<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> To identify novel pathways involved in the pathogenesis of ketosis, an isobaric tag for relative and absolute quantitation/mass spectrometry was used to define differences in protein expression profiles between healthy dairy cows and those with clinical or subclinical ketosis.<bold>Material and Methods:</bold> To define the novel pathways of ketosis in cattle, the differences in protein expression were analysed by bioinformatics. Go Ontology and Pathway analysis were carried out for enrich the role and pathway of the different expression proteins between healthy dairy cows and those with clinical or subclinical ketosis.<bold>Results:</bold> Differences were identified in 19 proteins, 16 of which were relatively up-regulated while the remaining 3 were relatively down-regulated. Sorbitol dehydrogenase (SORD) and glyceraldehyde-3-phosphate dehydrogenase (G3PD) were up-regulated in cattle with ketosis. SORD and G3PD promoted glycolysis. These mechanisms lead to pyruvic acid production increase and ketone body accumulation.<bold>Conclusion:</bold> The novel pathways of glycolysis provided new evidence for the research of ketosis.</abstract>

Introduction: To identify novel pathways involved in the pathogenesis of ketosis, an isobaric tag for relative and absolute quantitation/mass spectrometry was used to define differences in protein expression profiles between healthy dairy cows and those with clinical or subclinical ketosis.Material and Methods: To define the novel pathways of ketosis in cattle, the differences in protein expression were analysed by bioinformatics. Go Ontology and Pathway analysis were carried out for enrich the role and pathway of the different expression proteins between healthy dairy cows and those with clinical or subclinical ketosis.Results: Differences were identified in 19 proteins, 16 of which were relatively up-regulated while the remaining 3 were relatively down-regulated. Sorbitol dehydrogenase (SORD) and glyceraldehyde-3-phosphate dehydrogenase (G3PD) were up-regulated in cattle with ketosis. SORD and G3PD promoted glycolysis. These mechanisms lead to pyruvic acid production increase and ketone body accumulation.Conclusion: The novel pathways of glycolysis provided new evidence for the research of ketosis.

Identification of novel pathways in pathogenesis of ketosis in dairy cows <italic>via</italic> iTRAQ/MS

Department of College of Animal Science and Veterinary Medicine, Heilongjiang BaYi Agriculture University, Daqing 163319,

Department of Synergetic Innovation Center of Food Safety and Nutrition, Northeast Agricultural University, Harbin 150030,

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Identification of novel pathways in pathogenesis of ketosis in dairy cows  iTRAQ/MS

Introduction: To identify novel pathways involved in the pathogenesis of ketosis, an isobaric tag for relative and absolute quantitation/mass spectrometry was...

Identification of novel pathways in pathogenesis of ketosis in dairy cows via iTRAQ/MS

Publicado en línea: 23 sept 2016

Páginas: 309 - 314

Recibido: 08 oct 2015

Aceptado: 25 jul 2016

DOI: https://doi.org/10.1515/jvetres-2016-0047

Palabras clave
dairy cows, ketosis, pathogenesis, proteins, iTRAQ/MS

© 2016 Shi Shu et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Identification of novel pathways in pathogenesis of ketosis in dairy cows via iTRAQ/MS

Publicado en línea: 23 sept 2016

Páginas: 309 - 314

Recibido: 08 oct 2015

Aceptado: 25 jul 2016

DOI: https://doi.org/10.1515/jvetres-2016-0047

Palabras clavedairy cows, ketosis, pathogenesis, proteins, iTRAQ/MS

© 2016 Shi Shu et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
dairy cows, ketosis, pathogenesis, proteins, iTRAQ/MS