<abstract xmlns="http://www.w3.org/1999/xhtml"><p>This study aimed to investigate the prevalence of paramphistomosis and confirm the species identity of rumen flukes from sheep and cattle of Sardinia (Italy), by molecular methods. From 2011 to 2014, 381 sheep and 59 cattle farms were selected and individual faecal samples were run on 15 sheep and 5 cattle for each farm, respectively. The prevalence at the slaughterhouse was calculated by examination of 356 sheep and 505 cattle. 13adult flukes collected from sheep and cattle and 5 belonging to the historical collection of Laboratory of Parasitology at the Department of Veterinary Medicine of Sassari, previously classified as <italic>Paramphistomum</italic> spp., were used for PCR amplification and sequencing of the ITS2+ rDNA. Previously classified <italic>Paramphistomum</italic> leydeni from South America were used as controls.</p><p>The EPG prevalence was 13.9 % and 55.9 % for sheep and cattle farms respectively. At slaughterhouses, paramphistomes were found in 2 % of the sheep and 10.9 % of the examined cows. Conversely to the latest reports, the sequences comparison showed that all the Sardinian rumen flukes belong to <italic>Calicophoron daubneyi</italic>.</p></abstract>

This study aimed to investigate the prevalence of paramphistomosis and confirm the species identity of rumen flukes from sheep and cattle of Sardinia (Italy), by molecular methods. From 2011 to 2014, 381 sheep and 59 cattle farms were selected and individual faecal samples were run on 15 sheep and 5 cattle for each farm, respectively. The prevalence at the slaughterhouse was calculated by examination of 356 sheep and 505 cattle. 13adult flukes collected from sheep and cattle and 5 belonging to the historical collection of Laboratory of Parasitology at the Department of Veterinary Medicine of Sassari, previously classified as Paramphistomum spp., were used for PCR amplification and sequencing of the ITS2+ rDNA. Previously classified Paramphistomum leydeni from South America were used as controls.The EPG prevalence was 13.9 % and 55.9 % for sheep and cattle farms respectively. At slaughterhouses, paramphistomes were found in 2 % of the sheep and 10.9 % of the examined cows. Conversely to the latest reports, the sequences comparison showed that all the Sardinian rumen flukes belong to Calicophoron daubneyi.

<italic>Calicophoron daubneyi</italic> in sheep and cattle of Sardinia, Italy

Laboratory of Parasitology, Veterinary Teaching Hospital, Veterinary Department, University of Sassari, Italy

Veterinary Practitioner, Martini Zootecnica, Italy

Veterinary Faculty, National University of La Plata, Buenos Aires, Argentine. National Scientific and Technical Research Council (CONICET), Argentine

Inter-University Center for Research in Parasitology (CIRPAR), Via della Veterinaria 1 – 80137, Naples, Italy

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

This study aimed to investigate the prevalence of paramphistomosis and confirm the species identity of rumen flukes from sheep and cattle of Sardinia (Italy),...

Calicophoron daubneyi in sheep and cattle of Sardinia, Italy

Article Category: Research Note

Publicado en línea: 10 mar 2016

Páginas: 87 - 93

Recibido: 22 abr 2015

Aceptado: 13 jul 2015

DOI: https://doi.org/10.1515/helmin-2015-0069

Palabras clave
rumen fluke, paramphistomosis, sheep, cattle

© 2016 Institute of Parasitology, SAS, Košice

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Calicophoron daubneyi in sheep and cattle of Sardinia, Italy

Article Category: Research Note

Publicado en línea: 10 mar 2016

Páginas: 87 - 93

Recibido: 22 abr 2015

Aceptado: 13 jul 2015

DOI: https://doi.org/10.1515/helmin-2015-0069

Palabras claverumen fluke, paramphistomosis, sheep, cattle

© 2016 Institute of Parasitology, SAS, Košice

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Palabras clave
rumen fluke, paramphistomosis, sheep, cattle