At the present time, crop rotations are constantly adapted to economic conditions and one or two main crops are grown in agricultural farms. This has a negative impact on the environment. The optimization of grain yield and quality of winter wheat are dependent not only on the appropriate crop rotation but also on the nitrogen fertilisation. The aim of this study was to examine the effect of crop rotations, fertilisation and preceding crop on grain yield and quality of winter wheat during the years 2015/2016 – 2017/2018. The grain yield of winter wheat after preceding crop pea in crop rotation with 40% proportion of cereals was statistically higher (5.91 t/ha) than in crop rotation with 80% share of cereals (5.55 t/ha). In the treatment with mineral fertilisation and organic manure Veget® incorporation, the grain yield was statistically higher (6.00 t/ha) than in the treatment with mineral fertilisation (5.50 t/ha). According the standard STN 46 1100-2:2018 based on the wet gluten content, the winter wheat grain was classified into the class E by mineral fertilisation + organic manure Veget® in all monitored years. In 2017 the winter wheat grain was categorized into the class P based on wet gluten content in crop rotations with 80% proportion of cereals in the treatment with fertilisers only after preceding crop winter barley.