Acceso abierto

Antimicrobial activity, viability, and physicochemical properties of an MTA-type cement with different concentrations of bismuth trioxide

Miryam Martínez-Hernández

Miryam Martínez-Hernández

Biointerphases Laboratory, Postgraduate Studies, and Research Division. Faculty of Dentistry, National Autonomous University of México. Circuito exterior s/n, Ciudad UniversitariaCDMX, México
Buscar este autor en
Sciendo|Google Scholar
Martínez-Hernández, Miryam
, 
Daniel Manzo-García

Daniel Manzo-García

Dental Materials and Biomaterials Research Laboratory, Postgraduate Studies, and Research Division. Faculty of Dentistry, National Autonomous University of México, Circuito de la Investigación CientíficaCDMX, México
Buscar este autor en
Sciendo|Google Scholar
Manzo-García, Daniel
, 
Juana Paulina Ramírez-Ortega

Juana Paulina Ramírez-Ortega

Dental Materials and Biomaterials Research Laboratory, Postgraduate Studies, and Research Division. Faculty of Dentistry, National Autonomous University of México, Circuito de la Investigación CientíficaCDMX, México
Buscar este autor en
Sciendo|Google Scholar
Ramírez-Ortega, Juana Paulina
, 
Juan Antonio Arreguín-Cano

Juan Antonio Arreguín-Cano

Departement of Health of the State of Guerrero, Chilpancingo de los BravoGuerrero, México
Buscar este autor en
Sciendo|Google Scholar
Arreguín-Cano, Juan Antonio
, 
Elda Georgina Chávez-Cortéz

Elda Georgina Chávez-Cortéz

School of Dentistry Mexicali, Autonomous University of Baja CaliforniaMexicali, México
Buscar este autor en
Sciendo|Google Scholar
Chávez-Cortéz, Elda Georgina
, 
Jesús Ángel Arenas-Alatorre

Jesús Ángel Arenas-Alatorre

Central Microscopy Laboratory, Institute of Physics, National Autonomous University of MexicoMexico City, México
Buscar este autor en
Sciendo|Google Scholar
Arenas-Alatorre, Jesús Ángel
, 
Jacqueline Adelina Rodríguez-Chávez

Jacqueline Adelina Rodríguez-Chávez

Department of Integral Dental Clinics, University Center for Health Sciences. University of GuadalajaraGuadalajara, México
Buscar este autor en
Sciendo|Google Scholar
Rodríguez-Chávez, Jacqueline Adelina
 y 
Abigailt Flores-Ledesma

Abigailt Flores-Ledesma

Dental Materials and Biomaterials Laboratory, Faculty of Stomatology, Meritorious Autonomous University of PueblaPuebla, México
Buscar este autor en
Sciendo|Google Scholar
Flores-Ledesma, Abigailt
  
20 nov 2023
Antimicrobial activity, viability, and physicochemical properties of an MTA-type cement with different concentrations of bismuth trioxide's Cover Image
Acerca de este artículo
Artículo anterior
Artículo siguiente
Cite
Descargar portada
Publicado en línea: 20 nov 2023
Páginas: 339 - 353
Recibido: 04 sept 2023
Aceptado: 02 oct 2023
DOI: https://doi.org/10.2478/msp-2023-0026
Palabras clave
© 2023 Miryam Martínez-Hernández et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

(A) Evaluation of the antibacterial effect of the experimental cements: Bi15, Bi20, and Bi25, on S. mutans. Positive control: 0.20% chlorhexidine (ANOVA p < 0.05; post hoc Tukey test p < 0.01). 0.20% CHX was significant against all experimental groups (ANOVA p < 0.01; post hoc Dunnett test p < 0.05). Lowercase letters are used to compare means; means sharing a superscript letter are not significantly different, n = 3. (B) Evaluation of the antimicrobial effect of the experimental cements: Bi15, Bi20, and Bi25 on P. gingivalis. Positive control: 0.20% chlorhexidine solution. (ANOVA p < 0.01; post hoc Tukey test p < 0.05). 0.20% CHX was significant against the experimental groups Bi20 and Bi25 (ANOVA p < 0.05; post hoc Dunnett test p < 0.05). Lowercase letters are used to compare means; means sharing a superscript letter are not significantly different, n = 3
(A) Evaluation of the antibacterial effect of the experimental cements: Bi15, Bi20, and Bi25, on S. mutans. Positive control: 0.20% chlorhexidine (ANOVA p < 0.05; post hoc Tukey test p < 0.01). 0.20% CHX was significant against all experimental groups (ANOVA p < 0.01; post hoc Dunnett test p < 0.05). Lowercase letters are used to compare means; means sharing a superscript letter are not significantly different, n = 3. (B) Evaluation of the antimicrobial effect of the experimental cements: Bi15, Bi20, and Bi25 on P. gingivalis. Positive control: 0.20% chlorhexidine solution. (ANOVA p < 0.01; post hoc Tukey test p < 0.05). 0.20% CHX was significant against the experimental groups Bi20 and Bi25 (ANOVA p < 0.05; post hoc Dunnett test p < 0.05). Lowercase letters are used to compare means; means sharing a superscript letter are not significantly different, n = 3

Fig. 2.

Effect of bismuth on cell viability of Bi15, Bi20, and Bi25 cements. L929 cells were seeded in 96-well plates (2.5 × 103 cells/well) and treated with different percentages of bismuth followed by MTT assay for 24, 48, and 72 h. The red horizontal dotted line represents the basal group as 100%, and a blue continuous line represents the threshold for a cytotoxicity level of 75% of cell viability compared to the basal group (negative control). Each bar represents mean ± SD calculated from three independent experiments. ANOVA p < 0.01; post hoc Tukey test p < 0.05, lowercase letters are used to compare means; means sharing a superscript letter are not significantly different, n = 3
Effect of bismuth on cell viability of Bi15, Bi20, and Bi25 cements. L929 cells were seeded in 96-well plates (2.5 × 103 cells/well) and treated with different percentages of bismuth followed by MTT assay for 24, 48, and 72 h. The red horizontal dotted line represents the basal group as 100%, and a blue continuous line represents the threshold for a cytotoxicity level of 75% of cell viability compared to the basal group (negative control). Each bar represents mean ± SD calculated from three independent experiments. ANOVA p < 0.01; post hoc Tukey test p < 0.05, lowercase letters are used to compare means; means sharing a superscript letter are not significantly different, n = 3

Fig. 3.

Effect of pH on the antibacterial activity and cell viability at 24 h. Black line: Cell viability, L929 cells. Red line: Antibacterial activity on S. mutans. Blue line: Antibacterial activity on P. gingivalis
Effect of pH on the antibacterial activity and cell viability at 24 h. Black line: Cell viability, L929 cells. Red line: Antibacterial activity on S. mutans. Blue line: Antibacterial activity on P. gingivalis

Fig. 4.

Micrographs of bismuth trioxide and the unhydrated cement sample. A. Bismuth trioxide; B, Bi15; C, Bi20; and D-Bi25. Yellow bar = 50μm, 350 × magnification
Micrographs of bismuth trioxide and the unhydrated cement sample. A. Bismuth trioxide; B, Bi15; C, Bi20; and D-Bi25. Yellow bar = 50μm, 350 × magnification

Fig. 5.

Surface micrographs of the hydrated cement sample. The long white particles corresponding to bismuth trioxide are not well incorporated into the cement. Left columns (A, C, E) are micrographs with secondary electrons. The right columns (B, D, F) are backscattering electron images. A–B: Bi15, C–D: Bi20, and E–F: Bi25. Yellow scale bar = 100 μm, 200 × magnification
Surface micrographs of the hydrated cement sample. The long white particles corresponding to bismuth trioxide are not well incorporated into the cement. Left columns (A, C, E) are micrographs with secondary electrons. The right columns (B, D, F) are backscattering electron images. A–B: Bi15, C–D: Bi20, and E–F: Bi25. Yellow scale bar = 100 μm, 200 × magnification

Means and standard deviation (in parenthesis) of solubility, setting time, and film thickness of Bi15, Bi20, and Bi25

Solubility (%) Setting time (min) Film thickness (mm)
Bi15 -2.70 (0.41)a 5:19 (1:41)a 0.01238 (0.00027)a
Bi20 0.24 (0.81)b 4:59 (1:26)a 0.01229 (0.00023)a
Bi25 -0.27 (0.82)b 4:41 (1:31)a 0.01230 (0.00043)a
ISO 6876 requirements < 3% No more than 110% of the manufacturer’s specifications. < 0.05 mm

pH means of the Bi15, Bi20, and Bi25 cements

1 h 3 h 24 h 48 h 72 h 7 d
Bi15 10.28 (0.25)a 10.68 (0.15)a 11.16 (0.21)a 10.94 (0.48)a 10.78 (0.70)a 9.98 (1.08) a
Bi20 10.49 (0.12)a 10.87 (0.10)a 11.32 (0.16)a 11.33 (0.22)a,b 11.07 (0.53)a 10.11 (0.92) a
Bi25 10.49 (0.13)a 10.88 (0.06)a 11.37 (0.12)a 11.47 (0.18)b 11.35 (0.35)a 10.96 (0.88) a
p 0.309 0.061 0.119 0.043 0.237 0.181

Reference strains used to determine the antimicrobial activity

Bacterial strain ATCC Gram/Respiration Associated with
Porphyromonas gingivalis 33277 −/anaerobic Periodontitis
Streptococcus mutans 25175 +/facultative anaerobic Dental caries
Idioma:
Inglés
Calendario de la edición:
4 veces al año
Temas de la revista:
Ciencia de los materiales, Ciencia de los materiales, otros, Nanomateriales, Materiales funcionales e inteligentes, Características y propiedades de los materiales
RSS Feed de revista