Comparison of microscopy, card agglutination test for Trypanosoma evansi , and real-time PCR in the diagnosis of trypanosomosis in dromedary camels of the Abu Dhabi Emirate, UAE
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25 mar 2022
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Publicado en línea: 25 mar 2022
Páginas: 125 - 129
Recibido: 01 jul 2021
Aceptado: 03 ene 2022
DOI: https://doi.org/10.2478/jvetres-2022-0002
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© 2022 S. Habeeba et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Prevalence of infection, sensitivity, positive predictive value (PPV), negative predictive value (NPV) and Cohen’s kappa index (Kappa) (real-time PCR was taken as the reference standard)
| Method | Overall prevalencea | Sensitivityb | PPVb | NPVb | Kappac |
|---|---|---|---|---|---|
| Microscopy | 14/77 (18%) | 30.4 (17.1–43.7) | 100 (100) | 49.2 (38.0–60.4) | 0.26 (0.16–0.36) |
| CATT | 17/77 (22%) | 37.0 (23.0–50.9) | 100 (100) | 51.6 (40.5–62.8) | 0.09 (0.02–0.15) |
| Real-time PCR | 46/77 (60%) |
Comparison of microscopy and real-time PCR using McNemar’s chi-squared test
| Real-time PCR | Microscopy |
Total | |
|---|---|---|---|
| Positive | Negative | ||
| Positive | 14 | 32 | 46 |
| Negative | 0 | 31 | 31 |
| Total | 14 | 63 | 77 |
Comparison of CATT and real-time PCR using McNemar’s chi-squared test
| Real-time PCR | CATT |
Total | |
|---|---|---|---|
| Positive | Negative | ||
| Positive | 12 | 34 | 46 |
| Negative | 5 | 26 | 31 |
| Total | 17 | 60 | 77 |