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Correlation of the DNA concentration of human samples to electrical bioimpedance measurements: a pilot study

César A. Hernández-Salinas

César A. Hernández-Salinas

Centro Militar de Ciencias de la Salud – Escuela Militar de Graduados de SanidadUDEFA, CDMX, México
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Hernández-Salinas, César A.
, 
Alejandro Corzo-Cruz

Alejandro Corzo-Cruz

  • Centro Militar de Ciencias de la Salud – Escuela Militar de Graduados de SanidadUDEFA, CDMX, México
  • Instituto Politécnico Nacional – Escuela Superior de MedicinaCDMX, México
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Corzo-Cruz, Alejandro
, 
Virginia Sánchez-Monroy

Virginia Sánchez-Monroy

Instituto Politécnico Nacional – Escuela Superior de MedicinaCDMX, México
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Sánchez-Monroy, Virginia
, 
Jacobo E. Munguía-Cervantes

Jacobo E. Munguía-Cervantes

Instituto Politécnico Nacional – Centro de Nanociencias y Micro NanotecnologíasCDMX, México
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Munguía-Cervantes, Jacobo E.
 y 
César A. González-Díaz

César A. González-Díaz

Instituto Politécnico Nacional – Escuela Superior de MedicinaCDMX, México
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González-Díaz, César A.
  
14 ene 2023
Correlation of the DNA concentration of human samples to electrical bioimpedance measurements: a pilot study's Cover Image
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Publicado en línea: 14 ene 2023
Páginas: 132 - 135
Recibido: 21 nov 2022
DOI: https://doi.org/10.2478/joeb-2022-0018
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© 2022 César A. Hernández-Salinas, Alejandro Corzo-Cruz, Virginia Sánchez-Monroy, Jacobo E. Munguía-Cervantes, César A. González-Díaz, published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Fig.1

Interdigitated array microelectrodes consisting of a gold film embedded on a glass surface. EBiS measurements were performed on a 1 μL DNA drop.
Interdigitated array microelectrodes consisting of a gold film embedded on a glass surface. EBiS measurements were performed on a 1 μL DNA drop.

Fig.2

Electrophoresis assay for DNA extraction from twenty biological samples. The heterogeneity of fluorescence is evident in the lanes. Molecular weights in the witness lane (left side) are in Kpb.
Electrophoresis assay for DNA extraction from twenty biological samples. The heterogeneity of fluorescence is evident in the lanes. Molecular weights in the witness lane (left side) are in Kpb.

Fig.3

The EBiS of the DNA samples are portrayed in magnitude and phase. The values are expressed as the mean and standard error of measurements taken in triplicate. The spectra for the reference and miliQ water, included in the graph, were used as clean vehicles for DNA absorbance and EBiS measurements, respectively.
The EBiS of the DNA samples are portrayed in magnitude and phase. The values are expressed as the mean and standard error of measurements taken in triplicate. The spectra for the reference and miliQ water, included in the graph, were used as clean vehicles for DNA absorbance and EBiS measurements, respectively.

Fig.4

Dispersion diagrams of DNA concentration vs bioimpedance with a phase at 5.24 MHz. All DNA values (A) and DNA values below 100 ng/μL (B).
Dispersion diagrams of DNA concentration vs bioimpedance with a phase at 5.24 MHz. All DNA values (A) and DNA values below 100 ng/μL (B).

DNA was quantified by absorbance at 260 nm_ The mean value and the corresponding standard error is listed for each measurement (made in triplicate)_

Sample Mean (ng/μL) Std err
1 322.08 12.52
2 74.85 2.61
3 62.94 5.73
4 197.45 6.42
5 196.24 2.08
6 30.09 4.29
7 177.69 6.71
8 41.41 0.66
9 59.73 1.81
10 32.06 0.55
11 30.46 1.97
12 99.48 5.88
13 19.68 1.61
14 46.48 2.70
15 34.23 1.10
16 16.40 0.04
17 48.77 0.63
18 15.41 0.68
19 13.73 1.57
20 19.21 0.88
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Inglés
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Temas de la revista:
Ingeniería, Bioingeniería e ingeniería biomédica, Electrónica biomédica, Ciencias de la vida, Biofísica, Medicina, Ingeniería biomédica, Física, Espectroscopia y metrología
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