<abstract xmlns="http://www.w3.org/1999/xhtml">The paramyosin (Pmy) protein has been presented as a potential vaccine candidate against <italic>Schistosoma</italic> spp. However, it remains elusive whether it works in controlling cystic echinococcosis (CE), which is caused by the larval stages of <italic>Echinococcus granulosus</italic> (<italic>E. granulosus</italic>). This study investigated the characteristics of <italic>E. granulosus</italic> Pmy (EgPmy) using <italic>in silico</italic> analysis and evaluated its potential as an epitope vaccine. The secondary structure was predicted by SOPMA software and linear B-cell epitopes were screened by the Kolaskar and Tongaonkar’s method on IEBD while conformational B-cell epitopes were predicted by the Ellipro. Additionally, the epitopes of cytotoxic T lymphocyte (CTL) were analyzed by the NetCTL-1.2 server. The results showed that α-helices, extended strands, random coils and β-turns accounted for 84.82 %, 6.60 %, 5.56 % and 3.01 % in EgPmy’s secondary structure, respectively. A total of 29 linear B-cell epitopes and 6 conformational epitopes were identified together with 25 CTL epitopes. The CTL epitope 709KLEEAEAFA717 showed a high potential to elicit CTL response. These results suggested that EgPmy has a strong immunogenicity, which could serve as a reference for the development of EgPmy-based epitope vaccine against CE.</abstract>

The paramyosin (Pmy) protein has been presented as a potential vaccine candidate against Schistosoma spp. However, it remains elusive whether it works in controlling cystic echinococcosis (CE), which is caused by the larval stages of Echinococcus granulosus (E. granulosus). This study investigated the characteristics of E. granulosus Pmy (EgPmy) using in silico analysis and evaluated its potential as an epitope vaccine. The secondary structure was predicted by SOPMA software and linear B-cell epitopes were screened by the Kolaskar and Tongaonkar’s method on IEBD while conformational B-cell epitopes were predicted by the Ellipro. Additionally, the epitopes of cytotoxic T lymphocyte (CTL) were analyzed by the NetCTL-1.2 server. The results showed that α-helices, extended strands, random coils and β-turns accounted for 84.82 %, 6.60 %, 5.56 % and 3.01 % in EgPmy’s secondary structure, respectively. A total of 29 linear B-cell epitopes and 6 conformational epitopes were identified together with 25 CTL epitopes. The CTL epitope 709KLEEAEAFA717 showed a high potential to elicit CTL response. These results suggested that EgPmy has a strong immunogenicity, which could serve as a reference for the development of EgPmy-based epitope vaccine against CE.

<italic>In silico</italic> characterization of <italic>Echinococcus granulosus</italic> paramyosin nucleotide sequence for the development of epitope vaccine against cystic echinococcosis

Jiangsu Key Laboratory of Immunity and Metabolism

Department of Pathogenic Biology and Immunology, Laboratory of Infection and Immunity

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

 characterization of  paramyosin nucleotide sequence for the development of epitope vaccine against cystic echinococcosis

The paramyosin (Pmy) protein has been presented as a potential vaccine candidate against Schistosoma spp. However, it remains elusive whether it works in...

In silico characterization of Echinococcus granulosus paramyosin nucleotide sequence for the development of epitope vaccine against cystic echinococcosis

Publicado en línea: 01 nov 2017

Páginas: 275 - 283

Recibido: 30 nov 2016

Aceptado: 29 jun 2017

DOI: https://doi.org/10.1515/helm-2017-0041

Palabras clave
paramyosin, antigen epitope, vaccine, bioinformatics

© 2017 Y.-J. Lu et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

In silico characterization of Echinococcus granulosus paramyosin nucleotide sequence for the development of epitope vaccine against cystic echinococcosis

Publicado en línea: 01 nov 2017

Páginas: 275 - 283

Recibido: 30 nov 2016

Aceptado: 29 jun 2017

DOI: https://doi.org/10.1515/helm-2017-0041

Palabras claveparamyosin, antigen epitope, vaccine, bioinformatics

© 2017 Y.-J. Lu et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
paramyosin, antigen epitope, vaccine, bioinformatics