<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Phage P2 was isolated from failed fermentation broth carried out by <italic>Lactiplantibacillus plantarum</italic> IMAU10120. A previous study in our laboratory showed that this phage belonged to the <italic>Siphoviridae</italic> family. In this study, this phage’s genomic characteristics were analyzed using whole-genome sequencing. It was revealed that phage P2 was 77.9 kb in length and had 39.28% G + C content. Its genome included 96 coding sequences (CDS) and two tRNA genes involved in the function of the structure, DNA replication, packaging, and regulation. Phage P2 had higher host specificity; many tested strains were not infected. Cell wall adsorption experiments showed that the adsorption receptor component of phage P2 might be a part of the cell wall peptidoglycan. This research might enrich the knowledge about genomic information of lactobacillus phages and provide some primary data to establish phage control measures.</p></abstract>

Phage P2 was isolated from failed fermentation broth carried out by Lactiplantibacillus plantarum IMAU10120. A previous study in our laboratory showed that this phage belonged to the Siphoviridae family. In this study, this phage’s genomic characteristics were analyzed using whole-genome sequencing. It was revealed that phage P2 was 77.9 kb in length and had 39.28% G + C content. Its genome included 96 coding sequences (CDS) and two tRNA genes involved in the function of the structure, DNA replication, packaging, and regulation. Phage P2 had higher host specificity; many tested strains were not infected. Cell wall adsorption experiments showed that the adsorption receptor component of phage P2 might be a part of the cell wall peptidoglycan. This research might enrich the knowledge about genomic information of lactobacillus phages and provide some primary data to establish phage control measures.

Whole Genome Sequence Analysis of <italic>Lactiplantibacillus plantarum</italic> Bacteriophage P2

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Whole Genome Sequence Analysis of  Bacteriophage P2

Phage P2 was isolated from failed fermentation broth carried out by Lactiplantibacillus plantarum IMAU10120. A previous study in our laboratory showed that...

Whole Genome Sequence Analysis of Lactiplantibacillus plantarum Bacteriophage P2

Article Category: Original Paper

Online veröffentlicht: 24. Sept. 2022

Seitenbereich: 421 - 428

Eingereicht: 25. Mai 2022

Akzeptiert: 22. Juli 2022

DOI: https://doi.org/10.33073/pjm-2022-037

Schlüsselwörter
phage P2, genome characteristics, functional genes, host specificity, adsorption receptor

© 2022 Hanfang Zhu et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Whole Genome Sequence Analysis of Lactiplantibacillus plantarum Bacteriophage P2

Article Category: Original Paper

Online veröffentlicht: 24. Sept. 2022

Seitenbereich: 421 - 428

Eingereicht: 25. Mai 2022

Akzeptiert: 22. Juli 2022

DOI: https://doi.org/10.33073/pjm-2022-037

Schlüsselwörterphage P2, genome characteristics, functional genes, host specificity, adsorption receptor

© 2022 Hanfang Zhu et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Schlüsselwörter
phage P2, genome characteristics, functional genes, host specificity, adsorption receptor