<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>The environmental conditions in Madeira Island are favorable for the presence and dissemination of the pinewood nematode (PWN), Bursaphelenchus xylophilus. Five hundred Pinus pinaster wood samples were collected in several forest areas and PWN was detected in 22.8 % of the samples. Bursaphelenchus xylophilus isolates from Madeira Island displayed the species-specific diagnostic characters. A morphological variation in the female tail terminus was detected. In most females, the tail presented a broadly rounded terminus and, occasionally, a digitate terminus with a terminal nipple-like extension resembling a mucro. PCR ITS-RFLP analysis revealed that Madeira Island isolates exhibited patterns specific to the species B. xylophilus and similar to virulent isolates. Amplified ITS regions were further sequenced and no genetic diversity was found for this genomic region among 17 Portuguese isolates (Madeira Island and Continental Portugal). Phylogenetic analysis revealed that Portuguese isolates grouped with isolates from China, Korea and one isolate from Japan.</p></abstract>

The environmental conditions in Madeira Island are favorable for the presence and dissemination of the pinewood nematode (PWN), Bursaphelenchus xylophilus. Five hundred Pinus pinaster wood samples were collected in several forest areas and PWN was detected in 22.8 % of the samples. Bursaphelenchus xylophilus isolates from Madeira Island displayed the species-specific diagnostic characters. A morphological variation in the female tail terminus was detected. In most females, the tail presented a broadly rounded terminus and, occasionally, a digitate terminus with a terminal nipple-like extension resembling a mucro. PCR ITS-RFLP analysis revealed that Madeira Island isolates exhibited patterns specific to the species B. xylophilus and similar to virulent isolates. Amplified ITS regions were further sequenced and no genetic diversity was found for this genomic region among 17 Portuguese isolates (Madeira Island and Continental Portugal). Phylogenetic analysis revealed that Portuguese isolates grouped with isolates from China, Korea and one isolate from Japan.

The pinewood nematode, Bursaphelenchus xylophilus, in Madeira Island

IMAR-CMA, Department of Life Sciences, University of Coimbra, 3004-517, Coimbra, Portugal

Laboratório de Qualidade Agrícola, RAM, 9135-372, Santa Cruz, Portugal

Direcção Regional de Florestas, 9020-149, Funchal, Portugal

NemaLab-ICAAM, University of Évora, 7002-554, Évora, Portugal

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"The pinewood nematode, Bursaphelenchus xylophilus, in Madeira Island"}

The environmental conditions in Madeira Island are favorable for the presence and dissemination of the pinewood nematode (PWN), Bursaphelenchus xylophilus....

The pinewood nematode, Bursaphelenchus xylophilus, in Madeira Island

Publicado en línea: 11 abr 2012

Páginas: 96 - 103

DOI: https://doi.org/10.2478/s11687-012-0020-3

Palabras clave
Bursaphelenchus xylophilus, Madeira Island, morphology, pinewood nematode, ITS-RFLP, phylogeny

© 2012 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The pinewood nematode, Bursaphelenchus xylophilus, in Madeira Island

Publicado en línea: 11 abr 2012

Páginas: 96 - 103

DOI: https://doi.org/10.2478/s11687-012-0020-3

Palabras claveBursaphelenchus xylophilus, Madeira Island, morphology, pinewood nematode, ITS-RFLP, phylogeny

© 2012 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
Bursaphelenchus xylophilus, Madeira Island, morphology, pinewood nematode, ITS-RFLP, phylogeny