Acceso abierto

‘Cell cycle’ and ‘cell death’- related genes are differentially expressed during long – term in vitro real-time cultivation of porcine oviductal epithelial cells

Magdalena Kulus
Magdalena Kulus
, 
Jakub Kulus
Jakub Kulus
, 
Małgorzata Popis
Małgorzata Popis
, 
Blanka Borowiec
Blanka Borowiec
, 
Katarzyna Stefańska
Katarzyna Stefańska
, 
Piotr Celichowski
Piotr Celichowski
, 
Mariusz J. Nawrocki
Mariusz J. Nawrocki
, 
Klaus P. Brüssow
Klaus P. Brüssow
, 
Bartosz Kempisty
Bartosz Kempisty
, 
Michal Jeseta
Michal Jeseta
, 
Paweł Antosik
Paweł Antosik
 y 
Dorota Bukowska
Dorota Bukowska
   | 08 nov 2019
Medical Journal of Cell Biology's Cover Image
Acerca de este artículo
Artículo anterior
Artículo siguiente
Cite
Publicado en línea: 08 nov 2019
Páginas: 90 - 99
Recibido: 06 ago 2019
Aceptado: 20 sept 2019
DOI: https://doi.org/10.2478/acb-2019-0012
Palabras clave
© 2019 Magdalena Kulus, Jakub Kulus, Małgorzata Popis, Blanka Borowiec, Katarzyna Stefańska, Piotr Celichowski, Mariusz J. Nawrocki, Klaus P. Brüssow, Bartosz Kempisty, Michal Jeseta, Paweł Antosik, Dorota Bukowska, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Heat map representation of differentially expressed genes belonging to the to “cell cycle” and “cell death” GO BP terms. Arbitrary signal intensity acquired from microarray analysis is represented by colors (green, higher; red, lower expression). Log2 signal intensity values for any single gene were resized to Row Z-Score scale (from -2, the lowest expression to +2, the highest expression for single gene)
Heat map representation of differentially expressed genes belonging to the to “cell cycle” and “cell death” GO BP terms. Arbitrary signal intensity acquired from microarray analysis is represented by colors (green, higher; red, lower expression). Log2 signal intensity values for any single gene were resized to Row Z-Score scale (from -2, the lowest expression to +2, the highest expression for single gene)

Figure 2

The circle plot showing the differently expressed genes and z-score of “cell cycle” and “cell death” GO BP terms. The outer circle shows a scatter plot for each term of the fold change of the assigned genes. Red circles display upregulation and blue ones downregulation. The inner circle shows the z-score of each GO BP term. The width of the each bar corresponds to the number of genes within GO BP term and the color corresponds to the z-score
The circle plot showing the differently expressed genes and z-score of “cell cycle” and “cell death” GO BP terms. The outer circle shows a scatter plot for each term of the fold change of the assigned genes. Red circles display upregulation and blue ones downregulation. The inner circle shows the z-score of each GO BP term. The width of the each bar corresponds to the number of genes within GO BP term and the color corresponds to the z-score

Figure 3

The representation of the mutual relationship 10 most upregulated and 10 most downregulated genes that belongs to the to “cell cycle” and “cell death” GO BP terms. The ribbons indicate which gene belongs to which categories. The middle circle represents logarithm from fold change (LogFC) between D7/D1, D15/D1 and D30/D1 respectively. The genes were sorted by logFC from most to least changed gene
The representation of the mutual relationship 10 most upregulated and 10 most downregulated genes that belongs to the to “cell cycle” and “cell death” GO BP terms. The ribbons indicate which gene belongs to which categories. The middle circle represents logarithm from fold change (LogFC) between D7/D1, D15/D1 and D30/D1 respectively. The genes were sorted by logFC from most to least changed gene

Figure 4

Heatmap showing the gene occurrence between 10 most upregulated and 10 most downregulated genes that belongs “cell cycle” and “cell death” GO BP terms. The red color is associated with gene occurrence in the GO Term. The intensity of the color is corresponding to amount of GO BP terms that each gene belongs to
Heatmap showing the gene occurrence between 10 most upregulated and 10 most downregulated genes that belongs “cell cycle” and “cell death” GO BP terms. The red color is associated with gene occurrence in the GO Term. The intensity of the color is corresponding to amount of GO BP terms that each gene belongs to

Figure 5

STRING-generated interaction occurrence between 10 most upregulated and 10 most dowregulated genes that belongs to “cell cycle” and “cell death” GO BP terms. The intensity of the edges reflects the strength of interaction score
STRING-generated interaction occurrence between 10 most upregulated and 10 most dowregulated genes that belongs to “cell cycle” and “cell death” GO BP terms. The intensity of the edges reflects the strength of interaction score

Gene symbols, fold changes in expression, Entrez gene IDs and corrected p values of studied genes

GENE SYMBOLFOLD. CHANGE D15/D7FOLD. CHANGE D30/D7FOLD. CHANGE D30/D15ADJUSTED P.VALUE D15/D7ADJUSTED P.VALUE D30/D7ADJUSTED P.VALUE D30/D15GENEID
SERPINB20,0411990,0126770,0082394,60E-067,45E-072,31E-071,01E+08
CD2740,044240,044430,0418342,50E-067,45E-072,31E-07574058
TXNIP0,0821790,644530,635231,11E-050,0240190,017645733688
IL240,0833710,0621920,0713999,50E-063,21E-061,76E-061,01E+08
HSH2D0,1235750,0834640,0944092,25E-055,06E-063,79E-061,01E+08
IDO10,1345440,121110,1233317,22E-053,53E-052,36E-051,01E+08
MX10,1473130,3559750,4891621,87E-050,0001970,00096397128
IFIT30,1721010,1950120,313171,87E-051,28E-054,60E-051E+08
SNAI20,1837660,1345210,1300790,0004110,0001297,85E-05641345
XAF10,1961550,4563550,663620,0001620,0043190,06338---
PTTG15,5734074,8399270,8188915,27E-054,98E-050,269018397015
CDKN2B5,9060765,2792485,0317116,22E-063,39E-061,81E-06397227
KIF20A6,1828946,0011820,5881950,0003190,0002490,0638921,01E+08
KIF236,496867,1059657,1047520,0006470,0003820,0002711,01E+08
TTK6,5958336,0641420,6291740,0001940,0001630,078507---
DLGAP57,4038446,5515990,6997769,17E-058,22E-050,1310541,01E+08
GAS2L37,7391687,0359031,5594178,27E-056,71E-050,0647061E+08
UBE2C8,5232984,7179570,3726580,0009140,003660,0256481E+08
HHEX9,44744711,8239511,307392,69E-059,59E-066,25E-06397232
CENPF9,5062426,9604970,7732390,0004840,0007420,522241,01E+08
eISSN:
2544-3577
Idioma:
Inglés
Calendario de la edición:
4 veces al año
Temas de la revista:
Life Sciences, Molecular Biology, Biochemistry
RSS Feed de revista