Treatment | Concentration (mg · L -1) | No. of explants | Multiplication factor | |
---|---|---|---|---|
6-BA | NAA | |||
C1 | 0.5 | 0.1 | 60 | 1.79 ± 0.15 g |
C2 | 0.5 | 0.5 | 60 | 1.70 ± 0.12 g |
C3 | 0.5 | 1.0 | 60 | 2.28 ± 0.15 f |
C4 | 1.0 | 0.1 | 60 | 3.21 ± 0.28 d |
C5 | 1.0 | 0.5 | 60 | 2.89 ± 0.17 e |
C6 | 1.0 | 1.0 | 60 | 2.36 ± 0.18 f |
C7 | 1.5 | 0.1 | 60 | 4.56 ± 0.25 b |
C8 | 1.5 | 0.5 | 60 | 5.24 ± 0.32 a |
C9 | 1.5 | 1.0 | 60 | 3.77 ± 0.19 c |
Genotype | The time at which a secretion began to appear on the stigma (days after anthesis) | The time at which a secretion began to peak (days after anthesis) | The time at which the stigma began to be receptive (days after anthesis) | The time at which stigma receptivity began to peak (days after anthesis) |
---|---|---|---|---|
‘3-1’ | 1 | 2 | 2 | 3 |
‘5-4’ | 2 | 4 | - | - |
‘5-35’ | 1 | 3 | 2 | 4 |
‘0-0.2’ | 3 | 4 | 3 | 4 |
‘5-21’ | 2 | 3 | 3 | 5 |
‘3-12’ | 0 | 2 | 3 | 4 |
Treatment group | NAA (mg · L-1) | 6-BA (mg · L-1) |
---|---|---|
1 | 0.5 | 0.1 |
2 | 0.5 | 0.5 |
3 | 0.5 | 1.0 |
4 | 1.0 | 0.1 |
5 | 1.0 | 0.5 |
6 | 1.0 | 1.0 |
7 | 1.5 | 0.1 |
8 | 1.5 | 0.5 |
9 | 1.5 | 1.0 |
Number | Varieties | Germination rate (%) | Average germination rate (%) | ||
---|---|---|---|---|---|
A7 | Pollyanna | 88.34 | 86.08 | 90.22 | 88.21 |
A8 | Prato | 94.87 | 92.67 | 93.78 | 93.77 |
A9 | Sorbonne | 80.53 | 83.72 | 86.47 | 83.57 |
Cultivar | Female parent | Male parent | Plant location | Sampling time | Image |
---|---|---|---|---|---|
‘3-1’ (A1) | Pink Beauty | Pollyanna | Harbin | July 2017 | |
‘5-4’ (A2) | Pink Beauty | Pollyanna | Harbin | August 2017 | |
‘5-35’ (A3) | Pink Beauty | Pollyanna | Harbin | August, 2017 | |
‘0-0.2’ (A4) | Pink Beauty | Pollyanna | Harbin | September 2017 | |
‘5-21’ (A5) | Pollyanna | Prato | Harbin | September 2017 | |
‘3-1’ (A1) | Pollyanna | Sorbonne | Harbin | July 2017 |
Number | Cultivar | Receptivity | Seeding | Picture |
---|---|---|---|---|
1 | ‘3-1’ | ++ | ++ | |
2 | ‘5-4’ | − | − | − |
3 | ‘5-35’ | + | + | |
4 | ‘0-0.2’ | ++ | ++ | |
5 | ‘5-21’ | + | + | |
6 | ‘3-12’ | ++ | ++ |
Treatment | Concentration (mg · L -1) | No. of explants | Multiplication factor | |
---|---|---|---|---|
6-BA | NAA | |||
C1 | 0.5 | 0.1 | 60 | 1.63 ± 0.12 h |
C2 | 0.5 | 0.5 | 60 | 1.69 ± 0.15 h |
C3 | 0.5 | 1.0 | 60 | 2.01 ± 0.09 g |
C4 | 1.0 | 0.1 | 60 | 3.16 ± 0.24 e |
C5 | 1.0 | 0.5 | 60 | 3.48 ± 0.15 d |
C6 | 1.0 | 1.0 | 60 | 2.84 ±0.17 f |
C7 | 1.5 | 0.1 | 60 | 3.96 ± 0.29 c |
C8 | 1.5 | 0.5 | 60 | 4..96 ± 0.36 a |
C9 | 1.5 | 1.0 | 60 | 4.41 ± 0.33 b |
Combination | Seeding rate (%) | Seed morphology | 1,000 seed weight (g) |
---|---|---|---|
A1 × A7 | 32 | Orange, shrivelled | 0.017 |
A2 × A7 | 36 | Yellow, shrivelled | 0.021 |
A3 × A7 | 26 | Yellow, shrivelled | 0.018 |
A4 × A7 | 39 | Bright yellow, shrivelled | 0.016 |
A5 × A7 | 22 | Bright yellow, shrivelled | 0.019 |
A6 × A7 | 25 | Bright yellow, shrivelled | 0.017 |
Treatment group | 6-BA (mg · L-1) | NAA (mg · L-1) |
---|---|---|
1 | 0.5 | 0.1 |
2 | 0.5 | 0.5 |
3 | 0.5 | 1.0 |
4 | 1.0 | 0.1 |
5 | 1.0 | 0.5 |
6 | 1.0 | 1.0 |
7 | 1.5 | 0.1 |
8 | 1.5 | 0.5 |
9 | 1.5 | 1.0 |
Treatment | Concentration (mg · L -1) | No. of explants | Induction rate (%) | ||
---|---|---|---|---|---|
NAA | 6-BA | 5-4 | 5-35 | ||
CK | 0 | 0 | 60 | 7.33 ±0.51 h | 6.74 ± 0.57 h |
B1 | 0.5 | 0.1 | 60 | 12.69 ± 1.45 g | 13.41 ± 1.68 g |
B2 | 0.5 | 0.5 | 60 | 20.67 ± 1.87 f | 25.41 ± 1.03 f |
B3 | 0.5 | 1.0 | 60 | 28.49 ± 2.11 e | 33.84 ± 1.54 e |
B4 | 1.0 | 0.1 | 60 | 37.94 ± 2.16 d | 37.13 ± 2.37 e |
B5 | 1.0 | 0.5 | 60 | 70.98 ± 3.85 a | 46.77 ± 2.52 d |
B6 | 1.0 | 1.0 | 60 | 55.25 ± 1.96 c | 54.93 ± 2.26 c |
B7 | 1.5 | 0.1 | 60 | 59.66 ± 2.17 b | 66.58 ± 2.72 b |
B8 | 1.5 | 0.5 | 60 | 62.41 ± 3.03 b | 72.36 ± 3.04 a |
B9 | 1.5 | 1.0 | 60 | 61.56 ± 2.56 b | 65.29 ± 2.97 b |
Genotype | Stigma receptivity at different days after anthesis | |||||||
---|---|---|---|---|---|---|---|---|
0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | |
‘3-1’ | − | + | ++ | +++ | ++ | ++ | + | − |
‘5-4’ | − | − | − | − | − | − | − | − |
‘5-35’ | − | + | ++ | ++ | + | + | + | − |
‘0-0.2’ | − | + | + | ++ | + | + | + | − |
‘5-21’ | − | + | ++ | +++ | +++ | + | + | − |
‘3-12’ | − | + | +++ | +++ | ++ | ++ | + | − |
Cultivar | Series | Ploidy | Plant location | Sampling time | Image |
---|---|---|---|---|---|
Pollyanna (A7) | AA | Diploid | Harbin | July 2013 | |
Prato | AA | Diploid | Harbin | July 2013 | |
Sorbonne | OO | Diploid | Harbin | August 2013 | |
Pink Beauty | AA | Diploid | Harbin | August 2013 |