Strains |
Streptococcus iniae HNM-1 | Isolated from infected A. sinensis | This study |
Escherichia coli DH5α | Used for cloning and propagation of plasmids | Novagen |
Escherichia coli BL21(DE3) | Used for protein expression | Invitrogen |
Salmonella typhimurium | | This study |
Staphylococcus aureus | | This study |
Acinetobacter baumannii | | This study |
Pseudomonas aeruginosa | | This study |
Plasmids |
pMD19-T | Carries ampR gene; used for cloning PCR product with A at 3’ ends | Takara |
pET 22b (+) | Carries ampR gene; used for expressing S. iniae Alanine racemase | Novagen |