In vitro Production of Porcine Embryos: Current Status and Possibilities – A Review

This paper presents the current possibilities, state of knowledge and prospects of in vitro production (IVP) of pig embryos, which consists of in vitro oocyte maturation, in vitro fertilization and in vitro embryo culture. In pigs, oocyte maturation is one of the most important stages in the embryo IVP process. It determines the oocyte’s fertilization ability as well as its embryonic development. Through many research studies of the proper selection of oocytes and appropriate maturation medium composition (especially the addition of various supplements), the in vitro maturation of pig oocytes has been significantly improved. Recent studies have demonstrated that modifications of the diluents and in vitro fertilization media can reduce polyspermy. Furthermore, several adjustments of the porcine culture media with the addition of some supplements have enhanced the embryo quality and developmental competence. These updates show the progress of IVP in pigs that has been achieved; however, many problems remain unsolved.