<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>Identification of the nematode Xiphinema italiae relies mainly on time-consuming morphological and morphometrical studies. A polymerase chain reaction protocol has been used for the reliable and specific identification of X. italiae. Moreover, four independently evolving molecular markers (cox1- cytochrome c oxidase subunit 1; ITS2-second internal transcribed spacer; 18S gene and D2/D3 expansion segments of 28S gene) were amplified and sequenced in both directions.</p></abstract>

Identification of the nematode Xiphinema italiae relies mainly on time-consuming morphological and morphometrical studies. A polymerase chain reaction protocol has been used for the reliable and specific identification of X. italiae. Moreover, four independently evolving molecular markers (cox1- cytochrome c oxidase subunit 1; ITS2-second internal transcribed spacer; 18S gene and D2/D3 expansion segments of 28S gene) were amplified and sequenced in both directions.

Molecular confirmation of Xiphinema italiae Meyl, 1953 (Nematoda: Longidoridae) from the Slovak Republic

Division of Plant Health, Crop Research Institute, Drnovská 507, Ruzyně, 16106, Prague 6, Czech Republic

Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 040 01, Košice, Slovak Republic

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Identification of the nematode Xiphinema italiae relies mainly on time-consuming morphological and morphometrical studies. A polymerase chain reaction protocol...

Molecular confirmation of Xiphinema italiae Meyl, 1953 (Nematoda: Longidoridae) from the Slovak Republic

Publicado en línea: 21 jun 2009

Páginas: 131 - 134

DOI: https://doi.org/10.2478/s11687-009-0025-8

Palabras clave
Xiphinema italiae, PCR, ITS2, cox1, 18S, 28S

© 2009 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Molecular confirmation of Xiphinema italiae Meyl, 1953 (Nematoda: Longidoridae) from the Slovak Republic

Publicado en línea: 21 jun 2009

Páginas: 131 - 134

DOI: https://doi.org/10.2478/s11687-009-0025-8

Palabras claveXiphinema italiae, PCR, ITS2, cox1, 18S, 28S

© 2009 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
Xiphinema italiae, PCR, ITS2, cox1, 18S, 28S