Identification of dermatophytes by arbitrarily primed PCR
Article Category: Original article
Online veröffentlicht: 31. Jan. 2017
Seitenbereich: 291 - 298
DOI: https://doi.org/10.5372/1905-7415.0903.397
Schlüsselwörter
© 2015 Majid Zarrin, Zahra Salehi, Ali Zarei Mahmoudabadi
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Background
Dermatophytosis is a superficial infection caused by filamentous fungi belonging to the following three genera: Microsporum, Trichophyton and Epidermophyton. Arbitrarily primed PCR (AP-PCR) is a rapid and sensitive procedure for the diagnosis of the fungal species.
Objectives
To identify various dermatophyte species as rapidly and precisely as possible.
Methods
Fifty-two clinical dermatophyte isolates from ten species were recovered from samples obtained the Department of Medical Mycology and patients in different parts of Iran. All 52 dermatophyte isolates tested belonged to any of Trichophyton, Microsporum, or Epidermophyton genera. Four random primers, OPAA11, OPU15, OPAA17, and OPD18, were used in this study.
Results
The results indicated that all 10 dermatophyte species displayed distinct DNA band patterns after amplification with the random primers OPAA11 and OPU15. Nine species of dermatophytes were distinguished with the random primer OPAA17 using a different DNA band pattern. AP-PCR amplified different PCR products using primer OPD18.
Conclusions
AP-PCR offers a convenient solution to the problems encountered in the recognition of morphological features of dermatophyte species in comparison with conventional morphological methods. This approach represents technological progress in identification of dermatophytosis.