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Identification of dermatophytes by arbitrarily primed PCR

Majid Zarrin
Majid Zarrin
, 
Zahra Salehi
Zahra Salehi
 und 
Ali Zarei Mahmoudabadi
Ali Zarei Mahmoudabadi
   | 31. Jan. 2017
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Article Category: Original article
Online veröffentlicht: 31. Jan. 2017
Seitenbereich: 291 - 298
DOI: https://doi.org/10.5372/1905-7415.0903.397
Schlüsselwörter
© 2015 Majid Zarrin, Zahra Salehi, Ali Zarei Mahmoudabadi
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

PCR products of dermatophyte species with random primer OPAA11Lanes: M1, 100 bp ladder; 1, Epidermophyton floccosum; 2, Microsporum canis; 3, M. gypseum; 4, M. ferrugineum; 5, Trichophyton mentagrophytes; 6, T. rubrum; 7, T. verrucosum; 8, T. tonsurans; 9, T. violaceum; 10, T . schoenleinii; Neg, no template DNA; M2, 1 kb ladder
PCR products of dermatophyte species with random primer OPAA11Lanes: M1, 100 bp ladder; 1, Epidermophyton floccosum; 2, Microsporum canis; 3, M. gypseum; 4, M. ferrugineum; 5, Trichophyton mentagrophytes; 6, T. rubrum; 7, T. verrucosum; 8, T. tonsurans; 9, T. violaceum; 10, T . schoenleinii; Neg, no template DNA; M2, 1 kb ladder

Figure 2

PCR products of dermatophyte species with random primer OPU15. Lanes: M1, 100 bp ladder; 1, Epidermophyton floccosum; 2, Microsporum canis; 3, M. gypseum; 4, M. ferrugineum; 5, Trichophyton mentagrophytes; 6, T. rubrum; 7, T. verrucosum; 8, T. tonsurans; 9, T. violaceum; 10, T. schoenleinii; Neg, no template DNA; M2, 1 kb ladder
PCR products of dermatophyte species with random primer OPU15. Lanes: M1, 100 bp ladder; 1, Epidermophyton floccosum; 2, Microsporum canis; 3, M. gypseum; 4, M. ferrugineum; 5, Trichophyton mentagrophytes; 6, T. rubrum; 7, T. verrucosum; 8, T. tonsurans; 9, T. violaceum; 10, T. schoenleinii; Neg, no template DNA; M2, 1 kb ladder

Figure 3

PCR products of dermatophyte species with random primer OPAA17. Lanes: M1, 100 bp ladder; 1, Epidermophyton floccosum; 2, Microsporum canis; 3, M. gypseum; 4, M. ferrugineum; 5, Trichophyton mentagrophytes; 6, T. rubrum; 7, T. verrucosum; 8, T. tonsurans; 9, T. violaceum; 10, T. schoenleinii; Neg, no template DNA; M2, 1 kb Ladder
PCR products of dermatophyte species with random primer OPAA17. Lanes: M1, 100 bp ladder; 1, Epidermophyton floccosum; 2, Microsporum canis; 3, M. gypseum; 4, M. ferrugineum; 5, Trichophyton mentagrophytes; 6, T. rubrum; 7, T. verrucosum; 8, T. tonsurans; 9, T. violaceum; 10, T. schoenleinii; Neg, no template DNA; M2, 1 kb Ladder

Figure 4

PCR products of dermatophyte species with random primer OPD18. Lanes: M1, 100 bp ladder; 1, Epidermophyton floccosum; 2, Microsporum canis; 3, M. gypseum; 4, M. ferrugineum; 5, Trichophyton mentagrophytes; 6, T. rubrum; 7, T. verrucosum; 8, T. tonsurans; 9, T. violaceum, 10, T. schoenleinii; Neg, no template DNA; M2, 1 kb ladder
PCR products of dermatophyte species with random primer OPD18. Lanes: M1, 100 bp ladder; 1, Epidermophyton floccosum; 2, Microsporum canis; 3, M. gypseum; 4, M. ferrugineum; 5, Trichophyton mentagrophytes; 6, T. rubrum; 7, T. verrucosum; 8, T. tonsurans; 9, T. violaceum, 10, T. schoenleinii; Neg, no template DNA; M2, 1 kb ladder

PCR product patterns (bp) from dermatophyte species

Dermatophyte speciesNumber testedOPAA11OPU15OPAA17OPD18
Epidermophyton floccosum8350-450-650-750250-400-600-650-750-1000300-500-750-850250-350-400-600-750-900-1100-1500
Microsporum canis4350-450-650-750250-350-450-600200-300-450-700-900-1400250-350-400-450-700-750-800-1000-1500
M. gypseum8300-450-600-700-800-1000-1200-1800300-400-550-650-700-800-1000-1200-1500300-450-500-600-800-900350-450-600-700-800-900-1100-1600
M. ferrugineum2350-450-700250-350-400-600-700-900-1300-1800300-500-700300-400-500-700-800-1000-1400
Trichophyton mentagrophytes10350-750250-400-450-600-1000300-450-500-700-900350-400-750-1000
T. rubrum5350-450-650250-400-550-750-800-1000300-500-700-800-1200-1400350-400-650-800-900-1300-1500
T. verrucosum9300-400-600250-350- 800300-500-850350-450-600-700-800-900-1100-1600
T. tonsurans3350-450-600-750-850250-300-400-500-600-800-900-1400300-400-500-700-900-1200300-600-800-1000-1400-1500
T. violaceum2350-450-650250-350-400-500-600-750-1000-1200300-500-700-900-1000250-300-500-700-800-1000-1500
T. schoenleinii1350-450-550-650250-350-400-500-550-650-800-900-1000****************250-350-400-450-500-600-700-800-1000-1300-1500
Total52
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