1. bookVolumen 60 (2016): Edición 3 (September 2016)
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eISSN
2450-8608
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30 Mar 2016
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access type Acceso abierto

Isolation and characterisation of peroxiredoxin 6 promoter from sheep (Ovis aries)

Publicado en línea: 23 Sep 2016
Volumen & Edición: Volumen 60 (2016) - Edición 3 (September 2016)
Páginas: 315 - 321
Recibido: 25 Mar 2016
Aceptado: 02 Sep 2016
Detalles de la revista
License
Formato
Revista
eISSN
2450-8608
Primera edición
30 Mar 2016
Calendario de la edición
4 veces al año
Idiomas
Inglés
Abstract

Introduction: Peroxiredoxin 6 (Prdx6) is a bifunctional protein and a unique 1-Cys Prdx of the peroxiredoxin family. The expression and regulation of Prdx6 are implicated in numerous physiological and pathological processes.

Material and Methods: Eight stepwise truncated DNA fragments obtained from the 5′-flank region of the Prdx6 gene were prepared and subcloned into the pSEAP2-Enhancer vectors. To investigate the transcriptional activity of the truncated DNA fragments, the recombinant plasmids were transfected into the COS-1 cells and the transcriptional activity was measured via assaying the expression of the reporter gene of the secreted alkaline phosphatase.

Results: A 3.4 kb 5′-upstream flank region of the Prdx6 gene was cloned and sequenced. The region from −108 nt to −36 nt of the 5′-flanking region of the Prdx6 gene contained basal transcriptional activity.

Conclusion: This result provides the basis for further studies on the gene regulation of the Prdx6-mediated biological processes and on screening for the transacting factors that interact with cis-acting elements of the Prdx6 gene promoter.

Keywords

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