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Journal of Veterinary Research
Volumen 68 (2024): Edición 1 (March 2024)
Acceso abierto
Matrine and icariin can inhibit bovine viral diarrhoea virus replication by promoting type I interferon response
in vitro
Dongjie Cai
Dongjie Cai
,
Zifan Shen
Zifan Shen
,
Bin Tian
Bin Tian
,
Jie Chen
Jie Chen
,
Yilin Zhang
Yilin Zhang
,
Liuhong Shen
Liuhong Shen
,
Ya Wang
Ya Wang
,
Xiaoping Ma
Xiaoping Ma
y
Zhicai Zuo
Zhicai Zuo
| 23 mar 2024
Journal of Veterinary Research
Volumen 68 (2024): Edición 1 (March 2024)
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Publicado en línea:
23 mar 2024
Páginas:
35 - 44
Recibido:
19 ago 2023
Aceptado:
01 mar 2024
DOI:
https://doi.org/10.2478/jvetres-2024-0013
Palabras clave
bovine viral diarrhoea virus
,
matrine
,
icariin
,
antiviral effect
,
immunoregulation
© 2024 Dongjie Cai et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Fig. 1.
Cytotoxic effects of matrine and icariin on Madin–Darby bovine kidney (MDBK) cells. A – Matrine’s effect on MDBK cell viability; B – Half-maximal inhibitory concentration (IC50) of matrine on MDBK cells; C – Icariin’s effect on MDBK cell viability; D – IC50 of icariin on MDBK cells. Data are presented as mean ± standard deviation of three independent experiments. DMSO – dimethyl sulphoxide solvent control; Control – cell maintenance solution control group; **** – P-value < 0.0001
Fig. 2.
Effects of the matrine and icariin against bovine viral diarrhoea (BVDV) infection in Madin–Darby bovine kidney (MDBK) cells. A – anti-BVDV effect of matrine in MDBK cells; B – Half-maximal effective antiviral concentration (EC50) of matrine on MDBK cells; C – anti-BVDV effect of icariin in MDBK cells; D – EC50 of icariin on MDBK cells; * – P-value < 0.05; ** – P-value < 0.01; *** – P-value < 0.001; **** – P-value < 0.0001
Fig. 3.
Evaluation of different administration methods of matrine and icariin on bovine viral diarrhoea virus (BVDV) infection in Madin–Darby bovine kidney (MDBK) cells. A – Effects of different administration methods of matrine on the BVDV 5′ untranslated region (UTR); B – Effects of different administration methods of icariin on the BVDV 5′UTR; C – Evaluation of prophylactic effects of matrine and icariin on the BVDV 5′UTR at different time points; **** – P-value < 0.0001; BVDV group – BVDV and MDBK; Virucide group – BVDV and extract incubated at 4°C for 1 h, MDBK added subsequently; Co-treatment group – BVDV, extract and MDBK incubated at 4°C for 1.5 h; Pre-treatment group – MDBK and extract incubated at 37°C for 3 h, challenged with BVDV subsequently; Post-treatment group – MDBK and BVDV incubated at 37°C for 1 h, extract added subsequently
Fig. 4.
Effects of matrine and icariin on the expression levels of interferon alpha and beta (IFN-α/β) in Madin–Darby bovine kidney (MDBK) cells infected with bovine viral diarrhoea virus (BVDV). A – Effects of matrine and icariin on the secretion levels of IFN-β in MDBK cells; B – Effects of matrine on messenger RNA (mRNA) expression of IFN-α in MDBK cells; C – Effects of matrine on mRNA expression of IFN-β in MDBK cells; D – Effects of icariin on mRNA expression of IFN-α in MDBK cells; E – Effects of icariin on mRNA expression of IFN-β in MDBK cells; Control – negative group (without BVDV and drug); Matrine – group treated with matrine herb extract at 128 ng/mL; Icariin – group treated with icariin herb extract at 8 nM; + BVDV – group administered an extract and BVDV (100 TCID50); * – P-value < 0.05; ** – P-value < 0.01; *** – P-value < 0.001; **** – P-value < 0.0001
Fig. 5.
Effects of matrine and icariin on the messenger RNA (mRNA) expression levels of innate immune response genes in MDBK cells at different time points. A – Effects of matrine on the expression levels of retinoic acid–inducible gene I (RIG-I); B – Effects of matrine on the expression levels of toll-like receptor 3 (TLR3); C – Effects of matrine on the expression levels of interferon regulatory factor 3 (IRF3); D – Effects of icariin on the expression levels of RIG-I; E – Effects of icariin on the expression levels of TLR3; F – Effects of icariin on the expression levels of IRF3; * – P-value < 0.05; ** – P-value < 0.01; *** – P-value < 0.001; **** – P-value < 0.0001
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