Morphological and Molecular note on the identity of Mylonchulus sigmaturus Cobb, 1917 (Nematoda: Mylonchulidae) from Pakistan

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Fig. 6.

Genetic pairwise distance estimating for different populations of M_ sigmaturus (numbers 1–7) and M_ arenicolus (number 8) [upper matrix indicates standard error)_

	species	Accession number	Locality	1	2	3	4	5	6	7	8
1	M. sigmaturus N18_988	OM278259	Pakistan		0.003	0.004	0.003	0.003	0.004	0.003	0.004
2	M. sigmaturus N19_988	OM278260	Pakistan	0.008		0.004	0.003	0.004	0.003	0.004	0.003
3	M. sigmaturus N21_988	OM278261	Pakistan	0.012	0.016		0.004	0.007	0.008	0.006	0.007
4	M. sigmaturus N22_988	OM278262	Pakistan	0.007	0.010	0.016		0.003	0.004	0.003	0.003
5	M. sigmaturus	AB361447	Japan	0.012	0.012	0.045	0.009		0.002	0.000	0.002
6	M. sigmaturus	AY284755	The Netherlands	0.014	0.010	0.047	0.012	0.008		0.002	0.001
7	M. sigmaturus	AB361446	Japan	0.012	0.012	0.033	0.009	0.000	0.008		0.002
8	M. arenicolus	AF036596	UK	0.013	0.010	0.042	0.010	0.007	0.002	0.008

Morphometric data of different populations of Mylonchulus sigmaturus along with other populations of M_ sigmaturus from different ecological zones_ All measurements are in µm except body length_

References	Present specimens		Koohkan et al., (2014)	Ahmad et al., (2010)	Farahmand et al., (2009)	De Bruin & Heyns (1992)	Chaves (1990)	Khan & Saeed (1987)	Coetzee (1966)	Mulvey (1961)
Locality	Pakistan		Iran	Japan	Iran	South Africa	Argentina	Pakistan	South Africa	Sweden
n	40♀♀	1♂	5 ♀♀	10♀♀	11♀♀	1 ♀	18 ♀♀	1♀	4 ♀♀	58♀♀
L	1.22 ±1.056 (1.04– 1.46)	1.21	1.294 ± 4.94 (1.22 – 1.36)	1.30 ± 0.08 (1.16 – 1.46)	1.20 ±1.29 (1.01 – 1.46)	1.25	1.12 ± 0.1 (1.0 – 1.32)	0.95	1.1 (1.1 – 1.2)	1 – 1.7
a	28.04 ±2.02 (25.8 – 33.7)	31.8	31.7 ± 1.3 (30.1 – 33.3)	29.7 ± 1.27 (27.3 – 31.8)	28.0 ± 2.1 (24.1 – 30.7)	34.7	29.7 ± 2.3 (26 – 36.2)	23	23.1 (29 – 33)	24 – 47
b	3.475 ± 0.196 (3.1 – 3.9)	3.4	3.5 ± 0.1 (3.4 – 3.6)	3.37 ± 0.12 (3.16 – 3.55)	3.7 ± 0.2 (3.2 – 3.9)	3.3	3.3 ± 0.2 (3.2 – 3.8)	4	3.2 (3.1 – 3.3)	3 – 4
c	35.17 ± 3.52 (26.0 – 40.93)	28.8	32.8 ± 5.6 (27.8 – 42.1)	35 ± 2.4 (30.5 – 38.6)	27.5 2.6 (26.6 – 30.7)	37.9	30 ± 2.6 (2558 – 32.2)	17.9	26.9 (25 – 29)	24 – 39.2
ć	1.205 ± 0.115 (1.0 – 1.42)	1.2	1.5 ± 0.2 (1.2 – 1.9)	1.21 ± 0.07 (1.1 – 1.3)	1.4 ± 0.2 (1.1 – 1.8)	1.5	1.4 ± 0.2 (1.2 – 1.6)	1.2	1.4	1 – 1.8
V	65.72 ± 2.217 (60.41 – 70.6)	--	64 ± 1.1 (63.1 – 65.9)	65.14 ± 1.4 (62.9 – 67.3)	62.8± 1.9 (59.7 – 66.4)	65	64.6 ± 1.5 (62 – 68)	58.4	66.0 (66 – 67)	50 – 77
G1	9.92 ± 1.50 (6.1 – 14.0)	--	18.7 ± 1.5 (18 – 20)	14.15 ± 3.69 (7 – 20)	12.1 ± 0.8 (11.1 – 12.9)	-	-	13.7	1.4 (9.2 – 11.8)	-
G2	9.731 ± 1.77 (5.4 – 14.4)	--	18.0 ± 4.0 (13 – 22)	11.3 ± 2.5 (9 – 18)	10.5± 1.3 (8.5 – 11.7)	-	-	-	9.5 (9.1 – 9.8)	-
Lip region width	24.54 ± 1.44 (22 – 27)	22	24.2 ± 1.5 (23 – 27)	25.1 ± 1.4 (23 – 27)	24.6±1.8 (22.4 – 28)	23	21.5 ± 1.8 (20 – 25)	23.2	19.1	-
Buccal cavity length	25.75 ± 1.198 (25 – 27)	26	24.7 ± 0.8 (24 – 26)	25 ± 1.06 (24 – 27)	22.9± 1.4 (21 – 25.2)	26	22.7 ± 1.4 (20 – 25)	26.5	22.1	20 – 31
Buccal cavity width	16 ± 2.01 (15 – 17)	15	14.1 ± 0.4 (14 – 15)	15.2 ± 0.4 (15 – 16)	15.8±0.7 (15.4 – 16.8)	13	14.3 ± 1.0 (13 – 16)	13.1	9.6	11 – 17
Dorsal tooth apex % of buccal cavity	78.5 ± 1.945% (74 – 80%)	77	81.2 ± 2.6 (79 – 85)		80.2±4.6 (72.2 – 87.6)	15	77.2 ± 2.6 (74 – 81)		20	-
Amphid position from anterior end	9 ± 1.07 (8 – 10)	11	12±0.0 (12)	-	-	-	-	-		-
Amphidial aperture diameter	4.50±0.31 (4 – 5)	5	3.0±0.0 (3)	-	-	-	-	-	-	-
Excretory pore from anterior end	102.5 ± 3.05 (94 – 113)	--	-	-	-	-	-	-	-	-
Nerve ring from anterior end	82.166 ± 3.46 (80– 90)	84	104.9±11.3 (89 – 117)	108 ± 3.6 (102 – 114)	99.6±11.9 (87.5 – 122.5)	-	-		-	-
Neck length	348.8 ± 17.6 (318 – 372)	352	367.5± 10.2 (355 – 379)	386 ± 12 (370 – 410)	330.6±29.3 (297.5 – 392)	-	293.7 ± 12.3 (279 – 309)		-	-
Body diameter at:		--		-	-	-	-	-	-	-
Neck base	37.12 ± 3.91 (30 – 47)	36	37.1±1.8 (35 – 39)	-	-	-	-	-	-	-
Mid body	43.75 ± 3.78 (38 – 50)	38	40.9±2.3 (39 – 44)	-	-	-	-	-	-	-
Anus	29.04 ± 1.92 (26 – 33)	37	27.3±3.0 (24 – 31)	-	30.9±1.4 (28 – 31.5)	-	-	-	-	-
Rectum	21.91 ± 1.49 (20 – 24)	--	21.4±2.1 (18 – 24)	21 ± 2.4 (17 – 25)	24.5±3.1 (21 – 28)	-	-		-	-
Tail length	35 ± 4.20 (30 – 50)	42	40.3±6.2 (31 – 47)	38.6 ± 2.6 (35 – 42)	44.2±5.5 (35 – 52.5)	33	33.2 ± 3.5 (29 – 37)		26.5	-
Tail length as % of total body length	2.76 ± 0.288 (2.26% – 3.38%)	3.46	3.1±0.5 (2 – 4)	-	-	-	-	-	-	-
Spicules	--	46	--	--	--	--	--	--	--	--
Gubernaculum	--	18	--	--	--	--	--	--	--	--

Factor score for different populations of M_ sigmaturus_

Observation	F1	F2
P1	−0.629	−1.704
P2	−0.112	−2.305
P3	0.224	0.378
P4	0.436	0.645
Koohkan et al., (2014); Iran	−0.725	1.769
Chaves (1990); Argentina	−2.506	0.567
Coetzee (1966); South Africa	−2.479	−0.303
de Bruin & Heyns (1992); South Africa	−1.260	2.193
Khan & Saeed (1987); Pakistan	−1.636	−4.145
Ahmad et al., (2010); Japan	−0.034	1.920
Farahmand et al., (2009); Iran	0.122	−0.773
Mulvey (1961); Sweden	−0.414	2.491
M. arenicolus	9.168	−0.271

Factor loading for different populations of M_ sigmaturus_

	F1	F2
Body length (BL)	0.486	0.360
a	0.170	0.833
b	0.110	−0.568
c	0.106	0.550
c′	−0.621	0.398
V	0.116	0.430
G1	−0.799	0.256
G2	−0.816	0.231
Lip region width (LW)	0.897	−0.109
Buccal cavity length (BCL)	0.576	0.164
Buccal cavity width (BCW)	0.614	0.350
Dorsal tooth apex (DTA%)	0.141	−0.357
Amphid to anterior end	0.109	0.532
Amphid diameter	0.082	−0.769
Nerve ring to anterior end (NR)	0.412	0.150
Neck length	0.851	0.005
Neck base diameter (NBD)	0.104	0.646
Mid-body diameter (MBD)	0.980	−0.123
Anal body diameter (ABD)	0.867	−0.180
Rectum	0.941	−0.123
Tail length (TL)	0.903	0.083
TL%BL	0.803	0.380