<abstract xmlns="http://www.w3.org/1999/xhtml">

<p><italic>Moringa oleifera</italic> seed and leaf are used traditionally for the treatment of various health problems (among others, hypertension, scrapes, skin infection, diabetes, genitourinary illnesses), and to boost the immune system, as well as to act as a contraceptive. In this study, the antibacterial activity of seed and leaf <italic>M. oleifera</italic> extracts on three-gram negative bacteria was investigated, and phytochemical analysis for the association of antibacterial activity with the active constituents in the plant was determined. Moreover, understanding of the mechanism of action was achieved by applying the Auto Dock Vina technique. The phytochemical screening of <italic>M. oleifera</italic> seed and leaf extracts exhibited the presence of alkaloids, carbohydrates, cardioactive glycosides, flavonoids, tannins, phenols, steroids and terpenoids. In silico results revealed that compounds (4-O-caffeoyl quinic acid, 4-(α-L-rhamnopyranosyloxyl)-benzylisothiocyanate); (Isoquercitrin, 4-(α-L-rhamnopyranosyloxy) benzyl glucosinolate); and (Astragalin, 4-(α-L-rhamnopyranosyloxy) benzyl glucosinolate) from leaf and seed have the highest binding affinity and very good interactions with Transcriptional Activator Protein (LasR), <italic>Klebsiella pneumoniae</italic> carbapenemase (KPC), and Malonyl-CoA-acyl carrier protein transacylase (FabD), respectively.</p>
</abstract>

Moringa oleifera seed and leaf are used traditionally for the treatment of various health problems (among others, hypertension, scrapes, skin infection, diabetes, genitourinary illnesses), and to boost the immune system, as well as to act as a contraceptive. In this study, the antibacterial activity of seed and leaf M. oleifera extracts on three-gram negative bacteria was investigated, and phytochemical analysis for the association of antibacterial activity with the active constituents in the plant was determined. Moreover, understanding of the mechanism of action was achieved by applying the Auto Dock Vina technique. The phytochemical screening of M. oleifera seed and leaf extracts exhibited the presence of alkaloids, carbohydrates, cardioactive glycosides, flavonoids, tannins, phenols, steroids and terpenoids. In silico results revealed that compounds (4-O-caffeoyl quinic acid, 4-(α-L-rhamnopyranosyloxyl)-benzylisothiocyanate); (Isoquercitrin, 4-(α-L-rhamnopyranosyloxy) benzyl glucosinolate); and (Astragalin, 4-(α-L-rhamnopyranosyloxy) benzyl glucosinolate) from leaf and seed have the highest binding affinity and very good interactions with Transcriptional Activator Protein (LasR), Klebsiella pneumoniae carbapenemase (KPC), and Malonyl-CoA-acyl carrier protein transacylase (FabD), respectively.

Phytochemical analysis and evaluation of antibacterial activity of <italic>Moringa oleifera</italic> extracts against gram-negative bacteria: an <italic>in vitro</italic> and molecular docking studies

Medical Analysis Department, Faculty of Applied Science

Current Issues in Pharmacy and Medical Sciences

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Phytochemical analysis and evaluation of antibacterial activity of  extracts against gram-negative bacteria: an  and molecular docking studies

Moringa oleifera seed and leaf are used traditionally for the treatment of various health problems (among others, hypertension, scrapes, skin infection,...

Phytochemical analysis and evaluation of antibacterial activity of Moringa oleifera extracts against gram-negative bacteria: an in vitro and molecular docking studies

Publicado en línea: 09 feb 2023

Páginas: 198 - 205

Recibido: 14 may 2022

Aceptado: 05 ago 2022

DOI: https://doi.org/10.2478/cipms-2022-0035

Palabras clave
AutoDock Vina, biological activity, molecular docking, multidrug-resistant

© 2022 Aryan R. Ganjo et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Phytochemical analysis and evaluation of antibacterial activity of Moringa oleifera extracts against gram-negative bacteria: an in vitro and molecular docking studies

Publicado en línea: 09 feb 2023

Páginas: 198 - 205

Recibido: 14 may 2022

Aceptado: 05 ago 2022

DOI: https://doi.org/10.2478/cipms-2022-0035

Palabras claveAutoDock Vina, biological activity, molecular docking, multidrug-resistant

© 2022 Aryan R. Ganjo et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
AutoDock Vina, biological activity, molecular docking, multidrug-resistant