19111 | 5.80 | 3.64 | 3.02 | 2.79 | 2.62 | 2.14 | 1.79 | 1.53 | n.d. | 2.01 | ||
B5 | 5.70 | 3.56 | 2.85 | 2.63 | 2.48 | 1.96 | 1.76 | 1.43 | n.d. | 1.81 | ||
B6 | 5.67 | 3.47 | 2.91 | 2.76 | 2.39 | 1.92 | 1.64 | 1.26 | n.d. | 1.72 | ||
B10 | 3.71 | 2.85 | 2.57 | 1.88 | 1.49 | n.d. | n.d. | n.d. | n.d. | 1.63 | ||
B4 | 3.61 | 2.92 | 2.73 | 1.84 | 1.20 | n.d. | n.d. | n.d. | n.d. | 1.38 |
Primer | Primer sequence (5’ - 3’) | Size of PCR product (bp) |
---|---|---|
CGC CGC GGA AAT TAA AAA AAG A | 839 | |
ACG AAG GAA CCG GGC TGC TAG | ||
GCA GTT GCA AGC GCT TGG AGT GAA | 456 | |
GCA ACG TAT CCT CCA GAG TGA TCG | ||
ACA AGC TGC ACC TGT TGC AG | 131 | |
TGA CAG CGT GTG TAG TAG CA | ||
CAG GCA GCT ACA ATT ACA CA | 2 341 | |
ATA TAG TCC GAA AAC CAC ATC T | ||
AGG AGA GGA TAG TGT GAA | 1 905 | |
TTA TTT CTG TGC CCT TAA | ||
CTG CTT GAG CGT TCA TGT CTC ATC CCC C | 1 484 | |
ATG GGT TTC ACT CTC CTT CTA C | ||
GCA AGT GTT CTA GTC TTT CCG G | 794 | |
ACC TGC CAA AGT TTG CTG TGA | ||
CAT GAA CGC TCA AGC AGA AG | 706 | |
AAT TTT CCC AAG TAG CAG GA |