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Knocking Down LncRNA-GAS5 Restores the Inhibition of Cuprotosis Protein FDX-1 by Acinetobacter baumannii

Zhiyuan An

Zhiyuan An

Medical Research Center, Beijing Chaoyang Hospital, Capital Medical UniversityBeijing, China
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An, Zhiyuan
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Wenyi Ding

Wenyi Ding

Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical SciencesBeijing, China
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Ding, Wenyi
  
13. Dez. 2024
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Artikel-Kategorie: SHORT COMMUNICATION
Online veröffentlicht: 13. Dez. 2024
Seitenbereich: 535 - 539
Eingereicht: 21. Juni 2024
Akzeptiert: 19. Sept. 2024
DOI: https://doi.org/10.33073/pjm-2024-040
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© 2024 Zhiyuan An et al., published by Sciendo
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Fig. 1.

Acinetobacter baumannii inhibits the expression of copper death protein FDX-1 in HEK293T cells. A) Western blot was used to detect the expression of FDX-1 protein; B) using three independent experiments as statistical analysis data, there was a significant difference in FDX-1 protein expression compared to the uninfected group, *p < 0.05.
Acinetobacter baumannii inhibits the expression of copper death protein FDX-1 in HEK293T cells. A) Western blot was used to detect the expression of FDX-1 protein; B) using three independent experiments as statistical analysis data, there was a significant difference in FDX-1 protein expression compared to the uninfected group, *p < 0.05.

Fig. 2.

LncRNA-GAS5 regulates the expression of copper death protein FDX-1. A) Transfection of HEK293T cells with pcDNA3.1 or pc3.1-GAS5 for 48 hours and detection of the FDX-1 protein expression using Western blot; B) using three independent experiments as statistical analysis data. The expression of FDX-1 showed significant differences between the pcDNA3.1-GAS5 transfection group and the pcDNA3.1 transfection group, *p < 0.05; C) transfection of HEK293T cells with sh-NC or sh-GAS5 for 48 hours was used to detect the expression of FDX-1 protein using Western blot; D) using three independent experiments as statistical analysis data. The expression of FDX-1 showed significant differences between the sh-GAS5 transfection group and the sh-NC transfection group, *p < 0.05.
LncRNA-GAS5 regulates the expression of copper death protein FDX-1. A) Transfection of HEK293T cells with pcDNA3.1 or pc3.1-GAS5 for 48 hours and detection of the FDX-1 protein expression using Western blot; B) using three independent experiments as statistical analysis data. The expression of FDX-1 showed significant differences between the pcDNA3.1-GAS5 transfection group and the pcDNA3.1 transfection group, *p < 0.05; C) transfection of HEK293T cells with sh-NC or sh-GAS5 for 48 hours was used to detect the expression of FDX-1 protein using Western blot; D) using three independent experiments as statistical analysis data. The expression of FDX-1 showed significant differences between the sh-GAS5 transfection group and the sh-NC transfection group, *p < 0.05.

Fig. 3.

LncRNA-GAS5 regulates the inhibitory effect of Acinetobacter baumannii on the expression of copper death protein FDX-1. A) After transfection of HEK293T cells with pcDNA3.1 or pcDNA3.1-GAS5 for 48 hours, the cells were infected with Acinetobacter baumannii with MOI = 10 for 4 hours, and the expression of FDX-1 protein was detected by Western blot; B) using three independent experiments as statistical analysis data, there were significant differences in FDX-1 protein expression between the empty plasmid group and the group infected with Acinetobacter baumannii alone or between the pcDNA-3.1-GAS5+Ab group and the pcDNA3.1-GAS5 transfection group, *p < 0.05 or #p < 0.05, respectively; C) after transfection of HEK293T cells with sh-NC or sh-GAS5 for 48 hours, the cells were infected with Acinetobacter baumannii with MOI = 10 for 4 hours, and the expression of FDX-1 protein was detected by Western blot; D) using three independent experiments as statistical analysis data, there were significant differences in FDX-1 protein expression between the pcDNA-3.1 group and the group infected with Acinetobacter baumannii alone, or between the sh-GAS5+Ab group and the sh-GAS5 transfection group *p < 0.05 or #p < 0.05, respectively.
LncRNA-GAS5 regulates the inhibitory effect of Acinetobacter baumannii on the expression of copper death protein FDX-1. A) After transfection of HEK293T cells with pcDNA3.1 or pcDNA3.1-GAS5 for 48 hours, the cells were infected with Acinetobacter baumannii with MOI = 10 for 4 hours, and the expression of FDX-1 protein was detected by Western blot; B) using three independent experiments as statistical analysis data, there were significant differences in FDX-1 protein expression between the empty plasmid group and the group infected with Acinetobacter baumannii alone or between the pcDNA-3.1-GAS5+Ab group and the pcDNA3.1-GAS5 transfection group, *p < 0.05 or #p < 0.05, respectively; C) after transfection of HEK293T cells with sh-NC or sh-GAS5 for 48 hours, the cells were infected with Acinetobacter baumannii with MOI = 10 for 4 hours, and the expression of FDX-1 protein was detected by Western blot; D) using three independent experiments as statistical analysis data, there were significant differences in FDX-1 protein expression between the pcDNA-3.1 group and the group infected with Acinetobacter baumannii alone, or between the sh-GAS5+Ab group and the sh-GAS5 transfection group *p < 0.05 or #p < 0.05, respectively.
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