<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The objective of this study was to investigate the effect of diets containing different levels of dried tuber of Jerusalem artichoke, <italic>Helianthus tuberosus</italic>, on skatole levels in backfat and on the <italic>CYP2E1</italic> mRNA expression in the liver of commercial crossbred pigs. A total of 23 uncastrated male pigs from 10 litters of a commercial crossbred population of Large White × (Landrace × Large White), were used in this study. Boars were randomly divided into four different dietary treatment groups – a control group (K1; 5 boars; without supplementation of Jerusalem artichoke) and three experimental groups (6 boars each) that were fed with the diet containing different levels of dried Jerusalem artichoke (K2 – 4.1%; K3 – 8.2%; K4 – 12.2%) for 14 days before slaughter. Significant effects of diet on skatole levels were observed between the control group and the experimental groups (P = 0.0078). The lowest level of skatole was in the K3 group with 8.2% of Jerusalem artichoke. As for <italic>CYP2E1</italic>, a negative correlation was observed between the levels of skatole and <italic>CYP2E1</italic> mRNA expression. Significant effect (P = 0.0055) was found in all experimental groups compared to the K1 group, and most pronounced in the K2 and K3 groups. The supplementation with Jerusalem artichoke resulted in lower level of skatole and higher <italic>CYP2E1</italic> mRNA expression. The results suggest that affecting the expression of <italic>CYP2E1</italic> by feed supplements could be an option to effectively reduce the levels of skatole in adipose tissue of entire male pigs.</p></abstract>

The objective of this study was to investigate the effect of diets containing different levels of dried tuber of Jerusalem artichoke, Helianthus tuberosus, on skatole levels in backfat and on the CYP2E1 mRNA expression in the liver of commercial crossbred pigs. A total of 23 uncastrated male pigs from 10 litters of a commercial crossbred population of Large White × (Landrace × Large White), were used in this study. Boars were randomly divided into four different dietary treatment groups – a control group (K1; 5 boars; without supplementation of Jerusalem artichoke) and three experimental groups (6 boars each) that were fed with the diet containing different levels of dried Jerusalem artichoke (K2 – 4.1%; K3 – 8.2%; K4 – 12.2%) for 14 days before slaughter. Significant effects of diet on skatole levels were observed between the control group and the experimental groups (P = 0.0078). The lowest level of skatole was in the K3 group with 8.2% of Jerusalem artichoke. As for CYP2E1, a negative correlation was observed between the levels of skatole and CYP2E1 mRNA expression. Significant effect (P = 0.0055) was found in all experimental groups compared to the K1 group, and most pronounced in the K2 and K3 groups. The supplementation with Jerusalem artichoke resulted in lower level of skatole and higher CYP2E1 mRNA expression. The results suggest that affecting the expression of CYP2E1 by feed supplements could be an option to effectively reduce the levels of skatole in adipose tissue of entire male pigs.

Effect of Dietary Supplementation with Dried Tuber of Jerusalem Artichoke on Skatole Level in Backfat and <italic>CYP2E1</italic> mRNA Expression in Liver of Boars

Department of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine

Annals of Animal Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

Effect of Dietary Supplementation with Dried Tuber of Jerusalem Artichoke on Skatole Level in Backfat and  mRNA Expression in Liver of Boars

The objective of this study was to investigate the effect of diets containing different levels of dried tuber of Jerusalem artichoke, Helianthus tuberosus, on...

Effect of Dietary Supplementation with Dried Tuber of Jerusalem Artichoke on Skatole Level in Backfat and CYP2E1 mRNA Expression in Liver of Boars

Publicado en línea: 28 oct 2021

Páginas: 1475 - 1489

Recibido: 11 may 2020

Aceptado: 25 nov 2020

DOI: https://doi.org/10.2478/aoas-2020-0119

Palabras clave
pig, boar taint, nutrition, RT -qPCR

© 2021 Kateřina Zadinová et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Effect of Dietary Supplementation with Dried Tuber of Jerusalem Artichoke on Skatole Level in Backfat and CYP2E1 mRNA Expression in Liver of Boars

Publicado en línea: 28 oct 2021

Páginas: 1475 - 1489

Recibido: 11 may 2020

Aceptado: 25 nov 2020

DOI: https://doi.org/10.2478/aoas-2020-0119

Palabras clavepig, boar taint, nutrition, RT -qPCR

© 2021 Kateřina Zadinová et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Palabras clave
pig, boar taint, nutrition, RT -qPCR