Test | Allele 1 | Allele 2 |
---|---|---|
RBC-FluoGene D weak/variant | Possible |
|
wRHD BeadChip | Possible |
|
Nucleotide sequencing | ||
Allele recognized | Known | Novel |
Nucleotide sequence (GenBank) | KF712273 | MT980847 |
Amino acid change(s) | T201R, F223V, I342T | I204K |
Serologic weak D phenotype15 | DAR1.2 (weak D 4.2.2)18 | Weak D type 161 |
ISBT terminology16 |
Test | Result |
---|---|
ABO group | AB (mixed-field agglutination for A)* |
RhD phenotype | Serologic weak D phenotype† |
RhCE phenotype | C+E–c+e+ |
Antibody detection test | Negative |
Direct antiglobulin test | Negative |
D variant and serologic reaction strength* |
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Study sample |
Control samples |
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Monoclonal anti-D† |
Weak D type |
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Clone | Isotype | epD | Patient CDe/cDe | 19‡ CDe/cde | 1 CDe/cde | 4.0§ cDe/cde | 4.1 cDe/cde | 4.2.2¶ cDe/cde | 45 CDe/cde | 64 cDe/cde | 88 cDE/cde | D+ cDe/cde | D– cde/cde |
LHM76/58 | IgG1λ | 8.1 | 4+ | + | 4+ | 4+ | 4+ | 3+ to 4+ | 4+ | 4+ | 4+ | 4+ | 0 |
LHM76/59 | IgG1 | 15.1 | 4+ | + | 4+ | 4+ | 4+ | 3+ to 4+ | 4+ | 4+ | 4+ | 4+ | 0 |
LHM174/102 | IgG3κ | 1.2 | 2+ | + | 0 | 0 | 0 | 0 | 4+ | 2+ | 1+ | 4+ | 0 |
LHM50/2B | IgG1λ | 6.3 | 4+ | NT | 4+ | 4+ | 4+ | 3+ to 4+ | 4+ | 4+ | 4+ | 4+ | 0 |
LHM169/81 | IgG3κ | 1.1 | 4+ | + | 4+ | 4+ | 4+ | 2+ to 3+ | 4+ | 4+ | 4+ | 4+ | 0 |
ESD1 | IgG1κ | 4.1 | 4+ | NT | 4+ | 4+ | 4+ | 3+ to 4+ | 4+ | 4+ | 4+ | 4+ | 0 |
LHM76/55 | IgG1κ | 3.1 | 4+ | + | 4+ | 4+ | 4+ | 3+ to 4+ | 4+ | 4+ | 4+ | 4+ | 0 |
LHM77/64 | IgG1κ | 9.1 | 4+ | NT | 4+ | 4+ | 4+ | 3+ to 4+ | 4+ | 4+ | 4+ | 4+ | 0 |
LHM70/45 | IgG1λ | 1.2 | 3+ | + | 3+ | 3+ | 3+ | 0 to 2+ | 3+ | 3+ | 3+ | 4+ | 0 |
LHM59/19 | IgG3κ | 8.1 | 4+ | NT | 3+ | 3+ | 3+ | 0 to 2+ | 4+ | 3+ | 3+ | 4+ | 0 |
LHM169/80 | IgG3λ | 6.3 | 4+ | NT | 4+ | 4+ | 4+ | 3+ to 4+ | 4+ | 4+ | 4+ | 4+ | 0 |
LHM57/17 | IgG1λ | 6.3 | 0 | NT | 0 | 0 | 0 | 0 to 2+ | NT | 0 | 0 | 1+ | 0 |
LDM1 | IgM | 6.5 | 3+ | NT | 0 | 4+ | 3+ | 0 to 2+ | 4+ | 0 | 2+ | 4+ | 0 |
Samples | |||||||||
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Study sample |
Control samples |
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Weak D type |
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Test method* | Patient CDe/cDe | 4.0† cDe/cde | 4.1 cDe/cde | 4.2.2‡ cDe/cde | 45 CDe/cde | 64 cDe/cde | 88 cDE/cde | D+ cDe/cde | D– cde/cde |
Tube | 0 | 2+ | 1+ | 0 | 2+ | 0 | 0 | 3+ | 0 |
Gel matrix | 3+ | 3+ | 3+ | 1+ to 3+ | 4+ | 3+ | 3+ | 4+ | 0 |