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Effects of Different Ambient Temperatures on Caecal Microbial Composition in Broilers

YUTING YANG
YUTING YANG
, 
XING LI
XING LI
, 
ZHENHUI CAO
ZHENHUI CAO
, 
YINGING QIAO
YINGING QIAO
, 
QIUYE LIN
QIUYE LIN
, 
JIANPING LIU
JIANPING LIU
, 
ZHIYONG ZHAO
ZHIYONG ZHAO
, 
QINGCONG AN
QINGCONG AN
, 
CHUNYONG ZHANG
CHUNYONG ZHANG
, 
HONGFU ZHANG
HONGFU ZHANG
 und 
HONGBIN PAN
HONGBIN PAN
   | 19. März 2021
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Article Category: original-paper
Online veröffentlicht: 19. März 2021
Seitenbereich: 33 - 43
Eingereicht: 26. Sept. 2020
Akzeptiert: 27. Dez. 2020
DOI: https://doi.org/10.33073/pjm-2021-001
Schlüsselwörter
© 2021 Yuting Yang et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

The relative mRNA expression level of heat shock proteins and inflammation-related genes in the liver of chickens. Different uppercase and lowercase letters indicate the significance of difference at p < 0.01 and p < 0.05, respectively. The same letters indicate no significant difference.
The relative mRNA expression level of heat shock proteins and inflammation-related genes in the liver of chickens. Different uppercase and lowercase letters indicate the significance of difference at p < 0.01 and p < 0.05, respectively. The same letters indicate no significant difference.

Fig. 2.

Composition of the dominant microbiome at the phylum level. Different uppercase and lowercase letters indicate significant differences at p < 0.01 and p < 0.05, respectively. The same letters indicate no significant difference.
Composition of the dominant microbiome at the phylum level. Different uppercase and lowercase letters indicate significant differences at p < 0.01 and p < 0.05, respectively. The same letters indicate no significant difference.

Fig. 3.

Composition of the dominant microbiome at the genus level. Different uppercase and lowercase letters indicate significant differences at p < 0.01 and p < 0.05, respectively. The same letters indicate no significant difference.
Composition of the dominant microbiome at the genus level. Different uppercase and lowercase letters indicate significant differences at p < 0.01 and p < 0.05, respectively. The same letters indicate no significant difference.

Fig. 4.

The effects of ambient temperatures on the caecal microbial communities. Principal component analysis (PCA) of the abundance profiling of microbes based on OTUs.
The effects of ambient temperatures on the caecal microbial communities. Principal component analysis (PCA) of the abundance profiling of microbes based on OTUs.

Fig. 5.

LEfSe identified the most differentially abundant microbiota between HC, MC, and LC groups.
LEfSe identified the most differentially abundant microbiota between HC, MC, and LC groups.

Fig. 6.

KEGG enrichment analyses of the caecal microbial taxa from the HC, MC, and LC groups at the L3 hierarchy.
KEGG enrichment analyses of the caecal microbial taxa from the HC, MC, and LC groups at the L3 hierarchy.

List of primer sequences for qPCR.

GeneForward primer (5’→3’)Reverse primer (5’→3’)Gene accession number
ACTBCTCGGCTGTGGTGGTGAACCATCTATGAAGGCTACGCAB495648
Hsp70TGGTGGGAATGGTGGTGTTACATCTGCTCCTGTTGGATGTCAMH422508.1
Hsp90CAGCAGCAGTATCATCTTCATCCCTGTCCTCTGGCTTTAGTTTNM001109785.1
NF-kB1AGTTCAGGATGCACCAAGAGTAGTCAACGCAGGACCTAAAGAGGAF000241
NF-KB2TGACGGTGGGATAGGTCTTGTCTGCCTGGATGGGATTGACTAU00111
IL-6CCTAGAAGGAAATGAGAATGCCTATCGTTTATGGAGAAGACCGTGAGAJ309540
TNF-αGCTTACTTCCCTTCTTCTCCTCTACATCTGACCCATCCCXM015284187

Library diversity of the 16S rRNA genes from the chicken cecum1.

Group IDHCMCLC
OTUs292 ± 22.70297 ± 42.57271 ± 52.59
Chao1334.06 ± 22.72ab351.67 ± 44.98a316.75 ± 45.95b
Observed species289.16 ± 22.77294.25 ± 42.11267.66 ± 53.71
Shannon4.93 ± 0.434.62 ± 0.644.83 ± 1.01
Simpson0.91 ± 0.030.86 ± 0.050.89 ± 0.11
PD whole tree17.32 ± 1.1817.83 ± 2.1116.64 ± 2.88
Good’s Coverage0.9983 ± 0.00010.9981 ± 0.00020.9983 ± 0.0002
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Sprache:
Englisch
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